Molecular identification of unusual Mycetoma agents isolated from patients in Venezuela.

Abstract:

:Mycetoma is a chronic granulomatous, subcutaneous disease endemic in tropical and subtropical countries. It is currently a health problem in rural areas of Africa, Asia and South America. Nine cases of mycetoma were analysed in a retrospective study. All isolates were identified by morphological features. The level of species identification was reached by molecular tools. Definitive identification of fungi was performed using sequence analysis of the ITS of the ribosomal DNA region and the ribosomal large-subunit D1/D2. Identification of actinomycetes was accomplished by the 16S rRNA gene sequence. Six unusual clinical isolates were identified: Aspergillus ustus, Cyphellophora oxyspora, Exophiala oligosperma, Madurella pseudomycetomatis, Nocardia farcinica and Nocardia wallacei. The prevalence of mycetoma in Venezuela remains unknown. This study represents the first report in the literature of mycetoma caused by unusual pathogens identified by molecular techniques.

journal_name

Mycoses

journal_title

Mycoses

authors

Rojas OC,León-Cachón RB,Moreno-Treviño M,González GM

doi

10.1111/myc.12579

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

129-135

issue

2

eissn

0933-7407

issn

1439-0507

journal_volume

60

pub_type

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