Comparison of morphotypic and genotypic methods for strain delineation in Candida.

Abstract:

:We compared two phenotypic methods, colony morphotyping on Sabouraudtripheniltetrazolium agar (STTZ) and serotyping, with two genotypic methods, karyotyping and Random Amplified Polymorphic DNA bands obtained by PCR amplification (RAPD-PCR), for strain delineation in 33 Candida clinical isolates and two C. albicans strains from culture collections. Analysis of isolates on STTZ showed 11 different morphotypes. In two patients there was a switch in the morphotype coincidential with a change in the susceptibility of the isolates to azole antifungals. C. albicans isolates were divided into two serotypes. Sixteen and 18 different patterns were identified among the Candida isolates by karyotyping and RAPD-PCR, respectively. No relationship was found between any of the typing methods used and the source of the isolates. The combination of karyotyping and morphotyping on STTZ yielded useful epidemiological information, since it allowed the differentiation among the Candida species studied and the discrimination of clusters within C. albicans as well as to check the capacity of a strain to generate variants with different susceptibility to some antifungals.

journal_name

Mycoses

journal_title

Mycoses

authors

Del Castillo L,Bikandi J,Nieto A,Quindós G,Sentandreu R,Pontón J

doi

10.1111/j.1439-0507.1997.tb00182.x

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

445-50

issue

11-12

eissn

0933-7407

issn

1439-0507

journal_volume

40

pub_type

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