Abstract:
:Using intraperitoneal (i. p.) infection of mice with Candida albicans we determined which parameters might be useful for characterization of virulence in this model. Upon i. p. infection of mice with two reference strains striking differences in lethality were detected. These differences in virulence corresponded with invasion of the liver and pancreas by the virulent strain and with a lack of invasion by the avirulent strain. The virulent strain was able to release high amounts of the enzymes alanine aminotransferase (ALT) and α-amylase (AM) from liver and pancreas into the blood plasma. Most likely, these enzymes were released by penetration of hyphae into the cytoplasm which was shown with electron microscopy. When invasion slowed down, there was also a drop in the activities of ALT and AM measured in the blood of infected mice. As both strains disseminated to the heart, kidneys, and lungs, dissemination into these organs was no reliable parameter for virulence in this model. However, only the virulent strain was able to reach the brain and to germinate in the kidneys and brain. In contrast to invasion and enzyme activities, the fungal load in the peritoneal cavity and in the neighbouring organs appeared not to be related with virulence. This may be concluded from the fact that there were no differences in the absolute colony forming units (cfu) and the length of persistence of both strains when similar inocula were used. We conclude that the ability of a given strain of C. albicans to invade neighbouring organs, to reach the brain upon dissemination and germination in the brain and kidneys may be used for measurement of virulence in this model when virulence is defined as lethality.
journal_name
Mycosesjournal_title
Mycosesauthors
Kretschmar M,Bertsch T,Göller M,Schaller M,Hof H,Nichterlein Tdoi
10.1111/j.1439-0507.1999.tb00007.xsubject
Has Abstractpub_date
1999-12-01 00:00:00pages
19-24eissn
0933-7407issn
1439-0507journal_volume
42 Suppl 2pub_type
杂志文章相关文献
MYCOSES文献大全abstract::Pityriasis versicolor (PV) is a superficial fungal infection where Malassezia species play a definite causative role, but the clinical significance of each of these species is not fully understood. The aim of our study was to analyse the prevalence of Malassezia species in PV lesions and to examine if the range of spe...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2006.01310.x
更新日期:2007-01-01 00:00:00
abstract::The prevalence of Candida species with and without denture-related stomatitis were evaluated in 167 Jordanian patients. The study revealed that 47 (28%) of the patients with denture-related stomatitis were colonized with Candida species (clinical group). Candida albicans was responsible for most cases of denture-relat...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.2003.00871.x
更新日期:2003-06-01 00:00:00
abstract::Repeated and prolonged use of fluconazole in treating candidosis leads to drug resistance. The aim of this study was to confirm the relationship between T916C (Y257H) mutation in Candida albicans ERG11 and fluconazole resistance. We replaced one copy of ERG11 with ERG11 containing the T916C mutation in C. albicans CAI...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12027
更新日期:2013-05-01 00:00:00
abstract::Dermatomycoses are contagious superficial fungal infections, which are highly prevalent in developed and developing countries. Caused by a range of Epidermophyton, Microsporum and Trichophyton species, dermatomycoses manifest on glabrous skin as 'ringworm', an annular scaly lesion with a variable inflammatory componen...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2008.01615.x
更新日期:2008-09-01 00:00:00
abstract::In 62 strains of Candida albicans cultivated from specimens of patients with recurrent vaginal candidosis or with renal transplants, the biotypes were determined according to several characters: colony morphology, production of chlamydospores, auxanogram of C- and N-substances, zymogram, growth kinetics, adherence cap...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.1990.33.11-12.527
更新日期:1990-11-01 00:00:00
abstract::Sepsis is a leading cause of death in the intensive care unit (ICU), with Candida spp. in the forefront among the important pathogens. As recent studies have shown, survival outcome is strongly influenced by adequate antifungal therapy at an early stage that is often delayed by the time lag associated with microbiolog...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2010.01885.x
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVES:Candidaemia carries a mortality of up to 40% and may be related to increasing complexity of medical care. Here, we determined risk factors for the development of candidaemia. METHODS:We conducted a prospective, multi-centre, case-control study over 12 months. Cases were aged ≥18 years with at least one bloo...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.13211
更新日期:2020-11-13 00:00:00
abstract::We used the model of continuous flow culture (cfc) to study the growth of Candida species. This model allows special test conditions: a long generation time of 15-20 hs, controlled limitation of nitrogen sources and carbohydrates, comparison of the growth under aerobic and anaerobic conditions simultaneously. These co...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Yeast-phase lysate antigens were prepared from 10 different isolates of Blastomyces dermatitidis. Comparative studies were performed using the lysate antigens in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in sera from dogs with blastomycosis and histoplasmosis. In order to evaluate th...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1997.tb00206.x
更新日期:1997-10-01 00:00:00
abstract::Despite several chemotherapeutic and preventative advances, opportunistic fungal infections remain common unintended consequences of cancer treatment. Currently, cancer patients spend most of their time between treatments at home, where they can inadvertently come across potential hazards from environmental and food s...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12167
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:To assess the diagnostic value of serum Candida mannan antigen (MN) and anti-mannan IgG and IgM antibodies for candidiasis. METHODS:This study was a prospective cohort study. Clinical data and venous blood samples from 23 medical centres in Beijing, China were collected between 1 January 2017 and 31 December...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.13035
更新日期:2020-02-01 00:00:00
abstract::In this study, the coats of 20 cats with no dermatophytosis lesions were sampled for colonization by Microsporum canis, which was isolated in 45% of cases. All cats were treated with oral terbinafine for 3 weeks. The drug was administered orally at a daily dosage 8.25 mg kg-1. Mycologic status was assessed at the end ...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::Fusarium infections are increasingly being encountered in immunocompromised patients. Fusarium solani accounts for nearly half of these infections. A specific nested PCR (nPCR) assay has been developed by using DNA isolated from several Fusarium species and other common fungi. Furthermore, DNA samples isolated from br...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2008.01657.x
更新日期:2010-01-01 00:00:00
abstract::Poor clinical outcome and complicated neurological complications illustrate the severity of bone and joint infections with Aspergillus species. Host predisposing conditions are immunosuppression, intravenous drug use, a variety of chronic underlying diseases and prior surgical interventions. Nosocomial infections may ...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12165
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:Cryptococcal meningitis accounts for 15% of all AIDS mortality globally. Most cases in low- and middle-income countries are treated with fluconazole monotherapy, which is associated with a high mortality. New available therapies are needed. Short-course amphotericin B has been shown to be a safe and efficien...
journal_title:Mycoses
pub_type: 临床试验,杂志文章
doi:10.1111/myc.12995
更新日期:2019-12-01 00:00:00
abstract::The in vitro hair perforation test as described by Ajello and George, was applied to eight species of Chrysosporium and one species each of Microsporum and Trichophyton. Six of them were found to perforate hair while four were negative. Chr. keratinophilum, M. gypseum and Tr. vanbreuseghemii were able to perforate and...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1989.tb02255.x
更新日期:1989-07-01 00:00:00
abstract::The infection of the capillitium of an 8-year old child due to Microsporum canis is described. The child occasionally visited its mother working at an animal farm. The focus of the infection was found in farm rabbits infected by M. canis. A contamination of the environment (cages and floors) by the above mentioned der...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::New antifungal agents are needed to match the currently increasing rate of systemic fungal infections and the development of resistant fungal strains. This appears possible by the introduction of second generation azole antifungals which potently inhibit ergosterol synthesis, but also by a partial synthetic echinocand...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::We report a case of cerebral mucormycosis in a 28-year-old male who was affected by chronic myeloid leukaemia and underwent allogeneic bone marrow transplantation. Nine months post-transplantation, he was admitted to the hospital with fever, bilateral eyelid oedema and neutropenia. X-ray analysis showed numerous areas...
journal_title:Mycoses
pub_type: 信件
doi:10.1111/j.1439-0507.2009.01689.x
更新日期:2010-05-01 00:00:00
abstract::It has always been difficult to treat onychomycosis due to decrease ability of topical agents to penetrate the nail and reach the affected nail bed. Oral antifungal have shown good response but due to longer duration course it has potential to cause systemic side effects, leading to patient non-adherence and adverse e...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12225
更新日期:2014-12-01 00:00:00
abstract::A biofilm composed of various microorganisms including Candida is found on denture surfaces and is likely to be involved in the etiology of denture-induced stomatitis. The purpose of this study was to examine the role of hydrophobic interactions in candidal adherence to acrylic surfaces, particularly that of the hypha...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2009.01694.x
更新日期:2010-05-01 00:00:00
abstract::A case of onychomycosis with nail dystrophy due to Microsporum gypseum in a healthy 35-year-old woman is described. The infection had a 2-year history, occurring in the site of a nail trauma sustained in a fall from a horse. Diagnosis was based on mycological examination (direct microscope observation and culture). Cl...
journal_title:Mycoses
pub_type: 信件
doi:10.1111/j.1439-0507.2006.01248.x
更新日期:2006-07-01 00:00:00
abstract::Interdigital ulcer is an exceptionally rare condition while erosio interdigitalis blastomycetica is common for candidiasis. Four Chinese patients with Candida interdigital ulcers were reported. The exudates were examined directly and cultured for fungi. Skin biopsies were stained with haematoxylin-eosin and periodic a...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2011.02023.x
更新日期:2011-11-01 00:00:00
abstract::This study was carried out to evaluate the utility of serological tests in the diagnosis of candidal infections in non-neutropenic critically ill patients. A prospective study was carried out in a 10-bed general intensive care unit; all patients with at least one organic sample with Candida spp. were included. A thera...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.2001.00616.x
更新日期:2001-01-01 00:00:00
abstract::A case of tinea capitis due to Microsporum gypseum in an adult is described. An otherwise healthy 69-year-old woman presented two large patches of slightly erythematous scaling alopecia localized on the vertex and on the left parietal region of the scalp. The only subjective sign was itching. A mycological culture was...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1998.tb00331.x
更新日期:1998-05-01 00:00:00
abstract::The adherence and dissociation of Candida albicans, C. tropicalis, Streptococcus mutans and S. sanguis to six substrates including hydroxylapatite (HAP) which exhibit various hydrophobicity, was examined by the use of a bioluminescent adenosine triphosphate (ATP) assay. Dissolution of HAP by C. albicans or S. mutans w...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.0933-7407.2003.00888.x
更新日期:2003-12-01 00:00:00
abstract::Invasive aspergillosis has increasingly been recognised to cause significant morbidity and mortality in immunocompromised patients. Fever unresponsive to broad-spectrum antibiotics is the earliest and most common sign of an invasive fungal infection. As invasive Aspergillus infections are usually acquired by inhalatio...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1997.tb00559.x
更新日期:1997-01-01 00:00:00
abstract::Two soil isolates of Microsporum gypseum were studied for the production of extracellular proteases. Both the strains secreted protease on glucose-gelatin medium. The enzyme activity peaked on day 15 at 28 °C. Asparagine repressed protease yield. Sugars caused catabolite repression of protease formation. Protease acti...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2010.01871.x
更新日期:2011-07-01 00:00:00
abstract::Summary The authors report a case of bullous tinea pedis caused by Trichophyton violaceum with dermatophytid reaction in a 26-year-old woman who had been in Africa. Diagnosis was based on mycological examination (direct microscope observation and culture). Recovery was achieved after systemic antimycotic therapy with ...
journal_title:Mycoses
pub_type: 信件
doi:10.1111/j.1439-0507.2006.01234.x
更新日期:2006-05-01 00:00:00
abstract::Infections caused by yeast of the genus Candida are the most common fungal infections, being Candida albicans the most common isolated species among them. The rapid identification of this yeast is mostly based on the production of germ tube in human or animal serum. This study describes the use of 12 different liquid ...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2007.01359.x
更新日期:2007-07-01 00:00:00