Ultrastructural changes in Paracoccidioides brasiliensis yeast cells attenuated by gamma irradiation.

Abstract:

:Paracoccidioides brasiliensis is a thermally dimorphic fungus agent of paracoccidioidomycosis, a deep-seated systemic infection of humans with high prevalence in Latin America. Until now no vaccine has been reported. Ionizing radiation can be used to attenuate pathogens for vaccine development and we have successfully attenuated yeast cells of P. brasiliensis by gamma irradiation. The aim of the present study was to examine at ultrastructural level the effects of gamma irradiation attenuation on the morphology of P. brasiliensis yeast cells. Paracoccidioides brasiliensis (strain Pb-18) cultures were irradiated with a dose of 6.5 kGy. The irradiated cells were examined by scanning and also transmission electron microscopy. When examined 2 h after the irradiation by scanning electron microscopy, the 6.5 kGy irradiated cells presented deep folds or were collapsed. These lesions were reversible since when examined 48 h after irradiation the yeast had recovered the usual morphology. The transmission electron microscopy showed that the irradiated cells plasma membrane and cell wall were intact and preserved. Remarkable changes were found in the nucleus that was frequently in a very electrondense form. An extensive DNA fragmentation was produced by the gamma irradiation treatment.

journal_name

Mycoses

journal_title

Mycoses

authors

Demicheli MC,Goes AM,de Andrade AS

doi

10.1111/j.1439-0507.2007.01389.x

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

397-402

issue

5

eissn

0933-7407

issn

1439-0507

pii

MYC1389

journal_volume

50

pub_type

杂志文章

相关文献

MYCOSES文献大全
  • Primary prophylaxis of invasive fungal infections with posaconazole or itraconazole in patients with acute myeloid leukaemia or high-risk myelodysplastic syndromes undergoing intensive cytotoxic chemotherapy: A real-world comparison.

    abstract::This is an observational-retrospective study comparing the real-world outcomes associated with posaconazole vs itraconazole as prophylaxis treatments. Two hundred and ninety-three patient admissions attributable to 174 patients were included in the study. Patients were treated with itraconazole (n = 114 admissions; 39...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12728

    authors: Tormo M,Pérez-Martínez A,Calabuig M,Hernández-Boluda JC,Amat P,Navarro D,Solano C

    更新日期:2018-03-01 00:00:00

  • A 20-year survey of tinea faciei.

    abstract::Tinea faciei is an uncommon dermatophytosis affecting the glabrous skin of the face. Between 1988 and 2007 at the Dermatology Department of Cagliari University, 107 cases of tinea faciei have been diagnosed, involving 72 females and 35 males, aged 2-72 years. Incidence peaks were observed between 6 and 15 years (48.59...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2009.01748.x

    authors: Nicola A,Laura A,Natalia A,Monica P

    更新日期:2010-11-01 00:00:00

  • Rhinocerebral zygomycosis in a young girl undergoing allogeneic stem cell transplantation for severe aplastic anaemia.

    abstract::We report on a 10-year-old girl with severe aplastic anaemia who developed rhinocerebral infection caused by Absidia corymbifera and a possible co-infection caused by Alternaria alternata. Despite prolonged neutropenia, therapy with liposomal amphotericin B and posaconazole improved the clinical condition. Subsequentl...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01300.x

    authors: Sörensen J,Becker M,Porto L,Lambrecht E,Schuster T,Beske F,Rickerts V,Klingebiel T,Lehrnbecher T

    更新日期:2006-01-01 00:00:00

  • Treatment of vaginal candidosis with fluconazole.

    abstract::In patients with vulvovaginal mycosis systemic treatment is being discussed for eradication of possible fungal reservoirs, therefore, the efficacy and toleration of fluconazole, a triazole-derivative, has been investigated in the course of an open, comparative study. 104 patients with clinical, microscopic and cultura...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:

    authors: Herzog RE,Ansmann EB

    更新日期:1989-04-01 00:00:00

  • African histoplasmosis: osteomyelitis of the radius.

    abstract::Bone lesions in African histoplasmosis caused by Histoplasma capsulatum var, duboisii, without other signs of the infection, are often misdiagnosed as cancer. A case of isolated osteomyelitis due to this fungus in a 30-year-old woman is described. Diagnostic and therapeutic problems of isolated bone lesions in African...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00309.x

    authors: Onwuasoigwe O,Gugnani HC

    更新日期:1998-03-01 00:00:00

  • The changing trends and profile of pneumocystosis mortality in the United States, 1999-2014.

    abstract::Pneumocystosis (PCP) mortality in the U.S. has received less attention in recent years. This study describes recent trends in mortality and the estimated burden of PCP in the U.S., using the national multiple cause of death data during 1999-2014. PCP mortality rates were calculated for age, sex, race and year. Demogra...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12636

    authors: Wickramasekaran RN,Jewell MP,Sorvillo F,Kuo T

    更新日期:2017-09-01 00:00:00

  • Cryptococcosis in cirrhotic patients.

    abstract::The objective of the present study was to report on three distinct forms of presentation of Cryptococcus neoformans infection in three cirrhotic patients. One patient had disseminated cryptococcosis with detection of the fungus in ascitic fluid, cerebrospinal fluid and blood; the second patient had pleural involvement...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2004.01064.x

    authors: França AV,Carneiro M,dal Sasso K,Souza Cda S,Martinelli A

    更新日期:2005-01-01 00:00:00

  • Case report. Monascus purpureus--a new fungus of allergologic relevance.

    abstract::Anaphylactic reactions to food containing allergens in the consumption or preparation of food are well known. However, allergy in the preparation of sausages has rarely been described. In the present study a 26-year-old-butcher was investigated who had a severe anaphylactic reaction developing sneezing, rhinitis, conj...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:

    authors: Hipler UC,Wigger-Alberti W,Bauer A,Elsner P

    更新日期:2002-02-01 00:00:00

  • Preliminary studies of the antifungal activities of some medicinal plants against Basidiobolus and some other pathogenic fungi.

    abstract::The antifungal activities of extracts of 10 medicinal plants collected from south-eastern parts of Nigeria were tested against seven pathogenic fungi using the broth dilution and agar plate methods. All the extracts at 1:10 dilution inhibited the growth of Basidiobolus haptosporus and B. ranarum but did not inhibit th...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1995.tb00048.x

    authors: Nwosu MO,Okafor JI

    更新日期:1995-05-01 00:00:00

  • Non-value of Aspergillus antigen detection in bronchoalveolar lavage fluids of patients undergoing bone marrow transplantation.

    abstract::A commercially available antigen assay (Pastorex Aspergillus) was used to detect Aspergillus antigen in serial bronchoalveolar lavage (BAL) fluids and sera of patients undergoing bone marrow transplantation (six patients with autopsyproven aspergillosis and 10 control patients without evidence of fungal infection). As...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1996.tb00155.x

    authors: Rath PM,Oeffelke R,Müller KD,Ansorg R

    更新日期:1996-09-01 00:00:00

  • Evaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species.

    abstract::CHROMagar Candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei. We evaluated this medium and compared it with a reference medium, Sabouraud glucose agar, for the presump...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.1999.00406.x

    authors: Willinger B,Manafi M

    更新日期:1999-04-01 00:00:00

  • A rescue therapy with a combination of caspofungin and liposomal amphotericin B or voriconazole in children with haematological malignancy and refractory invasive fungal infections.

    abstract::Combination treatment of paediatric invasive fungal infections (IFIs) has rarely been reported. A total of 17 children with 19 IFI episodes were enrolled in the study. The median age of the patients was 5.3 (range 0.5-17) years. IFI was classified as proven in 4, probable in 12 and possible in 3 episodes. These patien...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2009.01808.x

    authors: Yilmaz D,Balkan C,Ay Y,Akin M,Karapinar B,Kavakli K

    更新日期:2011-05-01 00:00:00

  • Direct fluconazole susceptibility testing of positive Candida blood cultures by flow cytometry.

    abstract::The standard methods for yeast susceptibility testing require 24-48 h of incubation. As there has been an increase in incidence of non-albicans Candida species, the clinician is very often wary of initiating therapy with fluconazole until a final susceptibility report is generated, especially when treating very sick p...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2007.01466.x

    authors: Rudensky B,Broide E,Berko N,Wiener-Well Y,Yinnon AM,Raveh D

    更新日期:2008-05-01 00:00:00

  • Correlation between phospholipase of Candida albicans and resistance to fluconazole.

    abstract::The objective of this study was to compare phospholipase production between fluconazole-resistant and fluconazole-susceptible strains of Candida albicans in order to explore the relationship between resistance to antifungal drugs and virulence of C. albicans. Fifteen each of fluconazole-resistant (MIC ≥ 64 μg ml(-1)) ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2011.02024.x

    authors: Ying S,Chunyang L

    更新日期:2012-01-01 00:00:00

  • Cryptococcosis: the 1981-2000 epidemic.

    abstract::The annual incidence of cryptococcosis during 1981-2000 was determined in subscribers of a large integrated health care program in Northern California and in those among them who were HIV positive. The incidence of cryptococcosis had been measured in this setting in the previous decade. The 20-year incidence per milli...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2004.01082.x

    authors: Friedman GD,Jeffrey Fessel W,Udaltsova NV,Hurley LB

    更新日期:2005-03-01 00:00:00

  • First isolation of Trichophyton raubitschekii (syn. T. rubrum) in Europe.

    abstract::Trichophyton raubitschekii is a rare dermatophyte which belongs to the Trichophyton rubrum species complex. Since 1981, only a few cases of dermatophytosis due to this anthropophilic causative agent were published. In this paper the authors report the first cases of Tinea corporis caused by Trichophyton raubitschekii ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:

    authors: Tietz HJ,Hopp M,Gräser Y

    更新日期:2002-02-01 00:00:00

  • Invasive metastasing pulmonary aspergillosis under polychemotherapy.

    abstract::We report on a female patient who received a polychemotherapy because of an extensive pemphigus vulgaris. Under this therapy, the general condition of the patient worsened. Noncharacteristic symptoms, such as dyspnoea and tachycardia occurred. In spite of comprehensive, also invasive diagnostics, pulmonary foci demons...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1994.tb00393.x

    authors: Sima D,Tietz HJ,Reisshauer H,Buchholtz I,Schulze P,Meyer R

    更新日期:1994-11-01 00:00:00

  • Detection of circulating galactomannan by Pastorex Aspergillus in experimental invasive aspergillosis.

    abstract::The performance of Pastorex Aspergillus, a new latex agglutination test for the detection of circulating galactomannan in the serum of patients with invasive aspergillosis, was evaluated in a blind trial in standardized guinea-pig models of invasive aspergillosis and other invasive mycoses. In these animal models, the...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.1990.33.2.61

    authors: Van Cutsem J,Meulemans L,Van Gerven F,Stynen D

    更新日期:1990-02-01 00:00:00

  • Clinical isolates of Trichophyton rubrum are completely inhibited by photochemical treatment with a γ-cyclodextrin formulation of curcuminoids.

    abstract:INTRODUCTION:It was shown previously that dermatophytes can markedly be inhibited by a photochemical treatment with curcumin. This kind of photo-inactivation needs to be improved, however, because curcumin is poorly water-soluble. Therefore, a new water-soluble γ-cyclodextrin formulation of curcuminoids was tested for ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13051

    authors: Brasch J,Beck-Jendroschek V,Walther G,Rubbel D

    更新日期:2020-04-01 00:00:00

  • Detection of IgM and IgG antibodies to Cryptococcus neoformans proteins in blood donors and HIV patients with active cryptococcosis.

    abstract::The serological responses to Cryptococcus neoformans proteins of blood donors and HIV patients with active cryptococcosis from a tropical region were investigated in this study. Exposure to C. neoformans, an organism ubiquitous in the environment, contributes to the antibody responses observed in the blood donors. IgG...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2008.01549.x

    authors: Chai HC,Tay ST

    更新日期:2009-03-01 00:00:00

  • Pityriacitrin--a potent UV filter produced by Malassezia furfur and its effect on human skin microflora.

    abstract::In Malassezia furfur, tryptophan as the main nitrogen source induces production of the potent ultraviolet-absorbing indole compound pityriacitrin. An in vitro study about the effects of pityriacitrin on other human skin microorganisms is presented, with special focus on Candida albicans and staphylococci in which its ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01265.x

    authors: Machowinski A,Krämer HJ,Hort W,Mayser P

    更新日期:2006-09-01 00:00:00

  • HIV-associated disseminated histoplasmosis in western French Guiana, 2002-2012.

    abstract::Disseminated histoplasmosis (DH) is the most current revelation mode of AIDS in French Guiana. We describe the clinical and paraclinical presentation of DH, diagnostic tools, evolution and factors associated with 1-year mortality in HIV-infected patients from western French Guiana. Microbiologically proven AIDS-relate...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12293

    authors: Putot A,Perrin S,Jolivet A,Vantilcke V

    更新日期:2015-03-01 00:00:00

  • Deep-seated fungal diseases in the South Pacific, especially in New Caledonia.

    abstract::The main deep-seated fungal diseases and their encountered pathology in New Caledonia and other islands of the South Pacific are reviewed (1970-96). Cryptococcosis is encountered in all islands of the South Pacific, Australia and Papua New Guinea, with a predominance of variety gattii, which is associated with some sp...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.1998.tb00372.x

    authors: Monchy D,Huerre MR,de Bièvre C

    更新日期:1998-01-01 00:00:00

  • An atypical isolate of Paracoccidioides brasiliensis.

    abstract::An atypical strain of Paracoccidioides brasiliensis was obtained from a patient with disseminated paracoccidioidomycosis. This isolate, which has been maintained in the laboratory for 4 years by successive subcultures (30-40 days) in Fava Netto medium, produces cerebriform colonies characteristic of the yeast phase of...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1995.tb00024.x

    authors: Hamdan JS,Ferrari TC

    更新日期:1995-11-01 00:00:00

  • Two new media Pinus halepensis seed agar and blackberry agar for rapid identification of Cryptococcus neoformans.

    abstract::Cryptococcus neoformans is an encapsulated yeast-like fungus that causes life-threatening infections, particularly in immunocompromised patients. The formation of brown pigment on many media described in the literature, such as that in Niger seed (Guizotia abyssinica) agar, has been used to identify C. neoformans. The...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2010.01877.x

    authors: Mseddi F,Sellami A,Sellami H,Cheikhrouhou F,Makni F,Ayadi A

    更新日期:2011-07-01 00:00:00

  • Central nervous system infection due to Cryptococcus gattii sensu lato in India: Analysis of clinical features, molecular profile and antifungal susceptibility.

    abstract::Cryptococcus gattii species complex has evolved as a pathogen in the last two decades causing infection among both immunocompetent and immunocompromised hosts. We aimed to analyse the clinical features of CNS infection caused by C. gattii sensu lato, molecular and antifungal susceptibility profile of this pathogen. Ca...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12656

    authors: Lahiri Mukhopadhyay S,Bahubali VH,Manjunath N,Swaminathan A,Maji S,Palaniappan M,Parthasarathy S,Chandrashekar N

    更新日期:2017-11-01 00:00:00

  • Anti-Malassezia furfur antibodies in the population.

    abstract::The authors carried out research into anti-Malassezia precipitating antibodies in the population, specifying the distribution by age and sex. A total of 868 serum specimens from subjects of both sexes, aged between 0 and 80 years, were studied. An immunoelectroprecipitation reaction was used using a M. furfur culture ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00337.x

    authors: Faggi E,Pini G,Campisi E,Gargani G

    更新日期:1998-09-01 00:00:00

  • [Fungal nucleic acid detection for invasive aspergillosis].

    abstract::A universal PCR-assay for the detection of fungal DNA was compared with microscopy and culture for the diagnosis of invasive aspergillosis using 78 samples from 42 patients. Eighteen patients were suffering from invasive aspergillosis, 5 patients were colonized with Aspergillus in the respiratory tract, 19 patients di...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00605.x

    authors: Rimek D,Garg AP,Kappe R,Sonntag HG

    更新日期:1998-01-01 00:00:00

  • The continuous flow culture as an in vitro model in experimental mycology.

    abstract::We used the model of continuous flow culture (cfc) to study the growth of Candida species. This model allows special test conditions: a long generation time of 15-20 hs, controlled limitation of nitrogen sources and carbohydrates, comparison of the growth under aerobic and anaerobic conditions simultaneously. These co...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:

    authors: Bernhardt H,Zimmermann K,Knoke M

    更新日期:1999-01-01 00:00:00

  • Extracellular protease expression in Microsporum gypseum complex, its regulation and keratinolytic potential.

    abstract::Two soil isolates of Microsporum gypseum were studied for the production of extracellular proteases. Both the strains secreted protease on glucose-gelatin medium. The enzyme activity peaked on day 15 at 28 °C. Asparagine repressed protease yield. Sugars caused catabolite repression of protease formation. Protease acti...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2010.01871.x

    authors: Singh CJ

    更新日期:2011-07-01 00:00:00