An evaluation of seven methods of testing in vitro susceptibility of clinical yeast isolates to fluconazole.

Abstract:

:Four commercially available in vitro test systems (Candifast, E-test, Mycototal and spiral-gradient end point method), agar diffusion with 25-micrograms fluconazole paper test discs and 15-micrograms test tablets and agar dilution were compared with the microbroth dilution method for fluconazole susceptibility testing of 145 clinical isolates. In addition, the culture media provided or recommended by the manufacturers of the test systems were compared with high-resolution (HR) antifungal test medium. With all currently available culture media, growth problems (inhibition or delayed growth of the clinical isolates) occurred with solid or semisolid media. With minor improvements, HR medium demonstrated the most reproducible and comparable results (supplementation with asparagine and deletion of sodium hydrogen carbonate). The best correlation with microdilution was obtained by the agar dilution method (> 95% concordance) followed by the spiral-gradient end point method (85%), Candifast (83%), Mycototal (81%) and the E-test (78%). Regression analysis demonstrated good correlation between agar diffusion and micro-/agar dilution (r > 0.9).

journal_name

Mycoses

journal_title

Mycoses

authors

Schmalreck AF,Kottmann I,Reiser A,Ruffer U,Scharr E,Vanca E

doi

10.1111/j.1439-0507.1995.tb00065.x

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

359-68

issue

9-10

eissn

0933-7407

issn

1439-0507

journal_volume

38

pub_type

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