Virulence attributes and genetic variability of oral Candida albicans and Candida tropicalis isolates.

Abstract:

:The wide spectrum of candidiasis and its clinical importance encourage the research with the purpose of clarifying the mechanisms of pathogenicity and identification of virulence factors of Candida sp. Therefore, the aim of this study was to verify the adhesion capacity, protease activity and genotypic diversity of oral C. albicans and C. tropicalis isolates. The adhesion ability to the extracellular matrix glycoproteins laminin and fibronectin was evaluated using the ELISA technique. The research of proteases was carried out in agar plate containing bovine albumin and through a quantitative method in buffer solution containing haemoglobin. Intra and interspecies polymorphisms was verified through random amplified polymorphic DNA (RAPD) technique. All C. albicans and C. tropicalis isolates binded to immobilised laminin and fibronectin. Ca33 and Ct13 isolates had relative adhesion index significantly higher than the other isolates for both glycoproteins (P < 0.001). Protease activity was observed in all isolates of C. albicans using either the semi-quantitative or quantitative assay. The protease activity of C. tropicalis was better detected through the quantitative assay. The genotypic diversity by RAPD revealed a heterogeneous population in both species. Nevertheless, C. tropicalis presented higher genetic variability than C. albicans strains.

journal_name

Mycoses

journal_title

Mycoses

authors

da Costa KR,Ferreira JC,Lavrador MA,Baruffi MD,Candido RC

doi

10.1111/j.1439-0507.2011.02125.x

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

e97-e105

issue

3

eissn

0933-7407

issn

1439-0507

journal_volume

55

pub_type

杂志文章

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