Abstract:
BACKGROUND:Bone allografts carry a risk of infection, so terminal sterilization by gamma irradiation at 25kGy is recommended; but is deleterious to bone quality. Contemporary bone banking significantly reduces initial allograft bioburden, questioning the need to sterilize at 25kGy. METHODS:We inoculated allograft bone with Staphylococcus epidermidis and Bacillus pumilus, then exposed them to gamma irradiation at 0, 5, 10, 15, 20 and 25kGy. Mechanical and biological properties of allografts were also assessed. Our aim was to determine an optimal dose that achieves sterility assurance while minimizing deleterious effects on allograft tissue. RESULTS:20-25kGy eliminated both organisms at concentrations from 10(1) to 10(3)CFU, while 10-15kGy sterilized bone samples to a bioburden concentration of 10(2)CFU. Irradiation did not generate pro-inflammatory bone surfaces, as evidenced by macrophage activation, nor did it affect attachment or proliferation of osteoblasts. At doses ≥10kGy, the toughness of cortical bone was reduced (P<0.05), and attachment and fusion of osteoclasts onto irradiated bone declined at 20 and 25kGy (P<0.05). There was no change in collagen cross-links, but a significant dose-response increase in denatured collagen (P<0.05). CONCLUSIONS:Our mechanical and cell biological data converge on 15kGy as a threshold for radiation sterilization of bone allografts. Between 5 and 15kGy, bone banks can undertake validation that provides allografts with an acceptable sterility assurance level, improving their strength and biocompatibility significantly. CLINICAL RELEVANCE:The application of radiation sterilization doses between 5 and 15kGy will improve bone allograft mechanical performance and promote integration, while retaining sterility assurance levels. Improved quality of allograft bone will promote superior clinical outcomes.
journal_name
Bonejournal_title
Boneauthors
Nguyen H,Cassady AI,Bennett MB,Gineyts E,Wu A,Morgan DA,Forwood MRdoi
10.1016/j.bone.2013.07.036subject
Has Abstractpub_date
2013-11-01 00:00:00pages
194-200issue
1eissn
8756-3282issn
1873-2763pii
S8756-3282(13)00298-6journal_volume
57pub_type
杂志文章相关文献
BONE文献大全abstract::It is well known that physical inactivity leads to loss of muscle mass, but it also causes bone loss. Mechanistically, osteoclastogenesis and bone resorption have recently been shown to be regulated by vibration. However, the underlying mechanism behind the inhibition of osteoclast formation is yet unknown. Therefore,...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.08.020
更新日期:2013-12-01 00:00:00
abstract::Sclerosteosis and van Buchem disease are two rare bone sclerosing dysplasias caused by genetic defects in the synthesis of sclerostin. In this article we review the demographic, clinical, biochemical, radiological, and histological characteristics of patients with sclerosteosis and van Buchem disease that led to a bet...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2016.10.010
更新日期:2017-03-01 00:00:00
abstract::Recent studies have indicated a critical role for vascular endothelial growth factor (VEGF) during the process of endochondral ossification, in particular in coupling cartilage resorption with bone formation. Therefore, we studied the chemoattractive and proliferative properties of human VEGF-A on primary human osteob...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00690-1
更新日期:2002-03-01 00:00:00
abstract::Avian tibial dyschondroplasia is a disorder of endochondral ossification which results in the accumulation of noncalcified, avascular cartilage. We have investigated the changes in cell proliferation and enzyme activities within specific cell types in the growth plate of chickens with differing severity of the disease...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(92)90362-z
更新日期:1992-01-01 00:00:00
abstract::In 1994, the World Health Organization published diagnostic criteria for osteoporosis. Since then, many new technologies have been developed for the measurement of bone mineral at multiple skeletal sites. The information provided by each assessment will describe the clinical characteristics, fracture risk and epidemio...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2007.11.001
更新日期:2008-03-01 00:00:00
abstract::SOST encodes sclerostin, an inhibitor of bone formation that antagonizes canonical Wnt signaling. Variations of SOST expression have an impact on bone mineral density (BMD) and bone strength. We hypothesized that genetic and epigenetic DNA modifications have an impact on SOST gene expression. By analyzing 43 bone samp...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.08.004
更新日期:2016-11-01 00:00:00
abstract::Inhaled corticosteroids are the cornerstone of the modern therapy for asthma. In recent years, inhaled corticosteroids have been used in higher doses than previously. This has caused concern about possible osteoporotic side-effects. We studied bone mineral densities (BMDs) in 19 non-smoking women (mean age 53 years, r...
journal_title:Bone
pub_type: 临床试验,杂志文章
doi:10.1016/8756-3282(94)90309-3
更新日期:1994-11-01 00:00:00
abstract::Matrix vesicles (MVs) are membrane organelles found in the extracellular matrix of calcifying cells, which contain matrix processing enzymes and regulate the extracellular environment via action of these enzymes. It is unknown whether MVs are also exosomic mediators of cell-cell communication via transfer of RNA mater...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.03.018
更新日期:2016-07-01 00:00:00
abstract::Short-term changes in biochemical markers of bone turnover (bone markers) have been suggested as predictors of long-term response in bone mass during antiresorptive treatment. In the Danish cohort (n = 306) of the Early Postmenopausal Intervention Cohort (EPIC) Study (n = 1609) of oral alendronate (ALN) for prevention...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00168-6
更新日期:2003-07-01 00:00:00
abstract::Five strains of inbred mice were found to have variations in bone mass although they were similar in body weight. Two of these strains, C57BL/6J and A/J, were studied in greater detail and the former had more bone in both femur and tibia. The increased bone mass was associated with larger quadriceps muscles in the C57...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(00)00164-2
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND:Vertebral fracture (VF) is the most common osteoporotic fracture in postmenopausal women, although most VFs are subclinical. Prevalent VFs are a significant predictor of subsequent fracture and therefore, identification of VF improves the identification of those with high fracture risk. The aim of present st...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115358
更新日期:2020-07-01 00:00:00
abstract::The effects of glucocorticoid (GC) excess, thyrotoxicosis, and hypothyroidism on linear growth indicate that growth plate chondrocytes are exquisitely sensitive to GC and thyroid hormone (T(3)). Murine ATDC5 cells undergo chondrogenesis in vitro and were used to evaluate the effects of dexamethasone (Dex) and T(3) on ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00855-4
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Since July 1, 2006 bone mineral density (BMD) testing has been reimbursed for osteoporosis screening under specific clinical conditions in France. Concurrently, drug coverage has been extended to all anti-osteoporotic treatments in women with low BMD. It was thus expected that the utilisation of BMD testing ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.06.026
更新日期:2010-10-01 00:00:00
abstract::Intracortical remodeling in the adult skeleton removes and replaces areas of compact bone that have sustained microdamage. Although studies have been performed in animal species in which there is an existing baseline of remodeling activity, laboratory rodents have been considered to have limited suitability as models ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00104-5
更新日期:1998-09-01 00:00:00
abstract::Paget's disease of bone (PDB) is a chronic bone metabolic disorder. Currently, PDB is the second most frequent bone disorder. PDB is a focal disorder affecting the skeleton segmentally but the cause of which is unknown. It has been hypothesised that somatic mutations could be responsible for the mosaicism described in...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.04.003
更新日期:2018-07-01 00:00:00
abstract::The potency of magnetic resonance imaging (MRI) to measure the exact extent of osteonecrosis of the femoral head (ONFH) remains uncertain. The objective of this study was to determine if the volume of necrosis assessed with MRI accurately reflects the volume of architectural mineral alterations in osteonecrosis of the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115099
更新日期:2020-01-01 00:00:00
abstract::Paramagnetic manganese can be employed as a calcium surrogate to sensitize the magnetic resonance imaging (MRI) technique to the processing of calcium during the bone formation process. At low doses, after just 48h of exposure, osteoblasts take up sufficient quantities of manganese to cause marked reductions in the wa...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.02.014
更新日期:2011-05-01 00:00:00
abstract::Epigenetic regulatory proteins support mammalian development, cancer, aging and tissue repair by controlling many cellular processes including stem cell self-renewal, lineage-commitment and senescence in both skeletal and non-skeletal tissues. We review here our knowledge of epigenetic regulatory protein complexes tha...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115659
更新日期:2021-02-01 00:00:00
abstract::The topological changes in vertebral cancellous bone were estimated in vertebrae from calcium-restricted ovariectomized Sinclair S-1 minipigs, a recently described animal model of cancellous osteopenia. Connectivity was estimated using unbiased stereological principles in disector pairs of sections from the first lumb...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(95)00116-u
更新日期:1995-06-01 00:00:00
abstract::Growing evidence suggests that positive associations between fat mass (FM) and bone mineral density (BMD) are mediated by not only biomechanical but also biochemical factors. Adiponectin is a novel adipocyte-derived hormone that regulates energy homeostasis and has anti-inflammatory and anti-atherogenic effects. Unlik...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00237-0
更新日期:2003-10-01 00:00:00
abstract::In elderly subjects and in particular in those with osteoporosis the evidence on age related volume changes of the hip is still very limited. Even less is known about bone changes of the femoral head. The aim of this study is to explore associations of bone size of the femoral head and neck with age in postmenopausal ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115545
更新日期:2020-10-01 00:00:00
abstract::Matrix Extracellular Phospho-glycoprotEin (MEPE) and proteases are elevated and PHEX is defective in HYP. PHEX prevents proteolysis of MEPE and release of a protease-resistant MEPE-ASARM peptide, an inhibitor of mineralization (minhibin). Thus, in HYP, mutated PHEX may contribute to increased ASARM peptide release. Mo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.09.015
更新日期:2005-01-01 00:00:00
abstract::The purpose of our study was to investigate the recovery of bone disease in celiac patients during 5 years of a gluten-free diet. The study group consisted of 28 newly diagnosed celiac patients (9 men, 19 women) recruited between 1990 and 1991. Six patients withdrew from the 5-year follow-up. Compliance with the glute...
journal_title:Bone
pub_type: 临床试验,杂志文章
doi:10.1016/s8756-3282(99)00171-4
更新日期:1999-09-01 00:00:00
abstract::Sclerostin monoclonal antibody (Scl-Ab) has been shown to increase bone mass and bone strength by stimulating bone formation in an ovariectomy-induced bone loss rat model. The purpose of this study was to determine the effects of Scl-Ab in a rat immobilization/disuse model in which there was both a decrease in bone fo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.09.009
更新日期:2011-02-01 00:00:00
abstract::The skeleton is characterized by anatomic heterogeneity of metabolic turnover. Site-dependent differences in hormonal effects seem likely. Hyporesponsiveness of osteoclasts to parathyroid hormone (PTH) and probably calcitriol was recently demonstrated in the fifth lumbar vertebra of a rat model with moderate renal fai...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00502-6
更新日期:2001-09-01 00:00:00
abstract::Mitochondria play a key role in cell physiology including cell differentiation and proliferation. We investigated the changes of mitochondrial biogenesis during Wnt-induced osteoblastic differentiation of murine mesenchymal C3H10T1/2 cells. Scanning electron microscopy demonstrated that activation of Wnt signaling by ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.04.593
更新日期:2010-07-01 00:00:00
abstract::Localized proton spectra of the human lumbar vertebral body were recorded in vivo at 1.5 T, using the STEAM (stimulated echo acquisition mode) pulse sequence. Thirty-seven patients (18 men, 19 women) were examined, ranging in age from 21 to 68 years (mean = 40, SD = 13). The fat fraction of the bone marrow was calcula...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(93)90239-7
更新日期:1993-03-01 00:00:00
abstract::Osteoporosis is a common complex disorder with reduced bone mineral density (BMD) and increased susceptibility to fracture. Peak BMD is one of the primary determinants of osteoporotic fracture risk, and is under substantial genetic control. Extracellular matrix, a major component of the bone, influences BMD by regulat...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.04.013
更新日期:2014-07-01 00:00:00
abstract::Six women, aged 24-67 years, had osteopenia and insufficiency fractures, which suggested a diagnosis of osteomalacia. The insufficiency fractures occurred at traditional sites for Looser zones, were multiple in number, were symmetrically distributed in three patients, and did not heal promptly. Bone mass was low, as a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(87)90073-1
更新日期:1987-01-01 00:00:00
abstract::Ultrashort echo time magnetic resonance imaging (UTE-MRI) techniques have been increasingly used to assess cortical bone microstructure. High resolution micro computed tomography (μCT) is routinely employed for validating the MRI-based assessments. However, water protons in cortical bone may reside in micropores small...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.03.013
更新日期:2019-06-01 00:00:00