Strong stimulation triggers full fusion exocytosis and very slow endocytosis of the small dense core granules in carotid glomus cells.

Abstract:

:Chemosensory glomus cells of the carotid bodies release transmitters, including ATP and dopamine mainly via the exocytosis of small dense core granules (SDCGs, vesicular diameter of ∼100 nm). Using carbon-fiber amperometry, we showed previously that with a modest uniform elevation in cytosolic Ca2+ concentration ([Ca2+]i of ∼0.5 µM), SDCGs of rat glomus cells predominantly underwent a "kiss-and-run" mode of exocytosis. Here, we examined whether a larger [Ca2+]i rise influenced the mode of exocytosis. Activation of voltage-gated Ca2+ channels by a train of voltage-clamped depolarizations which elevated [Ca2+]i to ∼1.6 μM increased the cell membrane capacitance by ∼2.5%. At 30 s after such a stimulus, only 5% of the added membrane was retrieved. Flash photolysis of caged-Ca2+ (which elevated [Ca2+]i to ∼16 μM) increased cell membrane capacitance by ∼13%, and only ∼30% of the added membrane was retrieved at 30 s after the UV flash. When exocytosis and endocytosis were monitored using the two-photon excitation and extracellular polar tracer (TEP) imaging of FM1-43 fluorescence in conjunction with photolysis of caged Ca2+, almost uniform exocytosis was detected over the cell's entire surface and it was followed by slow endocytosis. Immunocytochemistry showed that the cytoplasmic densities of dynamin I, II and clathrin (key proteins that mediate endocytosis) in glomus cells were less than half of those in adrenal chromaffin cells, suggesting that a lower expression of endocytotic machinery may underlie the slow endocytosis in glomus cells. An analysis of the relative change in the signals from two fluorescent dyes that simultaneously monitored the addition of vesicular volume and plasma membrane surface area, suggested that with an intense stimulus, SDCGs of glomus cells underwent full fusion without any significant "compound" exocytosis. Therefore, during a severe hypoxic challenge, glomus granules undergo full fusion for a more complete release of transmitters.

journal_name

J Neurogenet

journal_title

Journal of neurogenetics

authors

Tse A,Lee AK,Takahashi N,Gong A,Kasai H,Tse FW

doi

10.1080/01677063.2018.1497629

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

267-278

issue

3

eissn

0167-7063

issn

1563-5260

journal_volume

32

pub_type

杂志文章
  • The serotonin transporter expression in Drosophila melanogaster.

    abstract::The serotonin transporter is an important regulator of serotonergic signaling. In order to analyze where the Drosophila melanogaster ortholog of the mammalian serotonin transporter (dSERT) is expressed in the nervous system, a dSERT antibody serum was used. Ectopic expression studies and loss of function analysis reve...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2011.553002

    authors: Giang T,Ritze Y,Rauchfuss S,Ogueta M,Scholz H

    更新日期:2011-03-01 00:00:00

  • The pattern of early neuronal differentiation in Drosophila melanogaster.

    abstract::Based on the staining of Drosophila embryos with neuron-specific monoclonal antibodies we describe the differentiation of the earliest neurons in the central nervous system. The metameric array undergoes a number of changes during development that distinguish several morphological units: metameres, neuromeres and gang...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677068609106856

    authors: Canal I,Ferrús A

    更新日期:1986-09-01 00:00:00

  • Distinct mechanisms of action of the Lozenge locus in Drosophila eye and antennal development are suggested by the analysis of dominant enhancers.

    abstract::The development of the olfactory sense organs on the antenna of the fruit fly Drosophila utilises mechanisms distinct from those used in the rest of the adult peripheral nervous system. Lozenge (lz) is the only locus hither-to identified as required for the development of antennal sense organs. In addition to effects ...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677069509083460

    authors: Gupta BP,Rodrigues V

    更新日期:1995-12-01 00:00:00

  • Adaptive adjustment of the generalization-discrimination balance in larval Drosophila.

    abstract::Learnt predictive behavior faces a dilemma: predictive stimuli will never 'replay' exactly as during the learning event, requiring generalization. In turn, minute differences can become meaningful, prompting discrimination. To provide a study case for an adaptive adjustment of this generalization-discrimination balanc...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2010.498066

    authors: Mishra D,Louis M,Gerber B

    更新日期:2010-09-01 00:00:00

  • A novel mutation in the gene encoding noggin is not causative in human neural tube defects.

    abstract::Neural tube defects (NTD) are a common birth defect, with both genetic and environmental contributions to their etiology. In mouse, null mutations in Noggin result in fully-penetrant NTDs. We investigated Noggin for mutations that may predispose to human NTDs in 202 NTD cases. One variant allele was identified in a ma...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:

    authors: Bauer KA,George TM,Enterline DS,Stottmann RW,Melvin EC,Siegel D,Samal S,Hauser MA,Klingensmith J,Nye JS,Speer MC,Neural Tube Defects Collaborative Group.

    更新日期:2002-01-01 00:00:00

  • Dopamine DRD2 polymorphism (DRD2/ANNK1-Taq1A) is not a significant risk factor in writer's cramp.

    abstract::Writers' cramp is a movement disorder with dystonic co-contraction of fingers and hand during writing and is part of the clinical spectrum of focal dystonias. Previous studies showed reduced striatal dopamine receptor D2 (DRD2) availability in dystonia. The expression of D2 receptors is modulated by a DRD2/ANKK1-Taq1A...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677063.2016.1238916

    authors: Zeuner KE,Acewicz A,Knutzen A,Dressler D,Lohmann K,Witt K

    更新日期:2016-01-01 00:00:00

  • Behavioral phenotypic properties of a natural occurring rat model of congenital stationary night blindness with Cacna1f mutation.

    abstract::Cacna1f gene mutation could lead to incomplete congenital stationary night blindness (iCSNB) disease. The CSNB-like phenotype rat is a spontaneous rat model caused by Cacna1f gene mutation. The present study explored the phenotypic properties of behavior performance in CSNB rats further. The vision-related behaviors o...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2012.684416

    authors: An J,Wang L,Guo Q,Li L,Xia F,Zhang Z

    更新日期:2012-09-01 00:00:00

  • Optomotor-blind in the development of the Drosophila HS and VS lobula plate tangential cells.

    abstract::The horizontal system and vertical system cells of the dipteran optic lobes are well understood regarding their physiology and role in visually guided behavior. Little is known, however, about their development. Drosophila optomotor-blind (omb) is required for the development of the HS/VS cells which are lacking in th...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2014.917645

    authors: Sen A,Grimm S,Hofmeyer K,Pflugfelder GO

    更新日期:2014-09-01 00:00:00

  • Temperature signaling underlying thermotaxis and cold tolerance in Caenorhabditis elegans.

    abstract::Caenorhabditis elegans has a simple nervous system of 302 neurons. It however senses environmental cues incredibly precisely and produces various behaviors by processing information in the neural circuit. In addition to classical genetic analysis, fluorescent proteins and calcium indicators enable in vivo monitoring o...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677063.2020.1734001

    authors: Takeishi A,Takagaki N,Kuhara A

    更新日期:2020-09-01 00:00:00

  • Tethering membrane fusion: common and different players in myoblasts and at the synapse.

    abstract::Membrane fusion is essential for the communication of membrane-defined compartments, development of multicellular organisms and tissue homeostasis. Although membrane fusion has been studied extensively, still little is known about the molecular mechanisms. Especially the intercellular fusion of cells during developmen...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章,评审

    doi:10.3109/01677063.2014.936014

    authors: Önel SF,Rust MB,Jacob R,Renkawitz-Pohl R

    更新日期:2014-09-01 00:00:00

  • From form to function: the ways to know a neuron.

    abstract::The shape of a neuron, its morphological signature, dictates the neuron's function by establishing its synaptic partnerships. Here, we review various anatomical methods used to reveal neuron shape and the contributions these have made to our current understanding of neural function in the Drosophila brain, especially ...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章,评审

    doi:10.1080/01677060802610604

    authors: Meinertzhagen IA,Takemura SY,Lu Z,Huang S,Gao S,Ting CY,Lee CH

    更新日期:2009-01-01 00:00:00

  • RNA editing in the Drosophila DMCA1A calcium-channel alpha 1 subunit transcript.

    abstract::Messenger RNA editing of transcripts encoding voltage-sensitive ion channels has not been extensively analyzed--least of all in Drosophila, for which several channel-encoding genes are known. Previous sequence studies of D. melanogaster's cacophony gene, which encodes an alpha 1 calcium-channel subunit called Dmca1A, ...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677069809108560

    authors: Smith LA,Peixoto AA,Hall JC

    更新日期:1998-11-01 00:00:00

  • The UNC-119 family of neural proteins is functionally conserved between humans, Drosophila and C. elegans.

    abstract::C. elegans animals mutant for the unc-119 gene exhibit movement, sensory and behavioral abnormalities. Consistent with a nervous system role, unc-119 reporter genes are expressed throughout the C. elegans nervous system. The UNC-119 protein has strong sequence similarity to the predicted protein from a human gene, HRG...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677060009084494

    authors: Maduro MF,Gordon M,Jacobs R,Pilgrim DB

    更新日期:2000-01-01 00:00:00

  • Isogenic autosomes to be applied in optimal screening for novel mutants with viable phenotypes in Drosophila melanogaster.

    abstract::Most insertional mutagenesis screens of Drosophila performed to date have not used target chromosomes that have been checked for their suitability for phenotypic screens for viable phenotypes. To address this, we have generated a selection of stocks carrying either isogenized second chromosomes or isogenized third chr...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677060591007155

    authors: Sharma P,Asztalos Z,Ayyub C,de Bruyne M,Dornan AJ,Gomez-Hernandez A,Keane J,Killeen J,Kramer S,Madhavan M,Roe H,Sherkhane PD,Siddiqi K,Silva E,Carlson JR,Goodwin SF,Heisenberg M,Krishnan K,Kyriacou CP,Partridge L,

    更新日期:2005-04-01 00:00:00

  • Noradrenergic gating of long-lasting synaptic potentiation in the hippocampus: from neurobiology to translational biomedicine.

    abstract::Altered synaptic strength underlies information storage in neural circuits. Neuromodulatory transmitters such as norepinephrine (NE) facilitate long-lasting synaptic plasticity by recruiting and modifying multiple molecular elements of synaptic signaling, including specific transmitter receptors, intracellular protein...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章,评审

    doi:10.1080/01677063.2018.1497630

    authors: Nguyen PV,Gelinas JN

    更新日期:2018-09-01 00:00:00

  • Kinase-mediated signaling cascades in mood disorders and antidepressant treatment.

    abstract::Kinase-mediated signaling cascades regulate a number of different molecular mechanisms involved in cellular homeostasis, and are viewed as one of the most common intracellular processes that are robustly dysregulated in the pathophysiology of mood disorders such as depression. Newly emerged, rapid acting antidepressan...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章,评审

    doi:10.1080/01677063.2016.1245303

    authors: Yuan LL,Wauson E,Duric V

    更新日期:2016-01-01 00:00:00

  • Dopamine modulates female sexual receptivity in Drosophila melanogaster.

    abstract::Newly eclosed Drosophila melanogaster flies were systemically depleted of dopamine by feeding an inhibitor of the biosynthetic enzyme, tyrosine hydroxylase, and analyzed for abnormalities in courtship behavior. Dopamine-depleted females were significantly less receptive to males than were control females, although mal...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677069809167259

    authors: Neckameyer WS

    更新日期:1998-03-01 00:00:00

  • Cell lineage relationships in the development of the mammalian CNS. II. Bilateral independence of CNS clones.

    abstract::This report contains additional observations on the cell lineage relationships of the motor neurons of the facial nucleus and of the cerebellar Purkinje cells of the mouse. These cell populations were quantitatively analyzed in the mosaic brains of experimental aggregation chimeras. The cell markers used to perform th...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677068409107092

    authors: Herrup K,Wetts R,Diglio TJ

    更新日期:1984-12-01 00:00:00

  • Conditioning deficits of CaM-kinase transgenic Drosophila melanogaster in a new excitatory courtship assay.

    abstract::Courtship suppression is an associative conditioning procedure in Drosophila melanogaster that is ethologically based and capable of being tested on individual flies. We have expanded the range of the courtship conditioning by developing an excitatory procedure in which male flies learn to associate a novel odor with ...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:

    authors: Broughton SJ,Tully T,Greenspan RJ

    更新日期:2003-01-01 00:00:00

  • Karl-Friedrich Fischbach's first publications and their impact in the biological sciences.

    abstract::Predictions from the theory of transfection, Karl-Friedrich Fischbach's first paper, were confirmed 20 years later. Also a model, proposed already in 1977 by Karl-Friedrich and colleagues, to explain the nonmonotonous dependence on light intensity of phototaxis in Drosophila, finds support in recent studies of functio...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2013.873430

    authors: Spatz HC

    更新日期:2014-09-01 00:00:00

  • The evolution and development of neural superposition.

    abstract::Visual systems have a rich history as model systems for the discovery and understanding of basic principles underlying neuronal connectivity. The compound eyes of insects consist of up to thousands of small unit eyes that are connected by photoreceptor axons to set up a visual map in the brain. The photoreceptor axon ...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章,评审

    doi:10.3109/01677063.2014.922557

    authors: Agi E,Langen M,Altschuler SJ,Wu LF,Zimmermann T,Hiesinger PR

    更新日期:2014-09-01 00:00:00

  • Drosophila rosA gene, which when mutant causes aberrant photoreceptor oscillation, encodes a novel neurotransmitter transporter homologue.

    abstract::The Drosophila receptor oscillation A (rosA) mutations, which cause electroretinogram (ERG) defects, including oscillations, were localized to the 24F4-25A2 region of chromosome 2L. Genomic fragments from this region, isolated from bacteriophage P1 clones, included those that detect transcriptional defects in rosA mut...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677069609107063

    authors: Burg MG,Geng C,Guan Y,Koliantz G,Pak WL

    更新日期:1996-12-01 00:00:00

  • A touching story.

    abstract::A slide taped to a window at the Woods Hole Marine Biology Laboratory was my first introduction to the touch receptor neurons of the nematode Caenorhabditis elegans. Studying these cells as a postdoc with Sydney Brenner gave me a chance to work with John Sulston on a fascinating set of neurons. I would never have gues...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677063.2020.1833879

    authors: Chalfie M

    更新日期:2020-09-01 00:00:00

  • The role of human-specific gene duplications during brain development and evolution.

    abstract::One of the most fascinating questions in evolutionary biology is how traits unique to humans, such as their high cognitive abilities, erect bipedalism, and hairless skin, are encoded in the genome. Recent advances in genomics have begun to reveal differences between the genomes of the great apes. It has become evident...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2013.789512

    authors: Sassa T

    更新日期:2013-09-01 00:00:00

  • Autosomal recessive ataxia caused by three distinct gene defects in a single consanguineous family.

    abstract::Autosomal recessive cerebellar ataxias are a group of clinically and genetically heterogeneous neurodegenerative disorders. Growing data have shown that there is difficulty with genetic counseling in a deeply consanguineous population because of the presence of genetic heterogeneity in patients sharing similar phenoty...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677060802025233

    authors: Bouhlal Y,Zouari M,Kefi M,Ben Hamida C,Hentati F,Amouri R

    更新日期:2008-01-01 00:00:00

  • Individual differences in sensory responses influence decision making by Drosophila melanogaster larvae on exposure to contradictory cues.

    abstract::Animals make decisions on behavioral choice by evaluating internal and external signals. Individuals often make decisions in different ways, but the underlying neural mechanisms are not well understood. Here, we describe a system for observing the behavior of individual Drosophila melanogaster larvae simultaneously pr...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677063.2016.1202949

    authors: Koseki N,Mori S,Suzuki S,Tonooka Y,Kosugi S,Miyakawa H,Morimoto T

    更新日期:2016-01-01 00:00:00

  • Linkage analysis in a family with dominantly inherited torsion dystonia: exclusion of the pro-opiomelanocortin and glutamic acid decarboxylase genes and other chromosomal regions using DNA polymorphisms.

    abstract::A search for the defective gene causing torsion dystonia has been carried out in a family manifesting an autosomal dominant mode of inheritance of this movement disorder. Complete neurologic examination and establishment of lymphoblast lines have been carried out for over 50 members. Linkage analysis, using cloned DNA...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677068609106846

    authors: Breakefield XO,Bressman SB,Kramer PL,Ozelius L,Moskowitz C,Tanzi R,Brin MF,Hobbs W,Kaufman D,Tobin A

    更新日期:1986-05-01 00:00:00

  • Inter-tissue networks between the basal forebrain, hippocampus, and prefrontal cortex in a model for depression caused by disturbed sleep.

    abstract::Disturbances in sleep are encountered in the majority of patients with depressive disorder. To elucidate the molecular mechanisms behind this relationship, we examined gene expression changes in a rodent model for disturbed sleep and depression. The animals were treated with daily injections of clomipramine to affect ...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2012.694932

    authors: Lagus M,Gass N,Saharinen J,Savelyev S,Porkka-Heiskanen T,Paunio T

    更新日期:2012-09-01 00:00:00

  • The irre cell recognition module (IRM) protein Kirre is required to form the reciprocal synaptic network of L4 neurons in the Drosophila lamina.

    abstract::Each neuropil module, or cartridge, in the fly's lamina has a fixed complement of cells. Of five types of monopolar cell interneurons, only L4 has collaterals that invade neighboring cartridges. In the proximal lamina, these collaterals form reciprocal synapses with both the L2 of their own cartridge and the L4 collat...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.3109/01677063.2014.883390

    authors: Lüthy K,Ahrens B,Rawal S,Lu Z,Tarnogorska D,Meinertzhagen IA,Fischbach KF

    更新日期:2014-09-01 00:00:00

  • High-fat diet withdrawal modifies alcohol preference and transcription of dopaminergic and GABAergic receptors.

    abstract::The bidirectional and positive relation between the ingestion of fat and alcohol has become the subject of extensive discussion. However, this relation is more studied in animal models of binge eating with intermittent access of high-fat diet or in a model of short period of this diet consumption. Here, we developed a...

    journal_title:Journal of neurogenetics

    pub_type: 杂志文章

    doi:10.1080/01677063.2018.1526934

    authors: Martins de Carvalho L,Lauar Gonçalves J,Sondertoft Braga Pedersen A,Damasceno S,Elias Moreira Júnior R,Uceli Maioli T,Faria AMC,Brunialti Godard AL

    更新日期:2019-03-01 00:00:00