Abstract：
:During mechanical unloading, endothelial cells reduce osteogenesis and increase bone resorption. Here we describe the feedback response of endothelial cells to unloaded osteoblasts. Primary endothelial cells, ex vivo mouse aortic rings and chicken egg yolk membranes were incubated with conditioned medium from mouse primary osteoblasts (OB-CM) subjected to unit gravity or simulated microgravity, to assess its effect on angiogenesis. In vivo injection of botulin toxin A (Botox) in the quadriceps and calf muscles of C57BL/6J mice was performed to mimic disuse osteoporosis. Unloaded osteoblasts showed strong upregulation of the pro-angiogenic factor, VEGF, and their conditioned medium increased in vitro endothelial cell viability, Cyclin D1 expression, migration and tube formation, ex vivo endothelial cell sprouting from aortic rings, and in ovo angiogenesis. Treatment with the VEGF blocker, avastin, prevented unloaded OB-CM-mediated in vitro and ex vivo enhancement of angiogenesis. Bone mechanical unloading by Botox treatment, known to reduce bone mass, prompted the overexpression of VEGF in osteoblasts. The cross talk between osteoblasts and endothelial cells plays a pathophysiologic role in the response of the endothelium to unloading during disuse osteoporosis. In this context, VEGF represents a prominent osteoblast factor stimulating angiogenesis.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Veeriah V,Paone R,Chatterjee S,Teti A,Capulli Mdoi
10.1007/s00223-018-0496-zsubject
Has Abstractpub_date
2019-03-01 00:00:00pages
344-354issue
3eissn
0171-967Xissn
1432-0827pii
10.1007/s00223-018-0496-zjournal_volume
104pub_type
杂志文章abstract：:Energy-dispersive X-ray microanalysis was used to determine calcium/phosphorous (Ca/P) ratios in undecalcified teeth, and the sulfur (S) content of dentin of decalcified teeth from normal patients and patients with familial hypophosphatemia, in an attempt to determine the effect of phosphorus deficiency. The results s...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555886
更新日期：1990-12-01 00:00:00
abstract：:Experimental analysis with paired femurs provides the opportunity to study within-person differences in fracture type and associated structural side differences. We hypothesized that different fracture types in the hips of a subject are associated with structural asymmetry. Bone mineral density (BMD) and structural me...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9388-6
更新日期：2010-09-01 00:00:00
abstract：:The purpose of this study was to determine whether total serum calcium, parathyroid gland structure, and/or levels of parathyroid hormone, 1,25 and 24,25 DHCC, are altered in pigs with dental and skeletal fluorosis. Eight experimental animals receiving 2 mg F-/kg b.w. per day from age 8-14 months were compared with ei...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556714
更新日期：1986-04-01 00:00:00
abstract：:Bone turnover markers (BTMs) are widely used for the management of osteoporosis, and the premenopausal reference range is the target value for the treatment of postmenopausal osteoporosis with antiresorbing agents. Three serum BTMs (serum C-telopeptide of type I collagen [CTX], osteocalcin [OC], and N-terminal propept...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9126-5
更新日期：2008-05-01 00:00:00
abstract：:This study examined effects of bone bonding and nonbonding implants on parameters associated with matrix vesicle-mediated primary bone formation, matrix vesicle alkaline phosphatase and phospholipase A2 specific activities, and phosphatidylserine content. Tibia marrow ablation followed by implantation of KG-Cera, Mina...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296676
更新日期：1992-12-01 00:00:00
abstract：:Vitamin D supplements are often recommended to restore sufficiency, although the adherence to treatment is low. This study assessed vitamin D status at different time intervals following the cessation of treatment. The database of Clalit-Health-Services (CHS), a not-for-profit HMO covering more than half of the Israel...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9536-7
更新日期：2011-11-01 00:00:00
abstract：:Using data from the Peking Vertebral Fracture Study, we conducted a longitudinal cohort study to investigate the association between type 2 diabetes mellitus (T2DM) and the risk of incident fractures, especially of vertebral fractures (VFs), and we also examined the modifying effect of body mass index (BMI) on this as...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00598-x
更新日期：2019-11-01 00:00:00
abstract：:Several rare inherited disorders have been described that show phenotypic overlap with Paget's disease of bone (PDB) and in which PDB is a component of a multisystem disorder affecting muscle and the central nervous system. These conditions are the subject of this review article. Insertion mutations within exon 1 of t...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-019-00520-5
更新日期：2019-05-01 00:00:00
abstract：:Calcium deficiency is a major etiological determinant of rickets in Nigerian children and is accompanied by undermineralization of the developing bone matrix which is composed largely of type I collagen. We have assessed types I and III collagen metabolism by measuring the circulating concentrations of teh N- and C-te...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900301
更新日期：1997-08-01 00:00:00
abstract：:Dickkopf-1 (DKK1) and sclerostin are antagonists of the Wnt/β-catenin pathway and decreased expression of either results in increased bone formation and mass. As both affect the same signaling pathway, we aimed to elucidate the redundancy and/or compensation of sclerostin and DKK1. Weekly sclerostin antibody (Scl-Ab) ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0420-6
更新日期：2018-09-01 00:00:00
abstract：:Genome-wide association studies (GWAS) have demonstrated that genetic variation at the MADS box transcription enhancer factor 2, polypeptide C (MEF2C) locus is robustly associated with bone mineral density, primarily at the femoral neck. MEF2C is a transcription factor known to operate via the Wnt signaling pathway. O...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-013-9824-5
更新日期：2014-04-01 00:00:00
abstract：:In this study, we demonstrated that type I collagen matrix induced the expression of osteoblastic phenotypes of bone marrow cells, and that antibone sialoprotein (BSP) monoclonal antibody suppressed the expression of these phenotypes. On the other hand, BSP accelerated the expression of osteoblastic phenotypes of bone...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230010078
更新日期：2000-05-01 00:00:00
abstract：:Patients put at bedrest for medical reasons lose 1-2% of spinal bone mineral per week. Losses of this magnitude during even short-term space flights of a few months would pose a serious limitation and require countermeasures. The spinal bone mineral (L2-L4) was determined in 6 healthy males (precision = 2%) before and...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555226
更新日期：1987-11-01 00:00:00
abstract：:The feasibility of two noninvasive methods [dual photon absorptiometry (DPA) and dual energy X-ray absorptiometry (DXA)] for prediction in vivo of local variations of trabecular bone strength within the proximal tibia was evaluated in 14 cadaveric knees. Trabecular bone strength was measured using an osteopenetrometer...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900131
更新日期：1996-10-01 00:00:00
abstract：:We examined the role of sleep, growth hormone (GH), and parathyroid hormone [PTH(1-84)] as regulators of the diurnal rhythm of the osteoblastic bone marker, serum osteocalcin (OC). Nine normal subjects were followed with hourly blood sampling during one 24-hour period with normal sleep pattern, and one 24-hour period ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555845
更新日期：1991-12-01 00:00:00
abstract：:We have compared two components of bacterial cell walls, muramyl dipeptide (MDP) and lipopolysaccharide (LPS), for their effects on bone resorption as measured by the release of previously incorporated 45Ca. MDP is the smallest active component of peptidoglycan, whereas LPS is the active component of endotoxin. Fetal ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411269
更新日期：1982-07-01 00:00:00
abstract：:1,25(OH)2D3 was found to regulate its own receptor levels via an increase in corresponding mRNA levels in human osteoblast-like osteosarcoma cells (MG-63). In addition, exposure of the cells for 24h to dexamethasone, estradiol, retinoic acid, or triiodothyronine resulted in a dose-dependent accumulation of hVDR mRNA. ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555101
更新日期：1991-01-01 00:00:00
abstract：:There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900111
更新日期：1996-09-01 00:00:00
abstract：:A liposome-centered endogenous precipitation method was used to investigate the effect of ultrafilterable fragments from the enzymatic digestion of rat chondrosarcoma aggrecan on the formation of insoluble calcium phosphate salts in buffered solutions at pH 7.4 and 22 degrees C. Unlike the intact aggrecan and its majo...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00425872
更新日期：1994-09-01 00:00:00
abstract：:In several studies on patients with rheumatoid arthritis, an association of bone loss with a persistently high disease activity has been found. The aim of our study was to investigate the relation between disease activity and serum levels of vitamin D metabolites, parathyroid hormone (PTH), and parameters of bone turn...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900416
更新日期：1998-03-01 00:00:00
abstract：:Parameters of mineral metabolism were examined in 6 patients with moderately severe anticonvulsant drug-induced osteomalacia. Compared to 15 matched controls, the patients exhibited significantly reduced serum calcium, inorganic phosphate, and 25-hydroxyvitamin D concentration, elevated serum alkaline phosphatase and ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02441155
更新日期：1979-03-13 00:00:00
abstract：:Amelogenins are the most abundant secreted proteins in developing dental enamel. These evolutionarily-conserved proteins have important roles in enamel mineral formation, as mutations within the amelogenin gene coding region lead to defects in enamel thickness or mineral structure. Because of extensive alternative spl...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-002-0036-7
更新日期：2003-11-01 00:00:00
abstract：:Osteoblast differentiation is predominantly regulated by the WNT/β-catenin signaling (canonical WNT pathway), which, together with bone morphogenetic proteins, acts as the master regulator of osteogenesis. The recent characterization of the canonical WNT pathway in the regulation of bone modeling and remodeling provid...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-013-9749-z
更新日期：2013-08-01 00:00:00
abstract：:We examined the osteogenesis process in transforming growth factor beta 1 (TGF-beta 1)-treated neonatal and adult rats, aiming to investigate the age difference in the effect of TGF-beta 1 on mesenchymal cell differentiation. Recombinant human (rh) TGF-beta 1 (20 and 200 ng) was injected onto the outer periostea of th...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF01321890
更新日期：1993-08-01 00:00:00
abstract：:Recent evidence of the occurrence of brushite in newly formed bone mineral prompted a study of the solubility properties of brushite:apatite mixtures under physiological conditions and the influence on them of pH, lactate, pyruvate, and, particularly, noncollagenous bone proteins (NCBPs). Brushite alone was surprising...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411226
更新日期：1982-03-01 00:00:00
abstract：:Two rheumatic syndromes associated with deposition of calcium phosphate crystals in soft tissues of the shoulder have prompted us to study the cellular mechanisms of calcium phosphate crystal solubilization. Synthetic 45Ca labeled calcium phosphate crystal aggregates were solubilized by cultured human fibroblasts or m...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405384
更新日期：1984-12-01 00:00:00
abstract：:Transcriptional regulation of the postnatal skeleton is incompletely understood. Here, we determined the consequence of loss of early growth response gene 1 (EGR-1) on bone properties. Analyses were performed on both the microscopic and molecular levels utilizing micro-computed tomography (micro-CT) and Fourier transf...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9486-0
更新日期：2011-07-01 00:00:00
abstract：:Although treatment with intranasal salmon calcitonin (sCT) has been shown to effectively inhibit postmenopausal bone loss, there is still controversy over both timing and the duration of its application. In an open prospective study, we therefore assessed the effect of shortterm intranasal sCT on postmenopausal bone t...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02508638
更新日期：1996-04-01 00:00:00
abstract：:The purpose of this research was to address the following question: How is the solubility of fluoride-pretreated carbonated apatite (CAP) in aqueous acidic media related to the equilibrium solution fluoride and/or the CAP adsorbed fluoride levels? A CAP sample prepared by a precipitation method at 70 degrees C contain...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-1069-z
更新日期：2003-03-01 00:00:00
abstract：:There is increasing evidence that 1,25 dihydroxyvitamin D3 acts directly to differentiate and activate cells of mononuclear phagocyte lineage (MNP). However, it seems possible that in bone marrow this hormone may modulate the differentiation of MNP via the factor elaborated by the stromal cells or the macrophages. We ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556592
更新日期：1986-01-01 00:00:00
