Abstract:
:The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake venom in the egg yolk of immunized hens. We have adapted a reported process of antibody purification from egg yolks, and achieved 90% antibody purity. The customized technique consisted of the removal of lipids from distilled water-diluted egg yolks by a freeze-thaw sequence. The specific immunoglobulins were present in the egg yolk for up to 180 days postimmunization. Therefore, by means of small venom quantities, a significant amount of immunoglobulins were found in an adequately purified state (The obtained material contained about 90% pure IgY). The antigen binding of the immunoglobulins was detected by a double immunodiffusion test. Titers of antibodies in the yolk were estimated with a serum protection assay (Median effective dose = ED50) (ED50= 477 mg/kg). Given that breeding hens is economically feasible, egg gathering is noninvasive and the purification of IgY antibodies is quick and easy, chicken immunization is an excellent alternative for the production of polyclonal antibodies. To the best of our knowledge, this is the first coral snake antivenom prepared in birds.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Aguilar I,Sánchez EE,Girón ME,Estrella A,Guerrero B,Rodriguez-Acosta FAdoi
10.1590/S0036-46652014000100009subject
Has Abstractpub_date
2014-01-01 00:00:00pages
61-6issue
1eissn
0036-4665issn
1678-9946pii
S0036-46652014000100061journal_volume
56pub_type
杂志文章abstract::Two cases of ectopic cutaneous schistosomiasis are described. Both patients presented with abdominal papular skin lesions, which on biopsy were found to contain granulomas with Schistosoma mansoni eggs. Twenty-five other cases were retrieved from the literature. Most patients were female, mean age 24.9 year, with a pr...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
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journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
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journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
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journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
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journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
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pub_type: 临床试验,杂志文章,随机对照试验
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