Abstract:
:Several methods have been used for the preservation of fungi, all of them presenting advantages and disadvantages. The choice of the methods depends upon the laboratory availabilities, time of preservation, genetic stability of the cultures and other factors. In this work the results obtained through the utilization of Castellani's method (preservation in distilled water) for the maintenance of 174 strains belonging to the "Micoteca do Instituto de Medicina Tropical de São Paulo" are presented. These strains were analyzed after 6, 12, 18 and 24 months, with regard to the percentage of viability taking into consideration the rates of growth and contamination. The smallest percentage of viability occurred in the group of the actinomycetes (50 to 100%) and the largest one in the group of the yeasts (near 100%). According to other authors, the Castellani's method, besides being simple and economically feasible for small size laboratories, yields good results.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Rodrigues EG,Lírio VS,Lacaz Cda Ssubject
Has Abstractpub_date
1992-03-01 00:00:00pages
159-65issue
2eissn
0036-4665issn
1678-9946journal_volume
34pub_type
杂志文章abstract::Escherichia coli causes various ailments such as septicemia, enteritis, foodborne illnesses, and urinary tract infections which are of concern in the public health field due to antibiotic resistance. Silver nanoparticles (AgNP) are known for their biocompatibility and antibacterial activity, and may prove to be an alt...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s1678-9946201860018
更新日期:2018-01-01 00:00:00
abstract::The development of small ultrasonographic equipment has permitted to incorporate this technique to the methods of early detection of Human Hydatidosis. 689 echographies were carried out in this experience, and 5.51% of compatible images in the whole population, and 12.24% in groups of risk (those living together with ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000400010
更新日期:1989-07-01 00:00:00
abstract::In 2018, Bhutan reported 54 cases of malaria, of which six were indigenous, 14 introduced and 34 imported. Considering the continuous reduction in the number of indigenous cases, Bhutan plans to eliminate malaria by 2025 under the Bhutan Malaria Elimination Strategy. The study was conducted to assess the presence of a...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201961052
更新日期:2019-09-12 00:00:00
abstract::Physical examination of nails was carried out in 210 elderly patients and nail scrapings were obtained from onychomycosis suggested lesions in order to determine their causative agents, incidence and clinical characteristics. Diagnostic was confirmed by the isolation of the agents from 74 patients, mainly from toe-nai...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651993000300001
更新日期:1993-05-01 00:00:00
abstract::A case of subcutaneous phaeohyphomycosis caused by Cladophialophora sp. is reported. The patient, an immunosuppressed host presented a nodule on the dorsum of the right hand which relapsed four months after excision. Dematiaceous septate hyphal and yeast like elements were seen in mycological and histological examinat...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652007000200008
更新日期:2007-03-01 00:00:00
abstract::Among the 424 serum samples examined, the prevalence of hepatitis virus infection turned out to be 89.6%, with 15.6% of HBsAg positivity. Some of the samples belonged to an afferent population and some other to workers of a West Africa rural hospital (Pop. Rep. of Benin). 27.3% of the positive subjects presented activ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000100006
更新日期:1990-01-01 00:00:00
abstract::The 4-year study (1987-1990) covered the major clinical-epidemiological characteristics of pneumonia in children as diagnosed at the emergency service of the Children's Hospital, as well as etiologies, and factors involved in the most severe cases. Etiology was determined in 47.7% of the 541 pneumonia cases, involving...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000300010
更新日期:1994-05-01 00:00:00
abstract::The authors report two cases of adrenal insufficiency secondary to infiltration of the adrenal glands by Paracoccidioides brasiliensis. The first patient had been treated for a chronic multifocal form of paracoccidiodomycosis 11 years ago. The diagnosis of the mycosis was done simultaneous with that of the adrenal ins...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652009000100008
更新日期:2009-01-01 00:00:00
abstract::[This corrects the article doi: 10.1590/s1678-9946201961041]. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 已发布勘误
doi:10.1590/S1678-9946201961041err
更新日期:2019-09-12 00:00:00
abstract::This study evaluated the whole blood immunochromatographic card test (ICT card test) in a survey performed in Northeastern Brazil. 625 people were examined by the thick blood film (TBF) and ICT card test. Residents of a non-endemic area were also tested by the whole blood card test and Og4C3. The sensitivity of the IC...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652003000300002
更新日期:2003-05-01 00:00:00
abstract::A serological screening was performed in 615 individuals aged 0-87 years, living in the city of Cordoba, Argentina to study the relationship between antibody prevalence for the SLE virus and age. A 13.98% prevalence of neutralizing antibodies was obtained and its relation to age was significantly high (p = 0.045). The...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652002000200001
更新日期:2002-03-01 00:00:00
abstract::Three autochthonous cases of cutaneous-ganglionar rickettsiosis transmitted by dogs ticks (Amblyomma maculatum in one of them) are reported. Two of the three cases were undoubtely produced by Rickettsia conorii according to the results of the specific indirect immunofluorescence technique IEF-IgM anti R. conorii. A th...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000500001
更新日期:1990-09-01 00:00:00
abstract::Neurocysticercosis (NC), the presence of Taenia solium metacestodes in tissues, is the most frequent and severe parasitic infection of the central nervous system. We investigated the presence of total IgE by an automated chemiluminescence assay in 53 paired cerebrospinal fluid (CSF) and serum samples from patients wit...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652000000200002
更新日期:2000-03-01 00:00:00
abstract::Surfacen® is an exogenous natural lung surfactant, composed by phospholipids and hydrophobic proteins, which is applied successfully in Newborn Respiratory Distress Syndrome. In this paper, in vitro activity of Surfacen® against Leishmania amazonensis is described. The product showed activity against the amastigote fo...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000400012
更新日期:2011-07-01 00:00:00
abstract::A pair matched case/control study was conducted from January 1991 to 30 June 1992 in order to define clinical and laboratory findings associated with DMAC infection in AIDS patients. Since DMAC infection is usually associated with advanced immunodeficiency, and therefore also with other opportunistic illnesses, in add...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651999000500002
更新日期:1999-09-01 00:00:00
abstract::Therapy of coccidioidomycosis continues to evolve. For primary pulmonary disease, antifungal therapy is frequently not required while prolonged courses of antifungals are generally needed for those in whom extrathoracic disseminated has occurred. Intravenous amphotericin B should be reserved for those with severe dise...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/S0036-46652015000700010
更新日期:2015-09-01 00:00:00
abstract::Twenty seven cases of neurocysticercosis, with clinical picture of acute meningitis, are described. Twenty (74.1%) patients are male; the age was 4 to 42 years (23.6 +/- 11.7 years). The etiologic diagnosis was defined by the complement fixation test (Weinberg) and/or enzyme-linked immunosorbent assay (ELISA) for cyst...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::The circulation of arboviruses throughout the world and the maintenance of lymphatic filariasis endemicity in tropical countries, combined with the lack of vaccines and specific treatments, highlight the importance of reducing the populations of mosquitoes involved in the transmission of these pathogens, Aedes aegypti...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946202062094
更新日期:2020-11-27 00:00:00
abstract::The Authors report a case of paracoccidioidomycosis in a Surui Indian patient from the state of Rondonia, Brazilian Amazon. The subject is an adult male, having been diagnosed on the basis of mycologic, serologic, and radiographic exams. The prescribed therapy was sulphamethoxazole 800 mg associated with trimethoprim ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::It is essential that healthcare providers are familiar with the full spectrum of clinical presentations of syphilis. A rare manifestation of secondary syphilis is the corymbiform (or corymbose) arrangement, in which a central greater papule is surrounded by smaller satellite lesions. Very few reports of corymbiform sy...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type:
doi:10.1590/S1678-9946201860040
更新日期:2018-08-20 00:00:00
abstract::Thirty eight paralysis cases classified as Guillain-Barré syndrome (GBS) in Brazil were analysed. In all these cases Sabin-related poliovirus vaccine strains were isolated. In most of the cases the last vaccine dose was given months or years before the onset of GBS, suggesting a persistent infection or the transmissio...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651996000100010
更新日期:1996-01-01 00:00:00
abstract::The specificity and sensitivity of the enzyme immunoassay (EIA), presently used in South America areas where hydatidosis caused by Echinococcus granulosus is endemic, was compared to two alternative EIA. One of these uses an hydatid antigen of different preparation and the other vesicular fluid of Taenia crassiceps ci...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651993000200007
更新日期:1993-03-01 00:00:00
abstract::We report the first case of human ocular sparganosis in the state of Santa Catarina, southern Brazil. A young female patient presented with three periocular moveable inflammatory masses in her right eye, during two years. By surgical excisional biopsy, a helminth larval stage was removed and identified as sparganum. C...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000100009
更新日期:2011-01-01 00:00:00
abstract::Hand-foot-and-mouth disease (HFMD) is becoming one of the extremely common airborne and contact transmission diseases in Guangzhou, southern China, leading public health authorities to be concerned about its increased incidence. In this study, it was used an ecological study plus the negative binomial regression to id...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652014000600014
更新日期:2014-11-01 00:00:00
abstract::The mutagenic activities of 16 anti-parasite drugs were screened by the Simultest in both qualitative (spot test) and quantitative (plate incorporation) assays with a Salmonella typhimurium pool composed by the indicator strains TA97, TA98, TA100 and TA102. Four anti Chagas' disease drugs (nifurtimox, benznidazole, CL...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000400006
更新日期:1990-07-01 00:00:00
abstract::Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652001000500007
更新日期:2001-09-01 00:00:00
abstract::The currently known distribution range of Achatina fulica Bowdich, 1822, in the state of São Paulo, Brazil, is presented. The record of A. fulica naturally infested with Aelurostrongylus abstrusus larvae (Railliet, 1898) (Nematoda: Metastrongylidae) can be found in the city of Guaratinguetá. It was found A. fulica wit...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652010000400009
更新日期:2010-07-01 00:00:00
abstract::The aim of this study was to evaluate the effects of the protein-calorie malnutrition in BALB/c isogenic mice infected with Lacazia loboi, employing nutritional and histopathological parameters. Four groups were composed: G1: inoculated with restricted diet, G2: not inoculated with restricted diet, G3: inoculated with...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652015000500009
更新日期:2015-09-01 00:00:00
abstract:INTRODUCTION:The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. METHODS:This is a study on the genetic profiles of 39 nosocomial clinical i...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652013000600003
更新日期:2013-11-01 00:00:00
abstract::The prevalence of thermotolerant Campylobacter in diarrhoeic and healthy children as well as in dogs, hens, ducks and pigeons was determined in Southern Chile. Campylobacter were found in 34.5% of the faecal samples examined. The isolation rate of Campylobacter in diarrhoeic and healthy children was 16.3% and 6.4% res...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000500007
更新日期:1994-09-01 00:00:00