Molecular typing of Candida albicans isolates from hospitalized patients.

Abstract:

INTRODUCTION:The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. METHODS:This is a study on the genetic profiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. RESULTS:RAPD provided 10 and 11 different profiles with values for S(AB) of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. CONCLUSIONS:Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans.

authors

Bonfim-Mendonça Pde S,Fiorini A,Shinobu-Mesquita CS,Baeza LC,Fernandez MA,Svidzinski TI

doi

10.1590/S0036-46652013000600003

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

385-91

issue

6

eissn

0036-4665

issn

1678-9946

pii

S0036-46652013000600385

journal_volume

55

pub_type

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