[Ultramicro-ELISA for the detection of IgM antibodies anti M. leprae].

Abstract:

:The availability of an ultramicroanalytic system (SUMA) and species-specific antigen of M. leprae obtained by chemical synthesis, have made possible the standardization and validation of an ultramicroELISA assay for detecting specific human IgM antibodies to this mycobacterium. The specificity of this test to demonstrate the infection with M. leprae was corroborated through a screening of 433 blood bank serum samples and other 265 from different groups (100, control group, 50 tuberculosis patients, 65 leprosy patients, 50 from household). The results obtained in the additional study of 140 household sero showed a high correlation (r = 0.98) with the conventional microELISA method. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.

authors

Laferte J,Abreu EG,Robaina R,Verez V

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

491-5

issue

6

eissn

0036-4665

issn

1678-9946

journal_volume

33

pub_type

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