Abstract:
:Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 +/- 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Argañaraz ER,Hubbard GB,Ramos LA,Ford AL,Nitz N,Leland MM,Vandeberg JL,Teixeira ARdoi
10.1590/s0036-46652001000500007subject
Has Abstractpub_date
2001-09-01 00:00:00pages
271-6issue
5eissn
0036-4665issn
1678-9946pii
S0036-46652001000500007journal_volume
43pub_type
杂志文章abstract::The municipality of Cantagalo is an area with sustained transmission of American Cutaneous Leishmaniasis (ACL). Monthly sand fly collections were performed for three years (June 2012 - May 2015) using a CDC light trap. A total of 3,310 specimens belonging to 12 species were trapped: Nyssomyia intermedia, Nyssomyia whi...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201658050
更新日期:2016-07-11 00:00:00
abstract::In order to contribute to a better understanding of the forms of acquisition of hepatitis C virus (HCV) in Brazil, with special emphasis on sexual transmission, we determined the presence of HCV infection in regular partners and in non-sexual home communicants of blood donors seen at Fundação Pró-Sangue Hemocentro de ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652001000300003
更新日期:2001-05-01 00:00:00
abstract::The authors report two cases of adrenal insufficiency secondary to infiltration of the adrenal glands by Paracoccidioides brasiliensis. The first patient had been treated for a chronic multifocal form of paracoccidiodomycosis 11 years ago. The diagnosis of the mycosis was done simultaneous with that of the adrenal ins...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652009000100008
更新日期:2009-01-01 00:00:00
abstract::Hand-foot-and-mouth disease (HFMD) is becoming one of the extremely common airborne and contact transmission diseases in Guangzhou, southern China, leading public health authorities to be concerned about its increased incidence. In this study, it was used an ecological study plus the negative binomial regression to id...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652014000600014
更新日期:2014-11-01 00:00:00
abstract::We all hope that biotechnology will answer some social and economical unavoidable requirements of the modern life. It is necessary to improve agriculture production, food abundance and health quality in a sustainable development. It is indeed a hard task to keep the progress on taking into account the rational use of ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 历史文章,杂志文章
doi:10.1590/s0036-46652001000400006
更新日期:2001-07-01 00:00:00
abstract::The frequency and class of immediate-type hypersensitivity manifestations were studied in 494 snakebitten and scorpion stung patients who were treated with intravenous injections of antivenom sera. These patients were admitted to HC-FMRPUSP from 1983 to 1988. The effectiveness of a hypersensitivity skin test was also ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::Between April 2003 and May 2009 phlebotomine sandflies were collected in Parque do Sabiá complex, Uberlândia municipality, Minas Gerais State, Brazil, using CDC and Shannon traps. The objective was to associate the sandfly species captured to the risk of the transmission of leishmaniasis in the municipality. The 126 c...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000500003
更新日期:2011-09-01 00:00:00
abstract::Giardia duodenalis isolates from asymptomatic or symptomatic patients and from animals present similarities and differences in the protein composition, antigenic profile, pattern of proteases and isoenzymes, as well as in nucleic acids analysis. In the present overview, these differences and similarities are reviewed ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46651999000100009
更新日期:1999-01-01 00:00:00
abstract::Recent human herpesvirus 6 (HHV-6) infection was detected in cases of exanthem subitum (ES) involving four children, aged 10 to 24 months, between April and August 1994, in Belém, Brazil. By using the indirect immunofluorescence antibody assay (IFA), significant increases (at least eight times) in antibody concentrati...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651995000600003
更新日期:1995-11-01 00:00:00
abstract::The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH respo...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651992000500003
更新日期:1992-09-01 00:00:00
abstract::A 25-year-old male without prior co-morbidities was admitted to hospital with Fusobacterium necrophorum bacteremia, where he was found to have liver and splenic abscesses. Further evaluation with echocardiography revealed a bicuspid aortic valve with severe insufficiency and a 1.68 x 0.86 cm vegetation. The patient re...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46652011000300009
更新日期:2011-05-01 00:00:00
abstract::Patients infected with the Human Immunodeficiency Virus (HIV) often have opportunistic infections, among which strongyloidiasis and coccidiosis are the most common parasitic infections that aggravate their health status. This study examined the prevalence of intestinal parasites, particularly of Strongyloides stercora...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201860013
更新日期:2018-03-08 00:00:00
abstract::A group of 16 patients with endemic pemphigus foliaceus under corticotherapy and still showing erythematous, papulous, verrucous, in general hyperpigmented lesions, which were characterized as cortico therapy resistant lesions, were studied. Such study was made through anatomopathology and direct immunofluorescence (D...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::Haptoglobin assay, a highly sensitive method to detect intravascular hemolysis was carried out in the sera of 19 patients referred to Hospital Vital Brazil with the cutaneous form of loxoscelism in order to investigate the occurrence of mild intravascular hemolysis. Data from this series did not show decreased levels ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000200009
更新日期:1994-03-01 00:00:00
abstract::Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and serodemes analysis. Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated cl...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651991000500002
更新日期:1991-09-01 00:00:00
abstract::The snail density, levels of infection and the monthly production of Schistosoma mansoni cercariae by Biomphalaria glabrata were determined in a focus of Barreiro de Baixo (Belo Horizonte, MG, Brazil). During a period of 38 months (1984 to 1987) 5,366 snails were collected of which 324 (6.03%) were infected with S. ma...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000600002
更新日期:1994-11-01 00:00:00
abstract::Numerous pulmonary schistosome egg granulomas were present in mice submitted to partial portal vein ligation (Warren's model). The granulomas were characterized by cellular aggregations formed within alveolar tissue. Main cellular types were macrophages (epithelioid cells), eosinophils, plasma cells and lymphocytes. T...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651993000500007
更新日期:1993-09-01 00:00:00
abstract::The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of poly...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652014000100009
更新日期:2014-01-01 00:00:00
abstract::A clinical case of cerebral infection by parenchymal racemose cysticercus, diagnosed by means of morphological characteristics in a patient of Valdivia city is described. The rare frequency of parenchymal location of racemose cysticercus as well as its differential diagnosis with other larval stages of cestodes that d...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651991000300011
更新日期:1991-05-01 00:00:00
abstract::This case report describes the findings of a 18 year-old black male from Bahia, a Northeastern state in Brazil, with the sickle cell trait, who developed bilateral hyphema and vitreous hemorrhage with blindness in the course of leptospirosis. The patient started to complain of blurred vision four days after the start ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652000000500009
更新日期:2000-09-01 00:00:00
abstract::An epidemiological survey with histoplasmin skin test was performed in 392 poultry farmers and 265 workers considered without occupational risk of exposition to Histoplasma capsulatum, etiologic agent of histoplasmosis. The results were positive in 28.8% and 13.2% in both groups respectively. Statistically, there was ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::Physical examination of nails was carried out in 210 elderly patients and nail scrapings were obtained from onychomycosis suggested lesions in order to determine their causative agents, incidence and clinical characteristics. Diagnostic was confirmed by the isolation of the agents from 74 patients, mainly from toe-nai...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651993000300001
更新日期:1993-05-01 00:00:00
abstract::A retrospective study was conducted in nine patients with rabies admitted to a hospital of Fortaleza, Brazil. Autopsy was performed in all cases. The ages ranged from three to 81 years and six were males. They all were bitten by dogs. The time between the accident and the hospital admission ranged from 20 to 120 days ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652005000600002
更新日期:2005-11-01 00:00:00
abstract::The development of small ultrasonographic equipment has permitted to incorporate this technique to the methods of early detection of Human Hydatidosis. 689 echographies were carried out in this experience, and 5.51% of compatible images in the whole population, and 12.24% in groups of risk (those living together with ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000400010
更新日期:1989-07-01 00:00:00
abstract::In human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, three mixed visceral...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000200001
更新日期:2011-03-01 00:00:00
abstract::The aim of this research was to evaluate the prevalence of Staphylococcus spp. and S. aureus in the odontological clinic environment (air), their production of beta-lactamase and antibacterial susceptibility to the major antibiotics utilized in medical particle. During 12 months of samples collect were isolated 9775 C...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652005000100004
更新日期:2005-01-01 00:00:00
abstract::In Brazil, the main etiologic agent of Leishmaniasis that frequently presents with mucosal involvement belongs to the Viannia subgenus. The therapeutic conduct in this disease depends on the parasitological diagnosis, and classical methods are restricted in identifying the agent. In this paper we describe a polymerase...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652004000600007
更新日期:2004-11-01 00:00:00
abstract::The authors reported one case of eumycotic mycetoma due to Madurella grisea (black grains) occurred on the right foot of the patient studied. The structure, microscopic morphology and therapeutic evolution are also studied and reported. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000300010
更新日期:1989-05-01 00:00:00
abstract::After the introduction of the rotavirus vaccine, the number of rotavirus-associated deaths and the predicted annual rotavirus detection rate had slightly declined worldwide. Taking in account that in Colombia, Rotarix vaccine was introduced in 2009, the purpose of this study was to evaluate the presence of rotavirus A...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201961034
更新日期:2019-07-01 00:00:00
abstract::Twenty seven cases of neurocysticercosis, with clinical picture of acute meningitis, are described. Twenty (74.1%) patients are male; the age was 4 to 42 years (23.6 +/- 11.7 years). The etiologic diagnosis was defined by the complement fixation test (Weinberg) and/or enzyme-linked immunosorbent assay (ELISA) for cyst...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00