Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons.

Abstract:

:Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 +/- 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony.

authors

Argañaraz ER,Hubbard GB,Ramos LA,Ford AL,Nitz N,Leland MM,Vandeberg JL,Teixeira AR

doi

10.1590/s0036-46652001000500007

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

271-6

issue

5

eissn

0036-4665

issn

1678-9946

pii

S0036-46652001000500007

journal_volume

43

pub_type

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