Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus.

Abstract:

:In Brazil, the main etiologic agent of Leishmaniasis that frequently presents with mucosal involvement belongs to the Viannia subgenus. The therapeutic conduct in this disease depends on the parasitological diagnosis, and classical methods are restricted in identifying the agent. In this paper we describe a polymerase chain reaction (PCR), which uses primers designed from mini-exons repetitive sequences. The PCR amplifies a 177bp fragment that can distinguish (Viannia) from (Leishmania) subgenus. This test could be a useful diagnostic tool.

authors

Paiva BR,Passos LN,Falqueto A,Malafronte Rdos S,Andrade HF Jr

doi

10.1590/s0036-46652004000600007

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

335-8

issue

6

eissn

0036-4665

issn

1678-9946

pii

S0036-46652004000600007

journal_volume

46

pub_type

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