Abstract:
:Formalin-killed promastigotes (FKP) of Leishmania major, in combination with Montanide ISA 720 (MISA), BCG or alum were used in vaccination of an inbred murine model against cutaneous leishmaniasis (CL). Significant and specific increases in anti-FKP IgG responses were detected for both alum-FKP and BCG-FKP compared to MISA-FKP (p < 0.001). Significant increases in splenic lymphocyte recall proliferation was obtained in the MISA-FKP vaccinated mice compared to alum-FKP or BCG-FKP vaccinated groups (p < 0.01). The highest interferon-gamma responses were observed in the BCG-FKP group followed by the MISA-FKP while the alum-FKP gave the least responses. Significantly reduced lesion sizes were obtained in the MISA-FKP group compared to the BCG/alum adjuvants-FKP vaccinated groups. Although the BCG-FKP group showed the highest IFN-gamma responses, it failed to control cutaneous lesions. Significant reductions in parasite numbers were observed in the MISA-FKP and BCG-FKP vaccinated groups (p < 0.001). There was a good correlation between parasite burden and IFN-gamma level indicating IFN-gamma response as a sensitive parameter of the immune status. In conclusion, MISA-FKP is the most efficacious vaccine formulation against murine cutaneous leishmaniasis.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Mutiso JM,Macharia JC,Mutisya RM,Taracha Edoi
10.1590/s0036-46652010000200006subject
Has Abstractpub_date
2010-03-01 00:00:00pages
95-100issue
2eissn
0036-4665issn
1678-9946pii
S0036-46652010000200006journal_volume
52pub_type
杂志文章abstract::The aim of this study was to describe the epidemiological profile of snakebite accidents reported by the toxicological assistance center in Fortaleza, Ceará, Brazil. Database information on snakebite accidents was analyzed regarding the period from January 2003 to December 2011. A total of 1063 cases were found. The a...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652013000500009
更新日期:2013-09-01 00:00:00
abstract::A sedimentation technique is described, in which a fecal suspension is placed on top of an aqueous sucrose solution of specific gravity 1.015 g/cm3. Using 100 by 15 mm test tubes, duplicate gravity sedimentation experiments were made using homogenized fecal suspensions (single-columns) and fecal suspensions placed on ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651992000300013
更新日期:1992-05-01 00:00:00
abstract::The aim of the present work was to determine the prevalence of IgG and IgM anti-Toxoplasma gondii antibodies and the factors associated to the infection in pregnant women attended in Basic Health Units in Rolândia, Paraná, Brazil. The sample was divided in two groups: group I (320 pregnant women who were analyzed from...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000400002
更新日期:2011-07-01 00:00:00
abstract::Intestinal parasitic infections are prevalent throughout many countries. This study aimed to determine the prevalence of intestinal parasite carriers among 21,347 expatriate workers, including food handlers and housemaids attending the public health center laboratory in Sharjah, UAE. Stool sample collection was perfor...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201759082
更新日期:2017-12-21 00:00:00
abstract::Neurocysticercosis (NC), the presence of Taenia solium metacestodes in tissues, is the most frequent and severe parasitic infection of the central nervous system. We investigated the presence of total IgE by an automated chemiluminescence assay in 53 paired cerebrospinal fluid (CSF) and serum samples from patients wit...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652000000200002
更新日期:2000-03-01 00:00:00
abstract::The pathogenesis of chronic chagasic myocarditis remains incompletely understood. Several hypotheses have been proposed: (1) direct tissue destruction by Trypanosoma cruzi; (2) neurogenic theory; (3) anti-heart immune reactions; and (4) microvascular disease. We present herein a dynamic alternative hypothesis. We beli...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46651992000600015
更新日期:1992-11-01 00:00:00
abstract::Dengue is a viral disease, caused by an arbovirus of the genus Flavivirus. In Brazil, its incidence rate is high with a broad clinical spectrum. This report discusses a rare case of dengue associated with cutaneous leukocytoclastic vasculitis and pericardial effusion with eminence of cardiac tamponade in a previously ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type:
doi:10.1590/S1678-9946202062101
更新日期:2020-12-18 00:00:00
abstract::This study presents the results of T. mentagrophytes inoculation in the cheek pouch of the hamster, an immunologically privileged site. Forty two animals were used: 21 inoculated with 10(6) fungi in the cheek pouch (group 1) and 21 inoculated initially with 10(6) fungi in the foot pad and 15 days later in the cheek po...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652001000100006
更新日期:2001-01-01 00:00:00
abstract::Forty children with a diagnosis of Visceral Toxocariasis were evaluated prospectively from February 1982 to June 1989. Diagnosis was established by clinical, laboratory and serological (ELISA - ES Toxocara canis antigen) evaluations. A great clinical polymorphism was found in our patients, ranging from unspecific or a...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000100004
更新日期:1994-01-01 00:00:00
abstract::The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651998000200003
更新日期:1998-03-01 00:00:00
abstract::Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This di...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652003000600003
更新日期:2003-11-01 00:00:00
abstract::A comparative study of the "in vitro" susceptibility of 24 Cryptococcus strains to 5 antifungal drugs (amphotericin B, 5 fluorocytosine, miconazole, itraconazole and ketoconazole), was carried out. These strains were grouped according to species, varieties and isolation's origins. The minimum inhibitory concentration ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000500009
更新日期:1989-09-01 00:00:00
abstract::Pathogenic Yersinia pestis isolates were collected during a plague outbreak at the Paraiba State in 1986. The Y. pestis isolates were investigated for the presence of virulence-associated factors and plasmid content. All strains analysed were proficient in the expression of the VW and fraction 1 antigens, pigment adso...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000500001
更新日期:1989-09-01 00:00:00
abstract::The municipality of Cantagalo is an area with sustained transmission of American Cutaneous Leishmaniasis (ACL). Monthly sand fly collections were performed for three years (June 2012 - May 2015) using a CDC light trap. A total of 3,310 specimens belonging to 12 species were trapped: Nyssomyia intermedia, Nyssomyia whi...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201658050
更新日期:2016-07-11 00:00:00
abstract::Canine visceral leishmaniasis (CVL) is recognizable by characteristic signs of disease and is highly lethal. The infection, however, may be quite inapparent in some seropositive dogs, and this has raised the polemic question as to whether or not such animals can be a source of infection for Lutzomyia longipalpis, the ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652010000500007
更新日期:2010-09-01 00:00:00
abstract::American Tegumentary Leishmaniasis (ATL) is endemic in the municipality of Montes Claros, Minas Gerais State. The use of geotechnology such as spatial statistics and remote sensing has contributed to a better understanding of the eco-epidemiology of diseases, and consequently a better definition of control strategies....
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946202163002
更新日期:2021-01-20 00:00:00
abstract:BACKGROUND:Pemphigus vulgaris (PV) is an autoimmune disease characterized by blistering of the skin and mucosa, which develops due to the interaction between predisposing genetic and environmental factors. Infections caused by members of the Herpesviridae family have been suggested as a possible triggering factor for P...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46652011000200010
更新日期:2011-03-01 00:00:00
abstract::The administration of viable Bifidobacterium animalis was tested to induce resistance against Strongyloides venezuelensis infection in mice. Effects on parasite burden, worm length, egg output, and intestinal mucosal histology were evaluated. The oral administration of B. animalis, strain 04450B, starting 14 days befo...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652014000200003
更新日期:2014-03-01 00:00:00
abstract::Three patients with the diagnosis of subacute juvenile paracoccidioidomycosis who, at the time of their first visit, had no signs or symptoms of lung involvement were studied. Initially the diagnosis was confirmed by the observation of P. brasiliensis in biopsy material obtained from clinically involved lymphadenopath...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000100004
更新日期:1989-01-01 00:00:00
abstract::Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651996000500009
更新日期:1996-09-01 00:00:00
abstract::Three autochthonous cases of cutaneous-ganglionar rickettsiosis transmitted by dogs ticks (Amblyomma maculatum in one of them) are reported. Two of the three cases were undoubtely produced by Rickettsia conorii according to the results of the specific indirect immunofluorescence technique IEF-IgM anti R. conorii. A th...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000500001
更新日期:1990-09-01 00:00:00
abstract::The specificity and sensitivity of the enzyme immunoassay (EIA), presently used in South America areas where hydatidosis caused by Echinococcus granulosus is endemic, was compared to two alternative EIA. One of these uses an hydatid antigen of different preparation and the other vesicular fluid of Taenia crassiceps ci...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651993000200007
更新日期:1993-03-01 00:00:00
abstract::The Mato Grosso do Sul State (MS) has the second-largest indigenous population and the highest incidence rates of TB among indigenous people in Brazil. However, little is known about the risk factors associated with active TB in indigenous people in the region, especially regarding socioeconomic factors. The aim of th...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201860063
更新日期:2018-10-25 00:00:00
abstract::The objective of this study was to verify the occurrence of intestinal parasites in 3 to 6-year-old children from daycare centers maintained by the municipal government of Belo Horizonte, Minas Gerais, Brazil. Coproparasitological tests performed in 472 children have shown that 24.6% of them had some type of parasites...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652008000100013
更新日期:2008-01-01 00:00:00
abstract::The response to interferon treatment in chronic hepatitis NANB/C has usually been classified as complete, partial or absent, according to the behavior of serum alanine aminotransferase (ALT). However, a more detailed observation of the enzymatic activity has shown that the patterns may be more complex. The aim of this...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651995000300010
更新日期:1995-05-01 00:00:00
abstract::Among the 424 serum samples examined, the prevalence of hepatitis virus infection turned out to be 89.6%, with 15.6% of HBsAg positivity. Some of the samples belonged to an afferent population and some other to workers of a West Africa rural hospital (Pop. Rep. of Benin). 27.3% of the positive subjects presented activ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000100006
更新日期:1990-01-01 00:00:00
abstract::The aim of this article is to present an investigation of cure rate, after long follow up, of specific chemotherapy with benznidazole in patients with both acute and chronic Chagas disease, applying quantitative conventional serological tests as the base of the criterion of cure. Twenty one patients with the acute for...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 临床试验,杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::In the study of conjunctivitis outbreaks occurring from September 1994 to September 1996 in the region of Ribeirão Preto, conjunctival exudates of 92 patients were cultivated in Instituto Adolfo Lutz Laboratory I, Ribeirão Preto. Most cases occurred in the age range 2-7 years. The etiological agents which were most fr...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651998000100002
更新日期:1998-01-01 00:00:00
abstract::Surfacen® is an exogenous natural lung surfactant, composed by phospholipids and hydrophobic proteins, which is applied successfully in Newborn Respiratory Distress Syndrome. In this paper, in vitro activity of Surfacen® against Leishmania amazonensis is described. The product showed activity against the amastigote fo...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000400012
更新日期:2011-07-01 00:00:00
abstract::The objective of the present study was to estimate the prevalence of herpes simplex virus type 2 (HSV 2) antibodies in child bearing women of 2 Brazilian populations with different socioeconomic status and to determine the risk of neonatal HSV exposure by means of maternal cultures at the onset of labor. The study was...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,多中心研究
doi:10.1590/s0036-46651993000300011
更新日期:1993-05-01 00:00:00