Abstract:
:The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected patients at acute and chronic phase. There was an overall coincidence of molecular mass among antigens of tachyzoites and tissue cysts recognized by these sera, however, they appear not to be the same molecules. The response against tissue cysts starts early during acute infection, and the reactivity of antibodies is strong against a wide range of antigens. Six bands (between 82 and 151 kDa) were exclusively recognized by chronic phase sera but only the 132 kDa band was positive in more than 50% of the sera analysed. A mixture of these antigens could be used to discriminate between the two infection phases. The most important antigens recognized by the acute and the chronic phase sera were 4 clusters in the ranges 20-24 kDa, 34-39 kDa, 58-80 kDa and 105-130 kDa as well as two additional antigens of 18 and 29 kDa. Both accidentally infected patients and some of the naturally infected patients showed a weak specific response against tissue cyst antigens.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Villavedra M,Carol H,Nieto Adoi
10.1590/s0036-46651998000200003subject
Has Abstractpub_date
1998-03-01 00:00:00pages
77-84issue
2eissn
0036-4665issn
1678-9946journal_volume
40pub_type
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journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
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