A simple PCR-based procedure for plague diagnosis.

Abstract:

:Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.

authors

Leal NC,Abath FG,Alves LC,de Almeida AM

doi

10.1590/s0036-46651996000500009

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

371-3

issue

5

eissn

0036-4665

issn

1678-9946

journal_volume

38

pub_type

杂志文章
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