Abstract:
:In an attempt to find a better T. cruzi antigen and possible immunological markers for the diagnosis of different clinical forms of Chagas'disease, amastigote and trypomastigote antigens obtained from immunosuppressed mice infected with T. cruzi (Y strain) were assessed in comparison with conventional epimastigote antigens. A total of 506 serum samples from patients with acute and with chronic (indeterminate, cardiac and digestive) forms, from nonchagasic infections, and from healthy individuals were assayed in immunofluorescence (IF) tests, to search for IgG, IgM and IgA antibodies. Amastigote proved to be the most convenient antigen for our purposes, providing higher relative efficiency indexes of 0.946, 0.871 and 0.914 for IgG, IgM and IgA IF tests, respectively. Anti-amastigote antibodies presented higher geometric mean titers (GMT) than anti-trypomastigote and anti-epimastigote. Anti-amastigote IgG antibodies were found in all forms of Chagas'disease, and predominantly IgA antibodies, in chronic digestive and in acute forms, as well as IgM antibodies, in latter forms. Thus, tests with amastigote antigen could be helpful for screening chagasic infections in blood banks. Practical and economical aspects in obtaining amastigotes as here described speak in favour of its use in developing countries, since those from other sources require more complex system of substruction, specialized personnel or equipment.
journal_name
Rev Inst Med Trop Sao Paulojournal_title
Revista do Instituto de Medicina Tropical de Sao Pauloauthors
Primavera KS,Umezawa ES,Peres BA,Camargo ME,Hoshino-Shimizu Sdoi
10.1590/s0036-46651990000300005subject
Has Abstractpub_date
1990-05-01 00:00:00pages
172-80issue
3eissn
0036-4665issn
1678-9946journal_volume
32pub_type
杂志文章abstract::[This corrects the article doi: 10.1590/s1678-9946201961041]. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 已发布勘误
doi:10.1590/S1678-9946201961041err
更新日期:2019-09-12 00:00:00
abstract::A 37-year-old male patient, Fitzpatrick skin phototype IV, a student living in Belem, Amazon region, in 2015 had a confirmed diagnosis of acquired immunodeficiency virus (HIV) infection, but did not initiate antiretroviral treatment at his own option. Three years after the diagnosis, erythematous maculae appeared on t...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type:
doi:10.1590/S1678-9946202062002
更新日期:2020-01-17 00:00:00
abstract::This paper reports the findings of a survey for intestinal parasites among the Xavánte Indians from Central Brazil. A. lumbricoides (25.0%) and hookworms (33.6%) were the two most common helminths; E. histolytica complex (7.8%) and G. lamblia (8.6%) the most common protozoans. The majority (58.5%) of positive individu...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651995000200009
更新日期:1995-03-01 00:00:00
abstract::Hand-foot-and-mouth disease (HFMD) is becoming one of the extremely common airborne and contact transmission diseases in Guangzhou, southern China, leading public health authorities to be concerned about its increased incidence. In this study, it was used an ecological study plus the negative binomial regression to id...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652014000600014
更新日期:2014-11-01 00:00:00
abstract::Fasciolosis is a widespread parasitosis of farm live-stock in many developing countries. For this reason, it is necessary to search for new substances against parasitic diseases caused by flukes. Indeed, a wide variety of terrestrial plants have been subjected to chemical and pharmacological screening in order to disc...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652015000600003
更新日期:2015-12-01 00:00:00
abstract::The aim of this article is to present an investigation of cure rate, after long follow up, of specific chemotherapy with benznidazole in patients with both acute and chronic Chagas disease, applying quantitative conventional serological tests as the base of the criterion of cure. Twenty one patients with the acute for...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 临床试验,杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::In this study, Leishmania species were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia tri-border was also investigated. By PCR, the DNA of Leishmania was...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S0036-46652015000400012
更新日期:2015-07-01 00:00:00
abstract::The Authors report a case of paracoccidioidomycosis in a Surui Indian patient from the state of Rondonia, Brazilian Amazon. The subject is an adult male, having been diagnosed on the basis of mycologic, serologic, and radiographic exams. The prescribed therapy was sulphamethoxazole 800 mg associated with trimethoprim ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::Thirty eight paralysis cases classified as Guillain-Barré syndrome (GBS) in Brazil were analysed. In all these cases Sabin-related poliovirus vaccine strains were isolated. In most of the cases the last vaccine dose was given months or years before the onset of GBS, suggesting a persistent infection or the transmissio...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651996000100010
更新日期:1996-01-01 00:00:00
abstract::The authors reported one case of eumycotic mycetoma due to Madurella grisea (black grains) occurred on the right foot of the patient studied. The structure, microscopic morphology and therapeutic evolution are also studied and reported. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651989000300010
更新日期:1989-05-01 00:00:00
abstract:UNLABELLED:Hepatocellular carcinoma (HCC) is an important type of cancer etiologically related to some viruses, chemical carcinogens and other host or environmental factors associated to chronic liver injury in humans. The tumor suppressor gene p53 is mutated in highly variable levels (0-52%) of HCC in different countr...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652004000100005
更新日期:2004-01-01 00:00:00
abstract::Although Candida albicans is the main cause of fungal esophagitis, other species such as C. tropicalis, C. krusei and C. stellatoidea have also been implicated. Several studies have identified risk factors for C. albicans esophagitis. However, data for non-C. albicans species is still sparse. The aim of this study was...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652008000500002
更新日期:2008-09-01 00:00:00
abstract::A significant number of Brazilian gestational-age women are still not tested for HIV, representing a high risk of transmission to their newborns. The current study sought to identify the number of pregnant women with no previous testing or undocumented for HIV referred to the Gynecology and Obstetrics Department of a ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652009000500007
更新日期:2009-09-01 00:00:00
abstract::Pleural effusions are rare events accompanying histoplasmosis, and in general they are associated with acute forms of the disease. It is described here a case of histoplasmoma associated with pleuritic pain and pleural effusion. ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651990000500012
更新日期:1990-09-01 00:00:00
abstract::Therapy of coccidioidomycosis continues to evolve. For primary pulmonary disease, antifungal therapy is frequently not required while prolonged courses of antifungals are generally needed for those in whom extrathoracic disseminated has occurred. Intravenous amphotericin B should be reserved for those with severe dise...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/S0036-46652015000700010
更新日期:2015-09-01 00:00:00
abstract::An E.L.I.S.A. test for antibody detection, with an exo-antigen of Coccidioides immitis was standardized in 67 humans sera diluted in 1/1000, 1/2000, 1/4000 and 1/8000. Eighteen sera from mycologically proved cases of coccidioidomycosis were studied: 5 were negative and 13 were positive in some dilutions. 3/26 sera of ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651991000400007
更新日期:1991-07-01 00:00:00
abstract::In human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, three mixed visceral...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652011000200001
更新日期:2011-03-01 00:00:00
abstract::This work aimed to assess pathogenic potential and clonal relatedness of Aeromonas sp. and Vibrio cholerae isolates recovered during a diarrhea outbreak in Brazil. Clinical and environmental isolates were investigated for the presence of known pathogenic genes and clonal relatedness was assessed by intergenic spacer r...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652012000600001
更新日期:2012-12-01 00:00:00
abstract::This study evaluated the whole blood immunochromatographic card test (ICT card test) in a survey performed in Northeastern Brazil. 625 people were examined by the thick blood film (TBF) and ICT card test. Residents of a non-endemic area were also tested by the whole blood card test and Og4C3. The sensitivity of the IC...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652003000300002
更新日期:2003-05-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) is an important factor contributing to morbidity and mortality in patients co-infected with HIV and HCV. In addition, liver biopsy is an important tool in the clinical management of these patients. Although liver biopsy is controversial, it is recommended for all patients. Data regard...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652008000400005
更新日期:2008-07-01 00:00:00
abstract::The 4-year study (1987-1990) covered the major clinical-epidemiological characteristics of pneumonia in children as diagnosed at the emergency service of the Children's Hospital, as well as etiologies, and factors involved in the most severe cases. Etiology was determined in 47.7% of the 541 pneumonia cases, involving...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000300010
更新日期:1994-05-01 00:00:00
abstract::A series of already published and unpublished seroepidemiological surveys for toxoplasmosis, carried out in Chile in 1982-1994, is reviewed, expanded and analyzed. The surveys included 76,317 apparently healthy individuals of different ages (0.57% of the country's total population), from 309 urban and rural-periurban ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/s0036-46651996000600008
更新日期:1996-11-01 00:00:00
abstract::Forty children with a diagnosis of Visceral Toxocariasis were evaluated prospectively from February 1982 to June 1989. Diagnosis was established by clinical, laboratory and serological (ELISA - ES Toxocara canis antigen) evaluations. A great clinical polymorphism was found in our patients, ranging from unspecific or a...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46651994000100004
更新日期:1994-01-01 00:00:00
abstract::Systemic fungal infections are responsible for high mortality rates. Several species of fungi may be involved, but Candida spp. is the most prevalent. Simvastatin is used to lower cholesterol and also exhibits antifungal action. The aim of this study was to evaluate the synergistic action of simvastatin with fluconazo...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652012000400003
更新日期:2012-08-01 00:00:00
abstract::The aim of this review is to show the significant role of HIF-1alpha in inflammatory and infectious diseases. Hypoxia is a physiological characteristic of a wide range of diseases from cancer to infection. Cellular hypoxia is sensed by oxygen-sensitive hydrolase enzymes, which control the protein stability of hypoxia-...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章,评审
doi:10.1590/S1678-9946201759092
更新日期:2017-12-21 00:00:00
abstract::This study aims to assess the association between schistosomiasis and hookworm infection with hemoglobin levels of schoolchildren in northern Mozambique. Through a cross-sectional survey, 1,015 children from five to 12 years old in the provinces of Nampula, Cabo Delgado and Niassa were studied. Hookworm infection and ...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652014000300007
更新日期:2014-05-01 00:00:00
abstract::The dissemination of the giant African snail Achatina fulica in several countries has triggered a great number of studies on the mollusk, including those on zoonoses related to health in humans. The current research is a scientific survey on articles published in four databases, namely, PubMed, Bireme, Scielo and Lila...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/S1678-9946201759015
更新日期:2017-04-13 00:00:00
abstract::It is essential that healthcare providers are familiar with the full spectrum of clinical presentations of syphilis. A rare manifestation of secondary syphilis is the corymbiform (or corymbose) arrangement, in which a central greater papule is surrounded by smaller satellite lesions. Very few reports of corymbiform sy...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type:
doi:10.1590/S1678-9946201860040
更新日期:2018-08-20 00:00:00
abstract::In late 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged as the cause of a cluster of pneumonia cases in China, and the corresponding disease was designated as Coronavirus Disease 2019 (COVID-19), spreading quickly around the world resulting in a pandemic. COVID-19 is associated with a se...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type:
doi:10.1590/S1678-9946202062063
更新日期:2020-09-04 00:00:00
abstract::Between April and October 2002, thirty fortnightly collections of oysters (Crassostrea rhizophorea) from a natural oyster bed at the Cocó River estuary in the Sabiaguaba region (Fortaleza, Ceará, Brazil) were carried out, aiming to isolate Aeromonas spp. strains. Oyster samples were submitted to the direct plating (DP...
journal_title:Revista do Instituto de Medicina Tropical de Sao Paulo
pub_type: 杂志文章
doi:10.1590/s0036-46652006000300003
更新日期:2006-05-01 00:00:00