Abstract:
:The availability of fluorescent dyes and the advances in the optical systems for in vivo imaging have stimulated an increasing interest in developing new methodologies to study and quantify the biodistribution of labeled agents. However, despite these great achievements, we are facing significant challenges in determining if the observed fluorescence does correspond to the quantity of the dye in the tissues. In fact, although the far-red and near-infrared lights can propagate through several centimetres of tissue, they diffuse within a few millimetres as consequence of the elastic scattering of photons. In addition, when dye-labeled oligonucleotides form stable complex with cationic carriers, a large change in the fluorescence intensity of the dye is observed. Therefore, the measured fluorescence intensity is altered by the tissue heterogeneity and by the fluctuation of dye intensity. Hence, in this study a quantification strategy for fluorescence-labeled oligonucleotides was developed to solve these disadvantageous effects. Our results proved that upon efficient homogenization and dilution with chaotropic agents, such as guanidinium thiocyanate, it is possible to achieve a complete fluorescence intensity recovery. Furthermore, we demonstrated that this method has the advantage of good sensitivity and reproducibility, as well as easy handling of the tissue samples.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Munisso MC,Yamaoka Tdoi
10.1155/2014/196837subject
Has Abstractpub_date
2014-01-01 00:00:00pages
196837eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract::Understanding the impact of helminth infections on clinical malaria is useful for designing effective malaria control strategies. Plenty of epidemiological studies have been conducted to unravel the nature of interactions between Plasmodium and helminth infection. Careful broad summarization of the existing literature...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2016/3083568
更新日期:2016-01-01 00:00:00
abstract::The aim of this study was to compare the prevalence of different virulence factor (VF) genes in extended-spectrum beta-lactamase (ESBL) producing Escherichia coli strains isolated from the Baltic Sea region. A total of 432 strains of phenotypically ESBL positive E. coli were collected from 20 institutions located in E...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/427254
更新日期:2014-01-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder caused by selective loss of dopaminergic neurons in the substantia nigra pars compacta which leads to dysfunction of cerebral pathways critical for the control of movements. The diagnosis of PD is based on motor symptoms, such as bradykinesia, akinesia, muscular...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/519816
更新日期:2014-01-01 00:00:00
abstract::Cancer metastasis has been demonstrated as it is the culmination of a cascade of priming steps. Increasing evidence has shown that tumor-derived molecular components (TDMCs) are known as extra cellular vesicle and nonvesicle factors and serve as versatile intercellular communication vehicles which can mediate signalin...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2019/9258075
更新日期:2019-06-12 00:00:00
abstract::Background. Findings on the association between posttransplant anemia (PTA) and mortality in posttransplant patients are scarce. This study explored whether PTA shortly after kidney transplantation (KT) predicts mortality at up to 10 years' follow-up, stratified for chronic kidney disease (CKD) stages. Methods. PTA wa...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/6987240
更新日期:2017-01-01 00:00:00
abstract::The aim of the present study is to analyse the secondary bacterial infections in a large group of patients with seasonal influenza A and influenza A(H1N1) pdm09. Patients diagnosed with seasonal influenza A and influenza A(H1N1) pdm09 between 2005 and 2009 were enrolled in the study. Data was retrieved from medical re...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/376219
更新日期:2013-01-01 00:00:00
abstract::[This corrects the article DOI: 10.1155/2016/2643862.]. ...
journal_title:BioMed research international
pub_type: 已发布勘误
doi:10.1155/2017/5782619
更新日期:2017-01-01 00:00:00
abstract::Apc-mutated Pirc rats, spontaneously developing intestinal tumours, are resistant to 1,2-dimethylhydrazine- (DMH-) induced colon apoptosis. To understand this phenomenon, we analyzed the expression of genotoxic stress-related genes Mgmt, Gsta1, and Gstp1 in the colon of wt and Pirc rats in basal conditions and 24 h af...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/1310342
更新日期:2016-01-01 00:00:00
abstract:Aim:The aim of this study is to detect the prevalence of substance use among tenth-grade students; their thoughts, attitudes, behaviors, and tendencies towards substance use; and risk factors of substance use in tenth-grade students in general. Methods:This study is descriptive and cross-sectional conducted between Ap...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1407649
更新日期:2018-03-15 00:00:00
abstract::ELABELA (ELA) is considered to be implicated in the pathophysiology of preeclampsia (PE), since ELA-deficient mice exhibited PE-like symptoms and infusion of exogenous ELA normalized the gestational hypertension (GH) and proteinuria. However, no evidence show that circulating ELA is deficient in early placental develo...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/2051701
更新日期:2020-09-28 00:00:00
abstract::Robot-Assisted Rehabilitation (RAR) is relevant for treating patients affected by nervous system injuries (e.g., stroke and spinal cord injury). The accurate estimation of the joint angles of the patient limbs in RAR is critical to assess the patient improvement. The economical prevalent method to estimate the patient...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/2581924
更新日期:2016-01-01 00:00:00
abstract::Chronic hypoxia (CH) is characterized by long-term hypoxia that is associated with microvessel proliferation and basal membrane (BM) degradation in tissues. The IL-6/JAK2/STAT3/MMP-9 pathway has been described in a variety of human cancers and plays an essential role in microvessel proliferation and BM degradation. Th...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9204708
更新日期:2020-01-23 00:00:00
abstract::Metastasis is the leading cause of lung cancer-associated death. Downregulated expression of E-cadherin followed by epithelial-mesenchymal transition (EMT) is critical for metastasis initiation in lung cancer. BTBD7 plays essential roles in lung cancer metastasis, but the mechanisms remain unknown. This study aimed to...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/5937635
更新日期:2019-11-27 00:00:00
abstract::Embryology of normal web space creation and the genetics of syndactyly in humans and experimental animals are well described in the literature. In this review, the author offers a 3-step pathway of pathogenesis for syndactyly. The first step is initiated either by the overactivation of the WNT canonical pathway or the...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2019/9652649
更新日期:2019-09-15 00:00:00
abstract::Epithelial Cl(-) secretion plays important roles in water secretion preventing bacterial/viral infection and regulation of body fluid. We previously suggested that quercetin would be a useful compound for maintaining epithelial Cl(-) secretion at a moderate level irrespective of cAMP-induced stimulation. However, we n...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/902735
更新日期:2014-01-01 00:00:00
abstract::The AT-hook transcription factor, AKNA, is a nuclear protein that affects a few physiological and pathological processes including cancer. Here, we investigated the role of AKNA in gastric cancer (GC). By using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays, AKNA was found deregulat...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6726759
更新日期:2020-05-13 00:00:00
abstract::Tyrosol hydroxylating Pseudomonas strain was previously isolated from olive mill wastewaters-irrigated soil. In the present work, experimental design was used to study the bioconversion of tyrosol in laboratory fermenters aiming at the recovery of the highest yields of hydroxytyrosol. The effects of biocatalyst loadin...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/7390751
更新日期:2018-07-09 00:00:00
abstract::Legumes have developed a unique way to interact with bacteria: in addition to preventing infection from pathogenic bacteria like any other plant, legumes also developed a mutualistic symbiotic relationship with one gender of soil bacteria: rhizobium. This interaction leads to the development of a new root organ, the n...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/507946
更新日期:2014-01-01 00:00:00
abstract::The cyprinid genus Pteronotropis is endemic to southeastern Gulf of Mexico and Atlantic Ocean of North America. Never before has the genus been demonstrated to be monophyletic. We investigate both the phylogenetic relationships and the phylogeography of some species in the genus using mitochondrial ND2 sequences. In n...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/675260
更新日期:2015-01-01 00:00:00
abstract::The aim of this study was to trigger the expression of genes related to oocytes in putative ovarian stem cells scraped from the ovarian surface epithelium of women with premature ovarian failure and cultured in vitro in the presence of follicular fluid, rich in substances for oocyte growth and maturation. Ovarian surf...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/861460
更新日期:2013-01-01 00:00:00
abstract::Reactive oxygen species (ROS) damage macromolecules and cellular components in nearly all kinds of cells and often generate toxic intracellular byproducts. In this work, aldehyde generation derived from the Escherichia coli membrane oxidation as well as membrane fatty acid profiles, protein oxidation, and bacterial re...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/563756
更新日期:2013-01-01 00:00:00
abstract::A diffusion theory-based, all-physical ab initio protein folding simulation is described and applied. The model is based upon the drift and diffusion of protein substructures relative to one another in the multiple energy fields present. Without templates or statistical inputs, the simulations were run at physiologic ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/583045
更新日期:2013-01-01 00:00:00
abstract::To further understand the potential expression relationships of miRNAs in miRNA gene clusters and gene families, a global analysis was performed in 4 paired tumor (breast cancer) and adjacent normal tissue samples using deep sequencing datasets. The compositions of miRNA gene clusters and families are not random, and ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/782490
更新日期:2014-01-01 00:00:00
abstract:Introduction:Baseline neutrophil-to-lymphocyte ratio (NLR) has been repeatedly reported as a significant prognostic factor in advanced cancer patients. We explored whether changes in NLR may predict outcome of advanced cancer patients enrolled into phase 1 trials and treated with PD-1/PD-L1 inhibitors. Patients and Me...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/1506824
更新日期:2017-01-01 00:00:00
abstract::Several lines of evidence indicate that amyloid β (Aβ), particularly Aβ oligomers (AβOs), plays a causative role in Alzheimer's disease. However, the mechanisms underlying the action of an anti-AβO antibody to clarify the toxic action of AβOs remain elusive. Here, we showed that the anti-AβO antibody (monoclonal 72D9)...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/984041
更新日期:2013-01-01 00:00:00
abstract::Astrocytic impairment is a pathologic feature of neuromyelitis optica spectrum disorder (NMOSD). S100B and glial fibrillary acidic protein (GFAP) are the two most commonly used astrocytic markers. The aim of this study was to evaluate whether CSF-S100B could serve as a marker of NMOSD. We enrolled 49 NMOSD patients [2...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2018/5381239
更新日期:2018-10-22 00:00:00
abstract::Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune syste...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/143504
更新日期:2015-01-01 00:00:00
abstract::Exposure to microgravity conditions causes cardiovascular deconditioning in astronauts during spaceflight. Until now, no specific drugs are available for countermeasure, since the underlying mechanism is largely unknown. Endothelial cells (ECs) and smooth muscle cells (SMCs) play key roles in various vascular function...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/413708
更新日期:2014-01-01 00:00:00
abstract::This study aims to ascertain whether cytokines in the aqueous humor can predict macular edema (ME) in diabetic patients following uncomplicated phacoemulsification cataract surgery. Undiluted aqueous humor samples were obtained from 136 consecutive type 2 diabetic patients who underwent cataract surgery. The concentra...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/126984
更新日期:2015-01-01 00:00:00
abstract::Among other diseases characterized by the onset of cachexia, congestive heart failure takes a place of relevance, considering the high prevalence of this pathology in most European countries and in the United States, and is undergoing a rapid increase in developing countries. Actually, only few models of cardiac cache...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/9549036
更新日期:2016-01-01 00:00:00