Abstract:
:The aim of this study was to trigger the expression of genes related to oocytes in putative ovarian stem cells scraped from the ovarian surface epithelium of women with premature ovarian failure and cultured in vitro in the presence of follicular fluid, rich in substances for oocyte growth and maturation. Ovarian surface epithelium was scraped and cell cultures were set up by scrapings in five women with nonfunctional ovaries and with no naturally present mature follicles or oocytes. In the presence of donated follicular fluid putative stem cells grew and developed into primitive oocyte-like cells. A detailed single-cell gene expression profiling was performed to elucidate their genetic status in comparison to human embryonic stem cells, oocytes, and somatic fibroblasts. The ovarian cell cultures depleted/converted reproductive hormones from the culture medium. Estradiol alone or together with other substances may be involved in development of these primitive oocyte-like cells. The majority of primitive oocyte-like cells was mononuclear and expressed several genes related to pluripotency and oocytes, including genes related to meiosis, although they did not express some important oocyte-specific genes. Our work reveals the presence of putative stem cells in the ovarian surface epithelium of women with premature ovarian failure.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Virant-Klun I,Skutella T,Kubista M,Vogler A,Sinkovec J,Meden-Vrtovec Hdoi
10.1155/2013/861460subject
Has Abstractpub_date
2013-01-01 00:00:00pages
861460eissn
2314-6133issn
2314-6141journal_volume
2013pub_type
杂志文章abstract::Retinal vein occlusion (RVO) is a common vascular disease of retina; however, the pathomechanism leading to RVO is not yet clear. In general, increasing age, hypertension, arteriosclerosis, diabetes mellitus, dyslipidemia, cardiovascular disorder, and cerebral stroke are systemic risk factors of RVO. However, RVO ofte...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/4816527
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9689312
更新日期:2020-08-22 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/9209676
更新日期:2019-04-15 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/640397
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/642520
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/361893
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/2721578
更新日期:2019-04-24 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/7097580
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/472346
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/7243909
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/143504
更新日期:2015-01-01 00:00:00
abstract::[This retracts the article DOI: 10.1155/2014/479180.]. ...
journal_title:BioMed research international
pub_type: 撤回出版物
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更新日期:2016-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/576528
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1155/2017/8132676
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2016/7310694
更新日期:2016-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7042124
更新日期:2020-10-05 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2019/6104738
更新日期:2019-01-29 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/3746326
更新日期:2019-03-07 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/238532
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8624745
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/617569
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/430943
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/323046
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/2019846
更新日期:2019-06-19 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1578643
更新日期:2018-08-13 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/497492
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,收录出版
doi:10.1155/2013/438243
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/549737
更新日期:2013-01-01 00:00:00