Synthesis and degradation of glycogen by Schistosoma mansoni worms in vitro.


:The glycogen stores of adult Schistosoma mansoni worms could be labelled by incubation of the worms, after an initial reduction of their glycogen content, in the presence of [6-14C]glucose. Subsequent breakdown of the labelled glycogen by the parasite revealed that glycogen was degraded to lactate and carbon dioxide. The degradation of glycogen, as compared to that of glucose, resulted in slightly different ratios of these two end-products. This indicates that glycogen breakdown did not replace glucose breakdown to the same extent in all cells and that Krebs-cycle activity was not uniformly distributed throughout the cells of this parasite. Both fructose and mannose could replace glucose as an energy source and the rate of glycogen synthesis from either of these two carbohydrates was higher than from glucose. No indications for glyconeogenesis from C3-units were found. Glycogen metabolism of S. mansoni was not influenced by hormones of the mammalian host. It is regulated by the external glucose concentration and by the level of the endogenous glycogen stores. Studies on paired and unpaired worms showed that no interaction between male and female was necessary for the synthesis of glycogen by female worms.






Tielens AG,Celik C,Van den Heuvel JM,Elfring RH,Van den Bergh SG




Has Abstract


1989-02-01 00:00:00








98 ( Pt 1)


  • Schistosoma mansoni from naturally infected Rattus rattus in Guadeloupe: identification, prevalence and enzyme polymorphism.

    abstract::An enzymatic comparison has been made between isolates of Schistosoma mansoni from rats and humans in Guadeloupe and a Burundi isolate of S. rodhaini. Analyses of LDH, MDH, AcP, PGM, GPI, G6PDH and HK by isoelectric focusing provided no evidence for the involvement of S. rodhaini in the recent evolution of the schisto...


    pub_type: 杂志文章


    authors: Rollinson D,Imbert-Establet D,Ross GC

    更新日期:1986-08-01 00:00:00

  • Low-dose oral administration of human interferon alpha can control the development of Theileria parva infection in cattle.

    abstract::Two natural human interferon alpha preparations, (nHuIFN-alpha [Cantell]) and (nHuIFN-alpha [ISI]), were used for the oral treatment of cattle experimentally infected with Theileria parva parva. In the first experiment, 8 Friesian bulls were inoculated with a 1 in 10 dilution of a sporozoite stabilate of T.p. parva (M...


    pub_type: 杂志文章


    authors: Young AS,Maritim AC,Kariuki DP,Stagg DA,Wafula JM,Mutugi JJ,Cummins JM,Richards AB,Burns C

    更新日期:1990-10-01 00:00:00

  • Matrix metalloproteinases mediate the metastatic phenotype of Theileria annulata-transformed cells.

    abstract::Theileria annulata infects and reversibly transforms bovine leucocytes. The parasite-transformed cells are immortalized, metastatic and express a number of metalloproteinases including matrix metalloproteinase 9 which they secrete. All the metalloproteinases observed on substrate gels are inhibited by tissue inhibitor...


    pub_type: 杂志文章


    authors: Adamson RE,Hall FR

    更新日期:1996-11-01 00:00:00

  • Host-parasite interactions and global climate oscillations.

    abstract::It is suspected that host-parasite interactions are influenced by climatic oscillations such as the North Atlantic Oscillation (NAO). However, the effects of climatic oscillations on host-parasite interactions have never been investigated. A long-term (1982-1999) dataset of the host snail Lymnaea stagnalis and tremato...


    pub_type: 杂志文章


    authors: Doi H,Yurlova NI

    更新日期:2011-07-01 00:00:00

  • Sub-cellular fractionation of Trypanosoma brucei. Isolation and characterization of plasma membranes.

    abstract::A procedure is described for the isolation of sub-cellular fractions from bloodstream forms of Trypanosoma brucei. The method leaves intact most of the nuclei, mitochondria and microbodies. All the fractions have been chemically characterized and tested for 10 enzymatic markers. About 5% of total cell protein was isol...


    pub_type: 杂志文章


    authors: Rovis L,Baekkeskov S

    更新日期:1980-06-01 00:00:00

  • Neospora caninum cytoplasmic dynein LC8 light chain 2 (NcDYNLL2) is differentially produced by pathogenically distinct isolates and regulates the host immune response.

    abstract::Neospora caninum is the causative agent of bovine neosporosis. A N. caninum cytoplasmic dynein LC8 light chain (NcDYNLL) protein was characterized in this study. Cytoplasmic dyneins, including DYNLLs, belong to the microtubule minus-end-directed motor proteins and are involved in many cellular processes. Previous micr...


    pub_type: 杂志文章


    authors: Cao L,Fetterer R,Qu G,Zhang X,Tuo W

    更新日期:2018-12-18 00:00:00

  • The relationship of endotoxaemia to peripheral and central nervous system inflammatory responses in Human African Trypanosomiasis.

    abstract::Endotoxaemia has been described in cases of Human African trypanosomiasis (HAT), but it is unclear if this phenomenon influences inflammatory pathology either in the periphery or central nervous system (CNS). We studied endotoxin concentrations in the plasma and cerebrospinal fluid (CSF) of Trypanosoma brucei rhodesie...


    pub_type: 杂志文章


    authors: Maclean L,Aboubaker EA,Kennedy PG,Sternberg JM

    更新日期:2017-04-01 00:00:00

  • What has proteomics taught us about Leishmania development?

    abstract::Leishmania are obligatory intracellular parasitic protozoa that cycle between sand fly mid-gut and phagolysosomes of mammalian macrophages. They have developed genetically programmed changes in gene and protein expression that enable rapid optimization of cell function according to vector and host environments. During...


    pub_type: 杂志文章,评审


    authors: Tsigankov P,Gherardini PF,Helmer-Citterich M,Zilberstein D

    更新日期:2012-08-01 00:00:00

  • Effects of parasites upon non-host predator avoidance behaviour in native and invasive gammarids.

    abstract::The acanthocephalan parasite, Polymorphus minutus, manipulates its intermediate hosts' (gammarids) behaviour, presumably to facilitate its transmission to the definitive hosts. A fundamental question is whether this capability has evolved to target gammarids in general, or specifically sympatric gammarids. We assessed...


    pub_type: 杂志文章


    authors: Farahani S,Palsbøll PJ,Pen I,Komdeur J

    更新日期:2020-11-13 00:00:00

  • Characterization of Meloidogyne javanica surface coat with antibodies and their effect on nematode behaviour.

    abstract::The surface coat of the 2nd-stage juveniles (J2) of plant-parasitic nematodes is considered to be involved in interactions with microorganisms in the soil and rhizosphere, as well as with the host plant. Characterization of surface antigens might be important in the development of new nematode control strategies. In t...


    pub_type: 杂志文章


    authors: Sharon E,Spiegel Y,Salomon R,Curtis RH

    更新日期:2002-08-01 00:00:00

  • RNA-interference methods for gene-knockdown in the sea louse, Lepeophtheirus salmonis: studies on a putative prostaglandin E synthase.

    abstract::Harnessing the full utility of extensive gene sequences recently available for the economically important sea louse, Lepeophtheirus salmonis, requires the adaptation of modern molecular biology approaches to this non-model organism. Using a putative microsomal prostaglandin E synthase type-2 (PGES2) as a candidate gen...


    pub_type: 杂志文章


    authors: Campbell EM,Pert CC,Bowman AS

    更新日期:2009-07-01 00:00:00

  • Immunochemical analysis of surface membrane antigens on erythrocytes infected with non-cloned SICA[+] or cloned SICA[-] Plasmodium knowlesi.

    abstract::The SICA[-] or non-agglutinable phenotype of Plasmodium knowlesi schizont-infected erythrocytes has been defined serologically but not biochemically. Similarly, non-cloned SICA[+] or agglutinable parasites have been shown serologically to express SICA or variant antigen(s) but the number and nature of such antigens ha...


    pub_type: 杂志文章


    authors: Howard RJ,Barnwell JW

    更新日期:1985-10-01 00:00:00

  • Kinetics of classes and sub-classes of total immunoglobulins and specific antibodies to Schistosoma mansoni during murine infection.

    abstract::During the course of Schistosoma mansoni murine infection there is a dramatic increase of some immunoglobulins and S. mansoni-specific antibodies. The most substantial response is initiated after 40 days of infection and results in a prolonged increase of total IgG1, IgM and IgA. The maximum increase is respectively 2...


    pub_type: 杂志文章


    authors: Bout D,Rousseaux R,Carlier Y,Capron A

    更新日期:1980-04-01 00:00:00

  • The attachment of Schistosoma mansoni cercariae to human skin cells.

    abstract::Most of our knowledge about the process of penetration of skin, by cercariae of Schistosoma mansoni, has been gained from studies carried out in vivo with laboratory animals. Human skin is significantly different from that of other animals but there are obvious practical difficulties in directly studying attachment an...


    pub_type: 杂志文章


    authors: Khammo N,Bartlett A,Clothier RH,Whitfield PJ

    更新日期:2002-01-01 00:00:00

  • Integration and expression of murine retrovirus-related sequences in schistosomes.

    abstract::Antibodies against the retrovirus envelope glycoprotein (gp70) of mouse xenotropic retrovirus, BALB virus 2 (Bv2) reacted with the adult worms of Schistosoma japonicum and S. mansoni. This reaction was completely inhibited after adsorption of the antibodies with virions of retrovirus. The reactive schistosome antigen ...


    pub_type: 杂志文章


    authors: Tanaka M,Iwamura Y,Amanuma H,Irie Y,Watanabe M,Watanabe T,Uchiyama Y,Yasuraoka K

    更新日期:1989-08-01 00:00:00

  • Characterization of two cDNAs encoding cysteine proteinases from the soybean cyst nematode Heterodera glycines.

    abstract::Two cDNAs encoding cysteine proteinases were isolated from a cDNA library constructed from feeding females of Heterodera glycines. The library was screened with a cysteine proteinase gene fragment originally amplified from cDNA of H. glycines. Database searches predict that 1 cDNA (hgcp-I) encodes a cathepsin L-like p...


    pub_type: 杂志文章


    authors: Urwin PE,Lilley CJ,McPherson MJ,Atkinson HJ

    更新日期:1997-06-01 00:00:00

  • Detection of circulating trypanosomal antigens by double antibody ELISA using antibodies to procyclic trypanosomes.

    abstract::A double antibody sandwich ELISA technique has been developed for detection of antigens of African trypanosomes present in the sera of infected mammals. The assay uses a high titre, high affinity rabbit antiserum made to purified membranes of procyclic trypanosomes as "capture" reagent and a mixture of three biotin-la...


    pub_type: 杂志文章


    authors: Liu MK,Pearson TW

    更新日期:1987-10-01 00:00:00

  • Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences.

    abstract::Phylogenetic relationships among Trypanosoma rangeli isolates from man, wild mammals and triatomine bugs from widespread geographical origin were inferred by comparison of the small subunit of ribosomal gene sequences. The phylogenetic trees indicated that the subgenus Herpetosoma is polyphyletic and strongly supporte...


    pub_type: 杂志文章


    authors: Da Silva FM,Noyes H,Campaner M,Junqueira AC,Coura JR,Añez N,Shaw JJ,Stevens JR,Teixeira MM

    更新日期:2004-11-01 00:00:00

  • Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study.

    abstract::The recent development of genetic methods allows the delineation of species boundaries, especially in organisms where morphological characters are not reliable to differentiate species. However, few empirical studies have used these tools to delineate species among parasitic metazoans. Here we investigate the species ...


    pub_type: 杂志文章


    authors: Pérez-Ponce DE León G,García-Varela M,Pinacho-Pinacho CD,Sereno-Uribe AL,Poulin R

    更新日期:2016-11-01 00:00:00

  • Glycans with N-acetyllactosamine type 2-like residues covering adult Schistosoma mansoni, and glycomimesis as a putative mechanism of immune evasion.

    abstract::Glycans at the surface of adult Schistosoma mansoni were investigated with gold-labelled lectins. The fragile complex of the glycans with the outer membranes could be preserved for electron microscopy by avoiding extensive pre-fixation with aldehydes and by introducing osmium-ferrocyanide as a membrane fixative. Male ...


    pub_type: 杂志文章


    authors: Schmidt J

    更新日期:1995-09-01 00:00:00

  • Chemotactic behaviour of Strongyloides stercoralis infective larvae on a sodium chloride gradient.

    abstract::Chemotactic responses of Strongyloides stercoralis infective larvae (L3) to sodium chloride (NaCl) were investigated by recording larval tracks on a saline gradient in agarose. On agarose, larvae migrated randomly, whereas when placed at 0.01 m NaCl larvae moved to approximately 1.1 m NaCl where they turned, headed do...


    pub_type: 杂志文章


    authors: Forbes WM,Ashton FT,Boston R,Schad GA

    更新日期:2003-08-01 00:00:00

  • Altered lysophospholipase B responsiveness in lactating mice infected with intestinal nematode parasites.

    abstract::Lactating and nulliparous outbred Swiss (CF-1 strain) mice were infected at 12-16 weeks of age with Nippostrongylus brasiliensis or Trichinella spiralis. Lysophospholipase B levels in the intestinal tissue and faecal pellets were greatly in infected lactating mice in contrast to infected nulliparous mice. Correlated w...


    pub_type: 杂志文章


    authors: Ngwenya BZ

    更新日期:1980-08-01 00:00:00

  • An agent-based model of exposure to human toxocariasis: a multi-country validation.

    abstract::Seroprevalence data illustrate that human exposure to Toxocara is frequent. Environmental contamination with Toxocara spp. eggs is assumed to be the best indicator of human exposure, but increased risk of exposure has also been associated with many other factors. Reported associations are inconsistent, however, and th...


    pub_type: 杂志文章


    authors: Kanobana K,Devleesschauwer B,Polman K,Speybroeck N

    更新日期:2013-07-01 00:00:00

  • Apis mellifera venom induces different cell death pathways in Trypanosoma cruzi.

    abstract::Chagas disease chemotherapy is based on drugs that exhibit toxic effects and have limited efficacy, such as Benznidazole. Therefore, research into new chemotherapeutic agents from natural sources needs to be exploited. Apis mellifera venom consists of many biologically active molecules and has been reported to exhibit...


    pub_type: 杂志文章


    authors: Adade CM,Chagas GS,Souto-Padrón T

    更新日期:2012-09-01 00:00:00

  • Avian malaria, ecological host traits and mosquito abundance in southeastern Amazonia.

    abstract::Avian malaria is a vector transmitted disease caused by Plasmodium and recent studies suggest that variation in its prevalence across avian hosts is correlated with a variety of ecological traits. Here we examine the relationship between prevalence and diversity of Plasmodium lineages in southeastern Amazonia and: (1)...


    pub_type: 杂志文章


    authors: Fecchio A,Ellis VA,Bell JA,Andretti CB,D'Horta FM,Silva AM,Tkach VV,Weckstein JD

    更新日期:2017-07-01 00:00:00

  • Characterization of surface proteins and glycoproteins on red blood cells from mice infected with haemosporidia: Plasmodium berghei infections of BALB/c mice.

    abstract::The surface proteins and glycoproteins of red cells from Plasmodium berghei-infected blood have been radio-isotope labelled and compared with those of normal mouse erythrocytes using the following protein labelling probes: lactoperoxidase-catalysed radio-iodination of tyrosyl residues, periodate oxidation and NaB3H4 r...


    pub_type: 杂志文章


    authors: Howard RJ,Smith PM,Mitchell GF

    更新日期:1980-10-01 00:00:00

  • Functional characterization of X-prolyl aminopeptidase from Toxoplasma gondii.

    abstract::In the present study, a recombinant aminopeptidase P (rTgAPP) from Toxoplasma gondii was expressed in Escherichia coli to evaluate its enzyme parameters. The rTgAPP showed strong activity against a synthetic substrate for aminopeptidase P at pH 8·0 with a K m value of 0·255 µ m and a k cat value of 35·6 s-1. The overa...


    pub_type: 杂志文章


    authors: Yang M,Zheng J,Jia H,Song M

    更新日期:2016-09-01 00:00:00

  • Detection of eight Cryptosporidium genotypes in surface and waste waters in Europe.

    abstract::Cryptosporidia are important protozoan parasites of vertebrates, and a number of species and genotypes, with different host ranges, have been described. In this study a protocol was established for the detection and the genetic characterization of Cryptosporidium spp. isolated from various types of surface waters (riv...


    pub_type: 杂志文章


    authors: Ward PI,Deplazes P,Regli W,Rinder H,Mathis A

    更新日期:2002-04-01 00:00:00

  • Methods in coccidiosis research: separation of oocysts from faeces.

    abstract::Factors which may be important in the large-scale extraction of coccidial oocysts from faeces been investigated with Eimeria tenella. Age of bird, inoculum, feeding status at the time of inoculation, period of collection, feeding status during collection, collection medium, homogenization and sieving, flotation,...


    pub_type: 杂志文章


    authors: Ryley JF,Meade R,Hazelhurst J,Robinson TE

    更新日期:1976-12-01 00:00:00

  • Structure of three high voltage-activated calcium channel alpha1 subunits from Schistosoma mansoni.

    abstract::Voltage-gated calcium (Ca2+) channels contribute to impulse propagation in excitable cells and also regulate intracellular levels of Ca2+. High voltage-activated (HVA) Ca2+ channels are heteromultimeric membrane proteins. The pore-forming, voltage-sensing subunit is the alpha1 subunit. We have cloned 3 HVA Ca2+ channe...


    pub_type: 杂志文章


    authors: Kohn AB,Lea J,Roberts-Misterly JM,Anderson PA,Greenberg RM

    更新日期:2001-11-01 00:00:00