Variation of transient gene expression within single lineages of Trypanosoma cruzi.

Abstract:

:The biodiversity of Trypanosoma cruzi is one of the main factors complicating the understanding of its molecular epidemiology. As an alternative to classical genetic methods, investigators have used DNA-mediated transformation techniques to study this diversity. Recently, transient expression data were shown to correlate with the genetic data. This led investigators to speculate on a potential speciation within clonal populations of Trypanosoma cruzi. To further test the phylogenetic significance of transient expression analysis in Trypanosoma cruzi, multiple plasmids were used to drive the expression of a reporter gene in different parasite populations. In our study, population specific expression of the reporter gene was observed but the variability revealed by these transient expression assays was very large and did not follow the grouping of Trypanosoma cruzi populations in 2 lineages. In this report, we discuss some of the limitations of transient assays on such a diverse parasite.

journal_name

Parasitology

journal_title

Parasitology

authors

Laurent JP,Swindle J

doi

10.1017/s0031182099005053

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

583-9

eissn

0031-1820

issn

1469-8161

journal_volume

119 ( Pt 6)

pub_type

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