Abstract:
:The biodiversity of Trypanosoma cruzi is one of the main factors complicating the understanding of its molecular epidemiology. As an alternative to classical genetic methods, investigators have used DNA-mediated transformation techniques to study this diversity. Recently, transient expression data were shown to correlate with the genetic data. This led investigators to speculate on a potential speciation within clonal populations of Trypanosoma cruzi. To further test the phylogenetic significance of transient expression analysis in Trypanosoma cruzi, multiple plasmids were used to drive the expression of a reporter gene in different parasite populations. In our study, population specific expression of the reporter gene was observed but the variability revealed by these transient expression assays was very large and did not follow the grouping of Trypanosoma cruzi populations in 2 lineages. In this report, we discuss some of the limitations of transient assays on such a diverse parasite.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Laurent JP,Swindle Jdoi
10.1017/s0031182099005053subject
Has Abstractpub_date
1999-12-01 00:00:00pages
583-9eissn
0031-1820issn
1469-8161journal_volume
119 ( Pt 6)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Lactating and nulliparous outbred Swiss (CF-1 strain) mice were infected at 12-16 weeks of age with Nippostrongylus brasiliensis or Trichinella spiralis. Lysophospholipase B levels in the intestinal tissue and faecal pellets were greatly in infected lactating mice in contrast to infected nulliparous mice. Correlated w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000054998
更新日期:1980-08-01 00:00:00
abstract::Most of our knowledge about the process of penetration of skin, by cercariae of Schistosoma mansoni, has been gained from studies carried out in vivo with laboratory animals. Human skin is significantly different from that of other animals but there are obvious practical difficulties in directly studying attachment an...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200100885x
更新日期:2002-01-01 00:00:00
abstract::Toxoplasma gondii has a unique enzyme, a NTPase, which has a wide specificity toward NTP. In the present study, we produced a monoclonal antibody (IgG1, 6C6) against the enzyme which could recognize NTPase isozymes among several strains of T. gondii. Three avirulent strains of T. gondii, ME49, Beverley and Nakayama, w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000007101
更新日期:2001-01-01 00:00:00
abstract::The effect of sainfoin (Onobrychis viciifolia) hay consumption on the pathophysiology and local cellular responses of growing lambs during infection with Trichostrongylus colubriformis was investigated. Thirty-two lambs, 16 weeks of age, were allocated to 1 of 4 treatment groups (n=8) that were offered either grass (G...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008004563
更新日期:2008-08-01 00:00:00
abstract::Balb/c mice infected with the gastrointestinal nematode Heligmosomoides bakeri were fed protein sufficient (PS, 24%) or deficient (PD, 3%) diets to investigate whether diet, infection or dose of larval challenge (0, 100 or 200 larvae) influenced gut pathophysiology and inflammation. Among the PS mice, worms were more ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182007003617
更新日期:2008-01-01 00:00:00
abstract::Differentiation of epimastigotes and production of infective metacyclic forms of Trypanosoma congolense were examined in a culture system which enabled manipulation of the population density of insect forms. Scanning electron microscopy of cultures revealed the attachment sites of epimastigotes in detail, showing them...
journal_title:Parasitology
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Hybridization events between Schistosoma species (Digenea, Platyhelminthes) are reported with increasing frequency, largely due to improved access to molecular tools. Nevertheless, little is known about the distribution and frequency of hybrid schistosomes in nature. Screening for hybrids on a large scale is complicat...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182018000525
更新日期:2018-04-01 00:00:00
abstract::Pinewood nematode, Bursaphelenchus xylophilus, a pine parasitic nematode, poses a serious threat to its host pine forests globally. When dispersal-stage larvae 4 (dauer, DL4) of B. xylophilus enters the new pine, it moults into propagative adult (dauer recovery) and reproduces quickly to kill the host pine. Here, we f...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019001264
更新日期:2020-01-01 00:00:00
abstract::Freshwater snails of the Bulinus forskalii group are one of four Bulinus species complexes responsible for the transmission of schistosomes in Africa and adjacent regions. The species status of these conchologically variable and widely distributed planorbids remains unclear, and parasite compatibility varies considera...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001008381
更新日期:2001-01-01 00:00:00
abstract::Parasites can have a wide range of pathophysiological effects on the host. This review describes those associated with some parasites of major importance in man and animals. Haemoprotozoan diseases such as trypanosomiasis and malaria are primarily associated with anaemia. Such anaemias have a complex aetiology involvi...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000085814
更新日期:1987-01-01 00:00:00
abstract::Sequence comparisons have recently shown that the Schistosoma mansoni protein Sm32 is similar to asparaginyl endoproteinases, a novel family of cysteine proteinases, of which the legumains from legumes are the best characterized. By synthesizing and employing fluorogenic peptide substrates for the specific detection o...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000077052
更新日期:1995-12-01 00:00:00
abstract::Feulgen-pararosaniline (SO2) staining was performed on stages in the life-cycle of Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei. The fluorescence emission of the stained DNA in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000054305
更新日期:1984-02-01 00:00:00
abstract::Immunization against the protozoan Theileria parva by infection and treatment has proved to be very efficient for the control of East Coast fever, an acute and often-fatal lymphoproliferative tick-borne disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. parva sporozoites used in ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182003004323
更新日期:2004-02-01 00:00:00
abstract::Polysporoplasma sparis infection was studied in gilthead sea bream from different mariculture systems of the Spanish coasts. Culture conditions influenced the infection dynamics, as the parasite appeared only in semi-intensive cultures and was not found in intensive closed systems nor in open ones. No clear seasonal p...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098003758
更新日期:1999-03-01 00:00:00
abstract::In this study, we describe the molecular analysis of zinc-metalloproteases from the abomasal nematode Ostertagia ostertagi which were exclusively recognized by local antibodies of immune cattle. Full-length or partial coding sequences of 4 different zinc-metalloprotease cDNAs of Ostertagia (met-1, -2, -3 and -4) were ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006274
更新日期:2005-01-01 00:00:00
abstract::The parasitic roundworms Strongyloides stercoralis (in man) and Strongyloides ratti (in rats) employ environmentally controlled XX/XO sex determination with a pair of X chromosomes and two pairs of autosomes. Strongyloides papillosus (in sheep) has only two pairs of chromosomes, one of which combines the genetic mater...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013001315
更新日期:2013-12-01 00:00:00
abstract::The SICA[-] or non-agglutinable phenotype of Plasmodium knowlesi schizont-infected erythrocytes has been defined serologically but not biochemically. Similarly, non-cloned SICA[+] or agglutinable parasites have been shown serologically to express SICA or variant antigen(s) but the number and nature of such antigens ha...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000057346
更新日期:1985-10-01 00:00:00
abstract::Bat flies are obligate ectoparasites of bats and it has been hypothesized that they may be involved in the transmission of Bartonella species between bats. A survey was conducted to identify whether Cyclopodia greefi greefi (Diptera: Nycteribiidae) collected from Ghana and 2 islands in the Gulf of Guinea harbour Barto...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011002113
更新日期:2012-03-01 00:00:00
abstract::Intraerythrocytic malaria parasites ingest the cytosol of their host cell and digest it inside their acid food vacuoles. Acidified (pH 4-5.5, 37 degrees C) human red blood cell lysates were used to simulate this process, measuring the denaturation of haemoglobin (Hb) and the release of iron, in the absence or presence...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000075910
更新日期:1994-05-01 00:00:00
abstract::Plant cysteine proteinases (CPs) from Carica papaya kill parasitic and free-living nematodes in vitro by hydrolysis of the worm cuticle, a mechanism that is different to all commercially available synthetic anthelmintics. We have developed a cheap and effective, rapid-throughput Caenorhabditis elegans-based assay for ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002353
更新日期:2017-04-01 00:00:00
abstract::In an experiment to study the infectivity, growth and virulence of Schistocephalus solidus in their first intermediate host, copepods of the species Macrocyclops albidus were kept singly and exposed to up to 9 coracidia. Eleven or 14 days post-infection (p.i.) the presence and growth of the cestode larvae relative to ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182097001406
更新日期:1997-09-01 00:00:00
abstract::The global seroprevalence of latent toxoplasmosis is estimated to be higher than 30%. The presence of slowly dividing parasites in tissue cysts located mainly in immunoprivileged organs was long considered asymptomatic. Recently, many studies have shown that latent Toxoplasma infections could have serious impacts on h...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001785
更新日期:2016-12-01 00:00:00
abstract::The capacity of Acanthocheilonema viteae to metabolize fructose was investigated in vitro. In common with other filarial species A. viteae oxidized fructose to lactate but its rate of consumption was only 40% of the glucose-containing control value. Fructose was not incorporated into glycogen. Release of 14CO2 from [U...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060649
更新日期:1990-12-01 00:00:00
abstract::Two natural human interferon alpha preparations, (nHuIFN-alpha [Cantell]) and (nHuIFN-alpha [ISI]), were used for the oral treatment of cattle experimentally infected with Theileria parva parva. In the first experiment, 8 Friesian bulls were inoculated with a 1 in 10 dilution of a sporozoite stabilate of T.p. parva (M...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000063241
更新日期:1990-10-01 00:00:00
abstract::The individual and combined effects of cadmium (Cd) and 3,3',4,4',5-pentachlorobiphenyl (PCB 126) on the antibody response of fish against metazoan parasites were tested. Eels experimentally infected with the swim bladder nematode Anguillicola crassus were exposed to sublethal concentrations of Cd and PCB 126. Cd was ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200300475x
更新日期:2004-04-01 00:00:00
abstract::Humans and other hosts acquire Toxoplasma gondii infection by ingesting tissue cysts in undercooked meat, or by food or drink contaminated with oocysts. Currently, there is no vaccine to prevent clinical disease due this parasite in humans, although, various T. gondii vaccine candidates are being developed. Mice are g...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001673
更新日期:2012-01-01 00:00:00
abstract::Biochemical characterization of 137 Leishmania braziliensis isolates from South and Central America, and from selected endemic foci in Bolivia, Brazil and Colombia, performed by isoenzymatic electrophoresis using 10 enzymatic systems, showed a high enzymatic polymorphism (44 zymodemes obtained) based on the variation ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182097001376
更新日期:1997-10-01 00:00:00
abstract::In a recent study by our group on the prevalence of intestinal parasitic infections and on the knowledge, attitudes and practices of local residents of Campos dos Goytacazes, Rio de Janeiro, Brazil, we observed that about 50% of the inhabitants were parasitized and had some knowledge of intestinal parasitic infections...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011002253
更新日期:2012-05-01 00:00:00
abstract::This cross-sectional study aimed to determine the prevalence and risk factors of S. stercoralis infection among 1142 Orang Asli primary schoolchildren in six different states of Peninsular Malaysia. Fecal samples were examined using direct smear, formalin-ether sedimentation (FES), agar plate culture (APC) and PCR tec...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019000945
更新日期:2019-10-01 00:00:00
abstract::Sera from goats and cattle that were infected with two Trypanosoma vivax clones (ILDat 1.2 and ILDat 2.1) derived from different stocks were analysed for antibody activity against the variable surface glycoproteins (VSGs) of the infecting clones by enzyme-linked immune assays (ELISA) and immune lysis. To obtain purifi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060157
更新日期:1990-02-01 00:00:00