Recombinant dense granular protein (GRA5) for detection of human toxoplasmosis by Western blot.

Abstract:

:Toxoplasma gondii infects all warm-blooded animals, including humans, causing serious public health problems and great economic loss for the food industry. Commonly used serological tests require costly and hazardous preparation of whole Toxoplasma lysate antigens from tachyzoites. Here, we have evaluated an alternative method for antigen production, which involved a prokaryotic expression system. Specifically, we expressed T. gondii dense granular protein-5 (GRA5) in Escherichia coli and isolated it by affinity purification. The serodiagnostic potential of the purified recombinant GRA5 (rGRA5) was tested through Western blot analysis against 212 human patient serum samples. We found that rGRA5 protein was 100% specific for analysis of toxoplasmosis-negative human sera. Also, rGRA5 was able to detect acute and chronic T. gondii infections (sensitivities of 46.8% and 61.2%, resp.).

journal_name

Biomed Res Int

authors

Ching XT,Lau YL,Fong MY,Nissapatorn V,Andiappan H

doi

10.1155/2014/690529

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

690529

eissn

2314-6133

issn

2314-6141

journal_volume

2014

pub_type

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