Abstract:
:Toxoplasma gondii infects all warm-blooded animals, including humans, causing serious public health problems and great economic loss for the food industry. Commonly used serological tests require costly and hazardous preparation of whole Toxoplasma lysate antigens from tachyzoites. Here, we have evaluated an alternative method for antigen production, which involved a prokaryotic expression system. Specifically, we expressed T. gondii dense granular protein-5 (GRA5) in Escherichia coli and isolated it by affinity purification. The serodiagnostic potential of the purified recombinant GRA5 (rGRA5) was tested through Western blot analysis against 212 human patient serum samples. We found that rGRA5 protein was 100% specific for analysis of toxoplasmosis-negative human sera. Also, rGRA5 was able to detect acute and chronic T. gondii infections (sensitivities of 46.8% and 61.2%, resp.).
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Ching XT,Lau YL,Fong MY,Nissapatorn V,Andiappan Hdoi
10.1155/2014/690529subject
Has Abstractpub_date
2014-01-01 00:00:00pages
690529eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract::The aims of this study were to evaluate the contribution of chromosomal microarray analysis (CMA) in the prenatal diagnosis of fetuses with central nervous system (CNS) anomalies but normal chromosomal karyotype. A total of 46 fetuses with CNS anomalies with or without other ultrasound anomalies but normal karyotypes ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/426379
更新日期:2015-01-01 00:00:00
abstract:Aims:We aimed to summarize available lines of evidence about intraoperative and postoperative donor outcomes following robotic-assisted laparoscopic donor nephrectomy (RALDN) as well as outcomes of graft and recipients. Methods:A systematic review of PubMed/Medline, ISI Web of Knowledge, and Scopus databases was perfo...
journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2019/1729138
更新日期:2019-04-22 00:00:00
abstract:Objective:The aim of this study was to investigate the respective correlation between the height (H) of a posterior malleolar fracture (PMF) and the involved area (S) of an articular surface and the presence of "die-punch." Methods:Patients with closed posterior malleolar fractures admitted to our hospital from Januar...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/2903537
更新日期:2020-07-29 00:00:00
abstract::Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the mat...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/364930
更新日期:2014-01-01 00:00:00
abstract::Protein phosphorylation is one of the most widespread regulatory mechanisms in eukaryotes. Over the past decade, phosphorylation site prediction has emerged as an important problem in the field of bioinformatics. Here, we report a new method, termed Random Forest-based Phosphosite predictor 2.0 (RF-Phos 2.0), to predi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3281590
更新日期:2016-01-01 00:00:00
abstract::Caudal epidural block is a commonly used technique for surgical anesthesia in children and chronic pain management in adults. It is performed by inserting a needle through the sacral hiatus to gain entrance into the sacral epidural space. Using conventional blind technique, the failure rate of caudal epidural block in...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/9217145
更新日期:2017-01-01 00:00:00
abstract:Background:Overuse of antibiotics is a major driver for rapid spread of antimicrobial resistance worldwide, particularly common in China. The close linkage between hospital revenue and sales of drugs has become the key incentive for overprescription of antibiotics. Since 2009, the Chinese government implemented a serie...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9262170
更新日期:2020-10-22 00:00:00
abstract::One of the most important tasks of a living organism is to maintain its genetic integrity with respect to stress. Endoplasmic reticulum (ER) has a crucial role in sensing cellular homeostasis by controlling metabolism, proteostasis, and several signaling processes. ER stressors can induce autophagy-dependent survival;...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/319589
更新日期:2015-01-01 00:00:00
abstract::Vitamin D and its cognate intracellular nuclear receptor, namely, vitamin D receptor (VDR), are involved in the regulation of a variety of body metabolic processes, immune function, and oncogenesis. A large number of studies demonstrated the association of low vitamin D levels and variations in five common single nucl...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7562958
更新日期:2020-06-15 00:00:00
abstract:Objective:Neuroimaging studies revealed the functional reorganization or the structural changes during stroke recovery. However, previous studies did not combine the functional and structural information and the results might be affected by heterogeneous lesion. This study aimed to investigate functional activation-inf...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/4345205
更新日期:2017-01-01 00:00:00
abstract::We aimed to assess the prevalence of hospitalization for endometriosis in the general population in France and in each French region and to describe temporal trends, rehospitalization rates, and prevalence of the different types of endometriosis. The analyses were carried out on French hospital discharge data and cove...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3260952
更新日期:2016-01-01 00:00:00
abstract::Uraemia and long-term peritoneal dialysis (PD) can lead to fibrotic thickening of the peritoneal membrane, which may limit its dialytic function. Peritoneal fibrosis is associated with the appearance of myofibroblasts and expansion of extracellular matrix. The extent of contribution of resident peritoneal fibroblasts ...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/134708
更新日期:2015-01-01 00:00:00
abstract::Trypanosoma cruzi causes a cardiac infection characterized by an inflammatory imbalance that could become the inciting factor of the illness. To this end, we evaluated the role of carvedilol, a beta-blocker with potential immunomodulatory properties, on the immune response in C57BL/6 mice infected with VL-10 strain of...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9205062
更新日期:2017-01-01 00:00:00
abstract::Tanshinone IIA (Tan IIA), an active phytochemical in the dried root of Salvia miltiorrhiza Bunge, has shown an antiproliferative activity on various human cancer cell lines including nasopharyngeal carcinoma cells. However, the effects of Tan IIA on human oral cancer cells are still unknown. This study aimed to invest...
journal_title:BioMed research international
pub_type: 杂志文章,收录出版
doi:10.1155/2014/540516
更新日期:2014-01-01 00:00:00
abstract::Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune syste...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/143504
更新日期:2015-01-01 00:00:00
abstract::Background. The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms. Methods. HUVECs were cultured and stimulated with different concentrations of H2O2. Flow cytometric analysis was u...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/6895730
更新日期:2017-01-01 00:00:00
abstract::(i) Purpose. The fluid challenge (FC) is a well-established test of preload reserve. Only limited data exist in regard to the FC efficacy based on infusion time. Slow administration may be associated with lack of effect based on fluid redistribution and external conditions changes. On the contrary, fast administration...
journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2018/8925345
更新日期:2018-11-01 00:00:00
abstract::It is increasingly affirmed that most of the long-term consequences of TBI are due to molecular and cellular changes occurring during the acute phase of the injury and which may, afterwards, persist or progress. Understanding how to prevent secondary damage and improve outcome in trauma patients, has been always a tar...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/736104
更新日期:2015-01-01 00:00:00
abstract::Purpose. We examined the role of miR-20b in development of thymoma-associated myasthenia gravis, especially in T cell proliferation and activation. Materials and Methods. Using qRT-PCR, we assessed expression levels of miR-20b and its target genes in cultured cells and patient samples and examined the proliferation of...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/9595718
更新日期:2016-01-01 00:00:00
abstract::Owing to its hydrophilicity, negative charge, small size, and labile degradation by endogenous nucleases, small interfering RNA (siRNA) delivery must be achieved by a carrier system. In this study, cationic copolymers composed of low-molecular-weight polyethylenimine and polythiophenes were synthesized and evaluated a...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/406389
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Sarcopenia is the presence of low muscle mass and low muscle function. The aim of this study was to establish cutoffs for low muscle mass using three published methods and to compare the prevalence of sarcopenia in older Australians. METHODS:Gender specific cutoffs levels were identified for low muscle mass...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/361790
更新日期:2014-01-01 00:00:00
abstract::Long-term application and extensive use of synthetic insecticides have resulted in accumulating their residues in food, milk, water, and soil and cause adverse health effects to human and ecosystems. Therefore, application of natural insecticides in agriculture and public health sectors has been increased as alternati...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/4308054
更新日期:2018-10-16 00:00:00
abstract::Coronary CT angiography (CCTA) has become an important noninvasive imaging modality in the diagnosis of coronary artery disease (CAD). CCTA enables accurate evaluation of coronary artery stenosis. However, CCTA provides limited information on the physiological significance of stenotic lesions. A noninvasive "one-stop-...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/435737
更新日期:2015-01-01 00:00:00
abstract::Multiple sclerosis (MS) is an inflammatory demyelinating disease of the human central nervous system (CNS). Neurotropic demyelinating strain of MHV (MHV-A59 or its isogenic recombinant strain RSA59) induces MS-like disease in mice mediated by microglia, along with a small population of T cells. The mechanism of demyel...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/589048
更新日期:2013-01-01 00:00:00
abstract::Libidibia ferrea (LF) is a medicinal plant that holds many pharmacological properties. We evaluated the antinociceptive effect in the LF aqueous seed extract and Lipidic Portion of Libidibia ferrea (LPLF), partially elucidating their mechanisms. Histochemical tests and Gas chromatography of the LPLF were performed to ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/508725
更新日期:2014-01-01 00:00:00
abstract::We have developed a new "drug" and approach that appear to be effective in reducing arterial age. This "drug" represents a low, subtherapeutic dose of statin and sartan and particularly their low-dose combination. The improvement of arterial wall characteristics, also reflecting in a decrease of arterial age, was achi...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/235709
更新日期:2015-01-01 00:00:00
abstract:Objective:Comparison of outcomes between RIRS with vacuum-assisted ureteral access sheath (V-UAS) and MPCNL in the treatment of renal stone. Materials and Methods:28 patients with 2-4 cm renal stone were treated using RIRS with 14/16 F V-UAS. The outcomes were compared to those who underwent MPCNL with 16 F Amplatz sh...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8052013
更新日期:2020-05-14 00:00:00
abstract::Colorectal cancer (CRC) is a common malignancy with high morbidity and mortality worldwide. To date, chemotherapy plays an important role in the treatment of CRC patients. Multidrug resistance (MDR) is one of the major hurdles in chemotherapy for CRC, and the underlying mechanisms need to be explored. Studies have dem...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/9390878
更新日期:2020-03-18 00:00:00
abstract::Transcatheter treatment of children with congenital heart disease such as coarctation of the aorta and pulmonary artery stenosis currently involves the use of metal stents. While these provide good short term results, there are long term complications with their use. Children outgrow metal stents, obligating them to f...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/137985
更新日期:2013-01-01 00:00:00
abstract::GABAergic inhibition and particularly perisomatic inhibition play a crucial role in controlling the firing properties of large principal cell populations. Furthermore, GABAergic network is a key element in the therapy attempting to reduce epileptic activity. Here, we present a review showing the synaptic changes of pe...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/7154295
更新日期:2017-01-01 00:00:00