Abstract:
:Owing to its hydrophilicity, negative charge, small size, and labile degradation by endogenous nucleases, small interfering RNA (siRNA) delivery must be achieved by a carrier system. In this study, cationic copolymers composed of low-molecular-weight polyethylenimine and polythiophenes were synthesized and evaluated as novel self-tracking siRNA delivery vectors. The concept underlying the design of these copolymers is that hydrophobicity and rigidity of polythiophenes should enhance the transport of siRNA across the cell membrane and endosomal membrane. A gel retardation assay showed that the nanosized complexes formed between the copolymers and siRNA were stable even at a molar ratio of 1 : 2. The high cellular uptake (>80%) and localization of the copolymer vectors inside the cells were easily analyzed by tracking the fluorescence of polythiophene using fluorescent microscopy and cytometry. An in vitro luciferase knockdown (KD) assay in A549-luc cells demonstrated that the siRNA complexes with more hydrophobic copolymers achieved a higher KD efficiency of 52.8% without notable cytotoxicity, indicating protein-specific KD activity rather than solely the cytotoxicity of the materials. Our polythiophene copolymers should serve as novel, efficient, low cell toxicity, and label-free siRNA delivery systems.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
He P,Hagiwara K,Chong H,Yu HH,Ito Ydoi
10.1155/2015/406389subject
Has Abstractpub_date
2015-01-01 00:00:00pages
406389eissn
2314-6133issn
2314-6141journal_volume
2015pub_type
杂志文章abstract:PURPOSE OF THE REPORT:There is a paucity of data on correlation of various imaging modalities with clinical findings in craniosynostosis. Moreover, no study has specifically reported the role of (99m)Tc-ECD SPECT in a large number of subjects with craniosynostosis. MATERIALS AND METHODS:We prospectively analyzed a coh...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/172646
更新日期:2014-01-01 00:00:00
abstract::The anaerobic skin commensal Propionibacterium acnes is an underestimated cause of human infections and clinical conditions. Previous studies have suggested a role for the bacterium in lumbar disc herniation and infection. To further investigate this, five biopsy samples were surgically excised from each of 64 patient...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/530382
更新日期:2013-01-01 00:00:00
abstract::The occurrence of nonalcoholic fatty liver disease (NAFLD) is associated with major abnormalities of hepatic lipid metabolism. We propose that lipid abnormalities directly or indirectly contribute to NAFLD, especially fatty acid accumulation, arachidonic acid metabolic disturbance, and ceramide overload. The effects o...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/4020249
更新日期:2020-07-18 00:00:00
abstract:BACKGROUND:We aimed to identify certain genes related to response to infliximab (IFX) and biomarkers to predict the IFX effect for Japanese Crohn's disease (CD) patients by performing an association study of single nucleotide polymorphisms (SNPs) in candidate genes in the interleukin- (IL-) 17 signaling pathway with re...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/416838
更新日期:2015-01-01 00:00:00
abstract::Blocking glioma cell invasion has been challenging due to cancer cells that can swiftly switch their migration mode, and agents that can block more than one migration mode are sought after. We found that small molecule 2-(1H-indole-3-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE), an endogenous aryl hydrocarb...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/2616930
更新日期:2020-10-03 00:00:00
abstract::Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP) and pyrethroids (PY) are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR) are also employed in the control of larvae. We quantified resistance to temephos (OP), deltamethr...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/8603263
更新日期:2016-01-01 00:00:00
abstract::Polyetheretherketone (PEEK), which has biomechanical performance similar to that of human cancellous bone, is used widely as a spinal implant material. However, its bioinertness and hydrophobic surface properties result in poor osseointegration. This study applies a novel modification method, arc ion plating (AIP), th...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/328943
更新日期:2015-01-01 00:00:00
abstract::Background. For development of individualized treatment on a routine basis, transfer of patients' tumor tissue in a xenograft model (i.e., generation of patient-derived xenografts (PDX)) is desirable for molecular, biochemical, or functional analyses. Drawbacks are dissatisfactory tumor take rates, the necessity of fa...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/1715053
更新日期:2016-01-01 00:00:00
abstract::Since 2001 the Istituto Superiore di Sanità established a quality assurance programme for molecular genetic testing that covers four pathologies: Cystic Fibrosis (CF), Beta Thalassemia (BT), Fragile X Syndrome (FX), and Familial Adenomatous Polyposis Coli (APC). Since 2009 this activity is an institutional activity an...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/739010
更新日期:2013-01-01 00:00:00
abstract::The effect of Cordyceps militaris (CM) grown on germinated soybeans (GSC) in the inflammatory bowel disease (IBD) model was studied. To demonstrate the preventive effect of GSC extract in a dextran-sodium-sulfate- (DSS-) induced acute colitis mouse model, GSC was administered 2 days before DSS coadministration. GSC si...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/102918
更新日期:2013-01-01 00:00:00
abstract:Objective:To evaluate the performance of contrast-enhanced mammography (CEM) compared to magnetic resonance imaging (MRI) for estimating residual tumor size after neoadjuvant chemotherapy (NAC) in women with newly diagnosed breast cancer. Methods:The institutional review board approved this study. This prospective stu...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8531916
更新日期:2018-11-08 00:00:00
abstract:Objectives:The aim of this study was to evaluate the influence of ferule and the post type on the fracture strength and stress distribution in premolars. Materials and Methods:Forty human mandibular premolars were decoronated, allocated in four groups, and restored as follows: CPC-NF: cast post-and-core and absence of...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/2196519
更新日期:2019-01-03 00:00:00
abstract::Presynaptic and postsynaptic neurotoxins are proteins which act at the presynaptic and postsynaptic membrane. Correctly predicting presynaptic and postsynaptic neurotoxins will provide important clues for drug-target discovery and drug design. In this study, we developed a theoretical method to discriminate presynapti...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/3267325
更新日期:2017-01-01 00:00:00
abstract:Objective:Cognitive impairment is considered to be an important complication of spinal cord injury (SCI), but its underlying mechanism remains unclear. The purpose of this study is to explore whether serum CCL21 can be used as a potential biomarker of cognitive impairment in SCI. Methods:In Neck-Shoulder and Lumbocrur...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6692802
更新日期:2020-12-09 00:00:00
abstract::Elevated levels of resistin and epidermal growth factor receptor (EGFR) facilitate the development of breast cancer, although there are no reports of any correlation between these proteins. This study analyzed 392 human breast cancer tissue specimens and 42 samples of adjacent normal tissue. Rates of positive and stro...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8835398
更新日期:2020-11-28 00:00:00
abstract::This study aims to investigate the expression of macrophage inflammatory protein-3 alpha (MIP-3α) and cystatin A in nasopharyngeal carcinoma (NPC) and their association with clinical characteristics and prognosis. Primary tumor specimens from 114 NPC patients and associated clinical follow-up data were collected, and ...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2015/617143
更新日期:2015-01-01 00:00:00
abstract::Hepatitis B virus (HBV) is an important public health problem that poses a serious threat to human health. HBV reactivation generally occurs in overt or occult HBV infection patients who suffered DDAs, chemotherapy, or immunosuppressive therapy, especially when some solid tumors and leukemia patients are using hormone...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/2931402
更新日期:2018-12-26 00:00:00
abstract::Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally controlled fabrication ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/6956794
更新日期:2017-01-01 00:00:00
abstract::Breast cancer is the leading cause of death in women worldwide. The search for novel compounds with antitumor activity, with less adverse effects and higher efficacy, and the development of methods to evaluate their toxicity is an area of intense research. In this study we implemented the preparation and culture of br...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/618021
更新日期:2015-01-01 00:00:00
abstract::Protein phosphorylation is catalyzed by kinases which regulate many aspects that control death, movement, and cell growth. Identification of the phosphorylation site-specific kinase-substrate relationships (ssKSRs) is important for understanding cellular dynamics and provides a fundamental basis for further disease-re...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/1826496
更新日期:2017-01-01 00:00:00
abstract::In the present study, we determined whether there is a delay in the eruption of permanent teeth (PT) among Filipino adolescents with stunting or thinness. Height, weight, and number of PT were recorded in 1554 Filipino 10- to 13-year-olds (711 boys; 843 girls). z-scores for height (HAZ) and body mass index (BMI) were ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/205950
更新日期:2013-01-01 00:00:00
abstract:Background:The purpose of this study was to evaluate the association between the non-high-density lipoprotein cholesterol (non-HDL-C) to high-density lipoprotein cholesterol (HDL-C) ratio and the risk of coronary artery disease (CAD). We also explored the potential role of non-HDL-C/HDL-C in the prognosis of CAD. Meth...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7146028
更新日期:2020-03-07 00:00:00
abstract::Several intestinal parasites of baboons are of zoonotic importance, especially where there is considerable interaction between the baboons and humans. The identification of gastrointestinal parasite infections of baboons (Papio anubis) was undertaken at the Shai Hills Reserve Resort, Ghana. A total of 51 faecal sample...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1083251
更新日期:2020-03-11 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) in TNFSF4 and ANKRD55 genes have been shown to be associated with several autoimmune diseases, although whether these genes are susceptibility genes for dermatomyositis/polymyositis (DM/PM) has, to date, not been reported. This study aimed to investigate the potential association...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2905987
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:This study aimed to investigate the shear displacement between the body and backrest/seat, range of motion (ROM), and force acting on the lower limb joints during sit-stand-sit transitions by operating an electric-powered standing wheelchair. METHODS AND MATERIALS:The amounts of sliding along the backrest and ...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/236486
更新日期:2014-01-01 00:00:00
abstract::Cardiovascular disease (CVD) is the most common cause of death in the world. Recent studies have shown an association between adrenal insufficiency (AI) and increased cardiovascular risk (CVR). Patients with AI receive glucocorticoid (GC) replacement therapy which can lead to varying levels of blood cortisol. It was s...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/3691913
更新日期:2017-01-01 00:00:00
abstract::Family planning coverage has improved in Ethiopia in the last decade, though fertility is still about 5.8 in the rural setup. In this paper, the major structural determinants of family planning service were analyzed using a multilevel model from 8906 individual women observation in the 2011 EDHS data. The results show...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/495745
更新日期:2015-01-01 00:00:00
abstract::This paper represents data of long-term open prospective study. 312 male clean-up workers, who participated in elimination of the Chernobyl disaster consequences in 1986-87, were observed and examined in Moscow Research Institute of Psychiatry. The average age of patients was 57,0 ± 6,8 years. All patients were diagno...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/948473
更新日期:2015-01-01 00:00:00
abstract::Recent studies suggest that nucleotide-binding domain leucine-rich repeat protein 1 (NLRP1) is a pivotal factor in the inflammatory process. However, the role of NLRP1 in breast cancer pathogenesis remains unclear. The aim of this study was to examine the expression and function of NLRP1 in breast cancer. We found tha...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/4938473
更新日期:2017-01-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder caused by selective loss of dopaminergic neurons in the substantia nigra pars compacta which leads to dysfunction of cerebral pathways critical for the control of movements. The diagnosis of PD is based on motor symptoms, such as bradykinesia, akinesia, muscular...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/519816
更新日期:2014-01-01 00:00:00