Abstract:
:Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Milašinović N,Knežević-Jugović Z,Milosavljević N,Lučić Škorić M,Filipović J,Kalagasidis Krušić Mdoi
10.1155/2014/364930subject
Has Abstractpub_date
2014-01-01 00:00:00pages
364930eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
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pub_type: 杂志文章,meta分析,评审
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