Abstract:
:Second-harmonic-generation (SHG) microscopy is a powerful tool for in vivo visualisation of collagen fibres in human skin because of its specific collagen selectivity without the need for staining, non-invasiveness and high-resolution three-dimensional imaging. Although texture analysis of SHG images is a promising method for the quantitative analysis of well-orientated collagen fibre structure in the tendon and cornea, there are few attempts to assess cutaneous ageing. In this study, we applied two texture analysis techniques, namely autocorrelation (2D-AC) analysis and two-dimensional Fourier transform (2D-FT), to evaluate the age-dependent changes in reticular dermal collagen fibres in in vivo human cheek skin. Age-dependent changes in the reticular dermal collagen fibres of female subjects in their 20s, 40s and 60s clearly appeared in these texture analyses. Furthermore, the parameter from 2D-AC analysis showed a significantly higher correlation with skin elasticity measured by a Cutometer® . These results clearly indicate that 2D-AC analysis of SHG images is highly promising for the quantitative evaluation of age-dependent change in facial collagen fibres as well as skin elasticity. An appropriate texture analysis will help to provide quantitative insight into collagen fibre structure and will be useful for the diagnosis of pathological conditions as well as cutaneous ageing in skin.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Ogura Y,Tanaka Y,Hase E,Yamashita T,Yasui Tdoi
10.1111/exd.13560subject
Has Abstractpub_date
2019-08-01 00:00:00pages
899-905issue
8eissn
0906-6705issn
1600-0625journal_volume
28pub_type
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