Abstract:
:Topical photodynamic therapy with 5-aminolevulinic acid (5-ALA) is a newly established treatment modality for epithelial skin cancer. Skin distribution of different 20% 5-ALA formulations was investigated by detection of protoporphyrin IX (PpIX) using an ex vivo porcine skin model. Fluorescence of PpIX was first detectable at 2 h after application of a 5-ALA/HCl-alcohol solution, followed by a 5-ALA gel +/- 40% DMSO > 5-ALA lipophilic ointment > 5-ALA hydrophilic ointment at 4 h after application. Intensity of PpIX was 10-fold higher after 5-ALA/HCl-alcohol application in contrast to 5-ALA hydrophilic ointment. Maxima of PpIX fluorescence were measured between 18 and 24 h after application. PpIX accumulation induced by 5-ALA within the lower parts of the epidermis was increased in order of 5-ALA hydrophilic ointment < 5-ALA lipophilic ointment < 5-ALA gel w/o DMSO < 5-ALA gel with DMSO < 5-ALA/HCl-alcohol. Illumination of skin incubated with different 5-ALA formulations led to a marked increase of apoptotic cells in the epidermis depending on the penetration depth of the 5-ALA formulations. A formulation containing short chain alcohols have been observed to increase the permeation and distribution of 5-ALA. PpIX fluorescence was detected earlier yielding to photodynamic effective amounts of PpIX in the epidermis as compared with all other 5-ALA formulations used. These data emphasizes the potential of investigating properties of new formulations using such a full thickness porcine skin model.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Maisch T,Worlicek C,Babilas P,Landthaler M,Szeimies RMdoi
10.1111/j.1600-0625.2008.00705.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
813-20issue
10eissn
0906-6705issn
1600-0625pii
EXD705journal_volume
17pub_type
杂志文章abstract::CD44 is a family of cell-surface receptors on human lymphocytes that act as co-stimulatory molecules leading to the induction of effector functions in T cells. We have analyzed primary cutaneous malignant melanomas with clinical and histologic signs of tumor regression using immunohistochemistry and observed the predo...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2003.00044.x
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abstract::This study was stimulated by the clinical observation of a rapid response of a chilblain lupus patient to treatment with JAK1/2-kinase inhibitor ruxolitinib. We investigated the in vivo expression of phospho-JAK2 in CLE skin samples as well as the immunomodulatory in vitro effect of ruxolitinib in cultured immortalize...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/exd.13253
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1993.tb00034.x
更新日期:1993-10-01 00:00:00
abstract::As global transcriptome analyses with a growing demand on layer-specific applications are widely used in cutaneous biology, we investigated the effect of established and optimized dermo-epidermal separation methods on the quality of RNA. We compared enzymatic separation with dispase, chemical separation with 1 M sodiu...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2006.00534.x
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abstract::Dermal mast cells protect the skin from inflammatory effects of ultraviolet (UV) radiation and are required for UV-induced immune suppression. We sought to determine a potential mechanistic role of mast cells in reducing the sensitivity to UV radiation (i.e. phototolerance induction) through photohardening. We adminis...
journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1600-0625.2008.00822.x
更新日期:2009-06-01 00:00:00
abstract::Chromatin-IgG complexes appear as electron dense structures (EDS) in glomerular basement membranes in lupus nephritis. Here, we present results of comparative analyses of the composition of EDS in murine lupus dermatitis and nephritis. One focus was to perform an analytical approach to understand why such complex stru...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2010.01064.x
更新日期:2010-08-01 00:00:00
abstract::Nitric oxide (NO) is a potent intercellular mediator of melanogenesis, whereas metallothionein (MT) is an inducible intracellular antioxidant that has been reported to scavenge NO. We investigated the existence and induction of MT in melanocytes, and its inhibitory effect on NO-induced melanogenesis. The expression of...
journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 信件
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12400
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journal_title:Experimental dermatology
pub_type: 评论,杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.12457
更新日期:2014-09-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 评论,杂志文章
doi:10.1111/exd.12194
更新日期:2013-08-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2007.00608.x
更新日期:2007-11-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2009.01037.x
更新日期:2010-08-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.1992.tb00001.x
更新日期:1992-10-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1996.tb00091.x
更新日期:1996-02-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2006.00540.x
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.0906-6705.2004.00201.x
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abstract::This review focuses on the photoprotection conferred by lycopene, one of the most potent anti-oxidants. Lycopene has been recently proposed to play a critical role on anticarcinogenic action at different levels. The photoprotective properties of lycopene remain contradictory. Some studies point out a positive and othe...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.12491
更新日期:2014-12-01 00:00:00
abstract::The proportion of acral melanoma (AM) is much higher in Asian populations than in Caucasian populations. Although mutational profiles associated with AM have been discovered in Caucasian populations, knowledge of its genetic alterations in Asian populations is limited. To describe the molecular nature of AM in Korean ...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13321
更新日期:2017-10-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2008.00719.x
更新日期:2008-10-01 00:00:00
abstract::Chronic pruritus of any origin is a frequent discomfort in daily medical practice, and its therapy is challenging. Frequently, the underlying origin may not be identified and symptomatic therapy is necessary. Conventional treatment modalities such as antihistamines often lack efficacy, and hence new therapeutic strate...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2007.00664.x
更新日期:2008-03-01 00:00:00