Abstract:
:As global transcriptome analyses with a growing demand on layer-specific applications are widely used in cutaneous biology, we investigated the effect of established and optimized dermo-epidermal separation methods on the quality of RNA. We compared enzymatic separation with dispase, chemical separation with 1 M sodium chloride and heat separation to a treatment with 3.8% ammonium thioyanate. The impact of freezing as well as the addition of 10 mM aurintricarboxylic acid was considered in the evaluation of the amount and quality of isolated RNA from dermis and epidermis. Using the low abundant gene kallikrein 12 for real-time PCR analysis, we were able to demonstrate the superior RNA quality after dermo-epidermal separation using 3.8% ammonium thiocyanate. In addition to the time effectiveness this separation technique promises dermal and epidermal purity and is therefore the method of choice for producing high-quality RNA for genome-wide dermal and epidermal transcriptional analysis.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Trost A,Bauer JW,Lanschützer C,Laimer M,Emberger M,Hintner H,Onder Kdoi
10.1111/j.1600-0625.2006.00534.xsubject
Has Abstractpub_date
2007-03-01 00:00:00pages
185-90issue
3eissn
0906-6705issn
1600-0625pii
EXD534journal_volume
16pub_type
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journal_title:Experimental dermatology
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 信件
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
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更新日期:2014-09-01 00:00:00
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pub_type: 评论,杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2003.00019.x
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/exd.12422
更新日期:2014-06-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.0906-6705.2005.00317.x
更新日期:2005-07-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12285
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.2001.100107.x
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pub_type: 信件
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pub_type: 评论,杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
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