Abstract:
:We have previously demonstrated that serum of patients with pemphigus vulgaris induces reduction of desmoglein 3 (Dsg3) half-life in keratinocytes (FEBS Lett 2006: 580: 3276). This phenomenon seems to occur as a consequence of the progressive depletion of Dsg3 from desmosomes. Here we reported that reduction of full-length Dsg3 may be due to its progressive cleavage, leading to the formation of two fragmentation products with apparent molecular masses of about 60 kDa (fragment 1) and 70 kDa (fragment 2), as revealed by Western blotting. Unexpectedly, analysis of fragmentation pattern suggested cleavage to occur intracellularly. Consistently, fragment 1 was shed and localized within the cytosol, as shown by living cell immunofluorescence microscopy. Total amounts of full-length plakoglobin and Dsg1 were apparently unchanged. Taken together, our findings provide evidence that proteolytic processing of Dsg3 can lead to depletion of Dsg3 from the cell.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Cirillo N,Campisi G,Gombos F,Perillo L,Femiano F,Lanza Adoi
10.1111/j.1600-0625.2008.00719.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
858-63issue
10eissn
0906-6705issn
1600-0625pii
EXD719journal_volume
17pub_type
杂志文章abstract::The proliferative responses of cells to mitogens decrease during aging, and this may result from age-related defects in signal transduction in response to mitogens. In this study, we have investigated the age-related alteration of responses to epidermal growth factor (EGF) in cultured human keratinocytes that were sen...
journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
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abstract::Alopecia areata (AA) is an autoimmune disease that results in spot baldness in humans. Adequate animal models for AA are currently lacking. The objective of this study was to elucidate the mechanism of autoimmune-like alopecia (ALA) in C57BL/6.CD80CD86-deficient (B6.CD80CD86-/- ) mice. Incidence and severity of alopec...
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2008.00823.x
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 信件
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journal_title:Experimental dermatology
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