Abstract:
:Keratinocytes (KCs) play a critical role in maintaining the cutaneous structure and are involved in various physiological and pathologic processes of the skin. Many inflammatory skin diseases and skin cancers result from excessive proliferation and insufficient apoptosis of KCs. Recent data suggested that the sonic hedgehog (Shh) signalling pathway plays an essential role in the proliferation and apoptosis of normal KCs. However, the mechanism remains poorly defined. Here, we provide evidence that Shh signalling induces proliferation and inhibits apoptosis in normal KCs via cyclin D1 and Bcl2 in an extracellular signal-regulatedkinase (MEK)/extracellular signal-regulated kinase (ERK)-dependent manner. In addition, the effect is independent of phosphoinositide-3 kinase (PI3K)/AKT or Janus kinase/signal transducer and activator of transcription (JAK/STAT) 1/3 pathways. Furthermore, we observed that epidermal growth factor receptor (EGFR) signalling modulates the activity of Shh signalling pathway; besides, Shh and EGFR signalling act additively to induce the ERK activation and the increases in cyclin D1 and Bcl2 thereby affecting proliferation and apoptosis in KCs in vitro. The present study suggests that the MEK/ERK1/2 activation is part of the mechanism of Shh signal-mediated proliferation and apoptosis in normal KCs. Our results may help to elucidate the regulatory mechanisms of the Shh pathway in normal KCs and the pathogenesis of related skin disorders.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Liu H,Jian Q,Xue K,Ma C,Xie F,Wang R,Liao W,Liu Y,Chi S,Li Cdoi
10.1111/exd.12556subject
Has Abstractpub_date
2014-12-01 00:00:00pages
896-901issue
12eissn
0906-6705issn
1600-0625journal_volume
23pub_type
杂志文章abstract::Ultraviolet radiation (UVR) is known to be involved in the initiation and progression of malignant melanoma. Many studies have focused on the initiation of melanoma, but less is known about the effect of UVR on established tumor cells. Here, we show that after ultraviolet-B (UVB) irradiation, melanoma cells (MM) are a...
journal_title:Experimental dermatology
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pub_type: 信件
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journal_title:Experimental dermatology
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章,评审
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13555
更新日期:2018-07-01 00:00:00
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pub_type: 杂志文章
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doi:10.1111/exd.14093
更新日期:2020-05-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2009.00999.x
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 信件
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journal_title:Experimental dermatology
pub_type: 杂志文章
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