Abstract:
:Potentially lethal damage (PLD) repair has been defined as that property conferring the ability of cells to recover from DNA damage depending on the postirradiation environment. Using a novel cyclin dependent kinase 1 inhibitor RO-3306 to arrest cells in the G2 phase of the cell cycle, examined PLD repair in G2 in cultured Chinese hamster ovary (CHO) cells. Several CHO-derived DNA repair mutant cell lines were used in this study to elucidate the mechanism of DNA double-strand break repair and to examine PLD repair during the G2 phase of the cell cycle. While arrested in G2 phase, wild-type CHO cells displayed significant PLD repair and improved cell survival compared with cells released immediately from G2 after irradiation. Both the radiation-induced chromosomal aberrations and the delayed entry into mitosis were also reduced by G2-holding PLD recovery. The PLD repair observed in G2 was observed in nonhomologous end-joining (NHEJ) mutant cell lines but absent in homologous recombination mutant cell lines. From the survival curves, G2-NHEJ mutant cell lines were found to be very sensitive to gamma-ray exposure when compared to G2/homologous recombination mutant cell lines. Our findings suggest that after exposure to ionizing radiation during G2, NHEJ is responsible for the majority of non-PLD repair, and conversely, that the homologous recombination is responsible for PLD repair in G2.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Maeda J,Bell JJ,Genet SC,Fujii Y,Genet MD,Brents CA,Genik PC,Kato TAdoi
10.1667/RR13744.1subject
Has Abstractpub_date
2014-10-01 00:00:00pages
448-57issue
4eissn
0033-7587issn
1938-5404journal_volume
182pub_type
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journal_title:Radiation research
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journal_title:Radiation research
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journal_title:Radiation research
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journal_title:Radiation research
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更新日期:1995-05-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-06-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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更新日期:1983-05-01 00:00:00
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更新日期:2005-02-01 00:00:00
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journal_title:Radiation research
pub_type: 杂志文章
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journal_title:Radiation research
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journal_title:Radiation research
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