Abstract:
OBJECTIVES:Most developmental processes are under the control of small regulatory RNAs called microRNAs (miRNAs). We hypothesize that different fetal developmental processes might be reflected by extracellular miRNAs in maternal plasma and may be utilized as biomarkers for the noninvasive prenatal diagnosis of chromosomal aneuploidies. In this proof-of-concept study, we report on the identification of extracellular miRNAs in maternal plasma of Down syndrome (DS) pregnancies. METHODS:Using high-throughput quantitative PCR (HT-qPCR), 1043 miRNAs were investigated in maternal plasma via comparison of seven DS pregnancies with age and fetal sex matched controls. RESULTS:Six hundred and ninety-five miRNAs were identified. Thirty-six significantly differentially expressed mature miRNAs were identified as potential biomarkers. Hierarchical cluster analysis of these miRNAs resulted in the clear discrimination of DS from euploid pregnancies. Gene targets of the differentially expressed miRNAs were enriched in signaling pathways such as mucin type-O-glycans, ECM-receptor interactions, TGF-beta, and endocytosis, which have been previously associated with DS. CONCLUSIONS:miRNAs are promising and stable biomarkers for a broad range of diseases and may allow a reliable, cost-efficient diagnostic tool for the noninvasive prenatal diagnosis of DS.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Kamhieh-Milz J,Moftah RF,Bal G,Futschik M,Sterzer V,Khorramshahi O,Burow M,Thiel G,Stuke-Sontheimer A,Chaoui R,Kamhieh-Milz S,Salama Adoi
10.1155/2014/402475subject
Has Abstractpub_date
2014-01-01 00:00:00pages
402475eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract:OBJECTIVE:Greater skeletal muscle fat infiltration occurs with age and contributes to numerous negative health outcomes. The primary purpose was to determine whether intermuscular adipose tissue (IMAT) can be influenced by an exercise intervention and if a greater reduction in IMAT occurs with eccentric versus traditio...
journal_title:BioMed research international
pub_type: 杂志文章,随机对照试验
doi:10.1155/2014/398960
更新日期:2014-01-01 00:00:00
abstract::Feature selection has become the essential step in biomarker discovery from high-dimensional genomics data. It is recognized that different feature selection techniques may result in different set of biomarkers, that is, different groups of genes highly correlated to a given pathological condition, but few direct comp...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/387673
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/8284363
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/930925
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2020-05-25 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/175025
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/485783
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/482093
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/239164
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/5171242
更新日期:2020-10-19 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/580849
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/309631
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
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更新日期:2015-01-01 00:00:00
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doi:10.1155/2019/3945475
更新日期:2019-07-04 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/196837
更新日期:2014-01-01 00:00:00
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doi:10.1155/2020/4827045
更新日期:2020-06-17 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/539348
更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/5484731
更新日期:2018-07-18 00:00:00