Abstract:
OBJECTIVES:Most developmental processes are under the control of small regulatory RNAs called microRNAs (miRNAs). We hypothesize that different fetal developmental processes might be reflected by extracellular miRNAs in maternal plasma and may be utilized as biomarkers for the noninvasive prenatal diagnosis of chromosomal aneuploidies. In this proof-of-concept study, we report on the identification of extracellular miRNAs in maternal plasma of Down syndrome (DS) pregnancies. METHODS:Using high-throughput quantitative PCR (HT-qPCR), 1043 miRNAs were investigated in maternal plasma via comparison of seven DS pregnancies with age and fetal sex matched controls. RESULTS:Six hundred and ninety-five miRNAs were identified. Thirty-six significantly differentially expressed mature miRNAs were identified as potential biomarkers. Hierarchical cluster analysis of these miRNAs resulted in the clear discrimination of DS from euploid pregnancies. Gene targets of the differentially expressed miRNAs were enriched in signaling pathways such as mucin type-O-glycans, ECM-receptor interactions, TGF-beta, and endocytosis, which have been previously associated with DS. CONCLUSIONS:miRNAs are promising and stable biomarkers for a broad range of diseases and may allow a reliable, cost-efficient diagnostic tool for the noninvasive prenatal diagnosis of DS.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Kamhieh-Milz J,Moftah RF,Bal G,Futschik M,Sterzer V,Khorramshahi O,Burow M,Thiel G,Stuke-Sontheimer A,Chaoui R,Kamhieh-Milz S,Salama Adoi
10.1155/2014/402475subject
Has Abstractpub_date
2014-01-01 00:00:00pages
402475eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/3764032
更新日期:2018-09-24 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1375085
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
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journal_title:BioMed research international
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2017/4569698
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/717261
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journal_title:BioMed research international
pub_type: 历史文章,杂志文章
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doi:10.1155/2015/673018
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 历史文章,杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/919374
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章,多中心研究
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2019-06-17 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/803914
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/3895834
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/790842
更新日期:2013-01-01 00:00:00