Abstract:
:The present study aimed to investigate the effects of the WW domain-containing oxidoreductase (WWOX) gene on the stem cell properties of human ovarian cancer stem cells. A eukaryotic expression vector containing the WWOX gene was transfected into human ovarian cancer stem cells and Western blotting was used to assess the expression of WWOX protein in the transfected cells compared with the control cells (untransfected cells and cells transfected with the empty vector). The self-renewal abilities of these three types of stem cells was also assessed in vitro. To monitor changes in their differentiation potential, cells were cultured in medium supplemented with serum, and the expression of specific stem cell markers was determined. Drug-sensitivity tests were used to measure the sensitivity of the stem cells to cisplatin, doxorubicin, and mitoxantrone. The cells were also transplanted into non-obese diabetic (NOD)/severe combined immunodeficiency (SCID) mice to determine the changes in their tumorigenicity in vivo. Cells transfected with the WWOX-expressing plasmid stably expressed WWOX protein, while no WWOX protein was detected in control cells. Compared with the two types of control cells, WWOX-expressing stem cells manifested significantly reduced self-renewal ability. Compared with control cells, the expression levels of stem cell markers, including CD133, CD117, ATP-binding cassette sub-family G member 2, Nanog, octamer-binding transcription factor 4 and breast cancer resistance protein, were significantly lower in WWOX-expressing cells, while the level of the differentiation marker E-cadherin was significantly higher in WWOX-expressing cells. Furthermore, WWOX-expressing cells were more sensitive to treatment with cisplatin, doxorubicin and mitoxantrone. In NOD/SCID mice, the tumorigenicity of WWOX-expressing cells was significantly lower compared with that of control cells. The results indicate that the tumor suppressor WWOX suppresses stem cell properties in cancer stem cells, including self-renewal ability, differentiation potential, in vivo tumorigenic capability, high-level expression of stem cell genes and multidrug resistance.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Yan HC,Xu J,Fang LS,Qiu YY,Lin XM,Huang HX,Han QYdoi
10.3892/ol.2015.2971subject
Has Abstractpub_date
2015-04-01 00:00:00pages
1614-1620issue
4eissn
1792-1074issn
1792-1082pii
ol-09-04-1614journal_volume
9pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Colonic schwannomas are rare gastrointestinal mesenchymal tumors, and only a limited number of cases has been reported. The occurrence of these tumors is less common in the large intestine than in the stomach. The present study reports a case of colonic schwannoma in a 62-year-old female patient with no specific sympt...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4271
更新日期:2016-04-01 00:00:00
abstract::Fibroadenomas are a type of benign tumor that occur in young women below the age of 35 years old. The tumors are the second most common type of tumor after fibrocystic disease. The chance of carcinoma arising in a fibroadenoma is extremely low. To date, <130 such cases have been reported. Previous studies have reporte...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3420
更新日期:2015-09-01 00:00:00
abstract::The present study aimed to identify microRNA (miRNA) expression profiles associated with brain metastases of colorectal cancers. We conducted miRNA expression profiling of 3 primary colorectal cancers and 3 brain-metastatic carcinomas using Agilent Human miRNA Microarrays. Quantitative real-time reverse transcription-...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.497
更新日期:2012-02-01 00:00:00
abstract::Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to ex...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2562
更新日期:2014-12-01 00:00:00
abstract::Abnormalities in proto-oncogene B-Raf (BRAF) are typical in several subgroups of gliomas, including pilocytic astrocytomas, optic nerve gliomas, pleomorphic xanthoastrocytomas (PXA), anaplastic PXAs and gangliogliomas. However, they are rarely reported in adult gliomas. BRAF alterations are frequent in a distinct vari...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8919
更新日期:2018-08-01 00:00:00
abstract::Lung cancer is one of the most lethal types of cancer, and its poor prognosis is primarily due to drug resistance and cancer recurrence. As it is associated with a low five-year survival rate, lung cancer stem cells (LCSCs) have been the subject of numerous recent studies. For these studies of LCSCs, lung cancer cell ...
journal_title:Oncology letters
pub_type: 杂志文章,评审
doi:10.3892/ol.2018.8265
更新日期:2018-05-01 00:00:00
abstract::microRNAs (miRNAs) are important regulators in the development and progression of human gastric cancer (GC). However, the expression and biological function of miR-449c in GC remains unknown. In the present study, miR-449c was demonstrated to be downregulated in GC tissues and cell lines compared with normal. Decrease...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8609
更新日期:2018-07-01 00:00:00
abstract::Icotinib is an epidermal growth factor receptor tyrosine kinase inhibitor, which has been revealed to inhibit proliferation in tumor cells. However, the effect of icotinib on cancer cell metastasis remains to be explained. This study examines the effect of icotinib on the migration and invasion of squamous cells of to...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2311
更新日期:2014-09-01 00:00:00
abstract::Numerous studies concerning hepatic arterial infusion chemotherapy (HAIC) have been conducted by adopting regimens containing 5-fluorouracil (FU), with a favourable efficacy compared with conventional transcatheter arterial chemoembolisation (TACE) treatment; however, the detailed mechanism of HAIC remains unclear. Th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8242
更新日期:2018-05-01 00:00:00
abstract::As an important chemotherapeutic agent for the treatment of osteosarcoma, the effectiveness of cisplatin is considered to be due to its unique properties, which allow it to penetrate the cell membrane and form various DNA-platinum adducts, resulting in genetic alterations or DNA damage. However, chemoresistance to cis...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7432
更新日期:2018-01-01 00:00:00
abstract::HRas proto-oncogene (HRAS) is one of the most commonly mutated genes in thyroid cancer, with mutations frequently occurring in the follicular and Hurthle cell subtypes. However, the contribution of mutations in HRAS to papillary thyroid carcinoma (PTC) progression and the tall-cell variant (TCV) is poorly understood. ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7818
更新日期:2018-04-01 00:00:00
abstract::Hyperbaric oxygenation (HBO) on postoperative rehabilitation of brain tumors and effects on tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were explored. A retrospective analysis of 132 patients with brain tumors treated in the People's Hospital of Rizhao from October 2014 to October 2017 was performe...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10000
更新日期:2019-03-01 00:00:00
abstract::The study aim was to evaluate the association of the expression of serum microribonucleic acid-590 (miR-590) with the risk of lung squamous cell carcinoma (LUSC), clinicopathological staging and prognosis. A total of 237 patients with LUSC and 100 healthy volunteers (control group) were included in the study. Total RN...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7497
更新日期:2018-02-01 00:00:00
abstract::It has been demonstrated that microRNA-497 (miR-497) acts as a tumor suppressor and is involved in tumor progression, development and metastasis in several types of cancer. However, little is known about the exact role of miR-497 in osteosarcoma (OS). The aim of the current study was to investigate the potential role ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4162
更新日期:2016-03-01 00:00:00
abstract::The present study aimed to analyze the imaging features and pathological basis of primary hepatic neuroendocrine carcinoma (PHNEC). A retrospective analysis of the imaging and pathological features of nine PHNEC cases was carried out at The Second Xiangya Hospital of Central South University (Changsha, China). The nin...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1844
更新日期:2014-04-01 00:00:00
abstract::Survivin is expressed in the nucleus and/or cytoplasm of various malignant cells. Nuclear survivin is critical for the completion of mitosis, while cytoplasmic survivin functions as an inhibitor of apoptosis. The expression of survivin has been reported to be associated with the aggressiveness of certain types of canc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3374
更新日期:2015-09-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the leading digestive malignancies, with a high metastasis and recurrence. The development and rapid progression of HCC involves numerous complex molecular and cellular events. Therefore, developing effective methods for the prevention and treatment of HCC requires an improved ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6369
更新日期:2017-08-01 00:00:00
abstract::In the current era of multi-disciplinary treatment, precise and detailed diagnosis prior to treatment is crucial for clinical practice. For different lesions that fit different indications, the optimum approach for treatment differs significantly. Thus, the recent 8th American Joint Committee on Cancer classification ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.9920
更新日期:2019-03-01 00:00:00
abstract::Forkhead box M1 (FOXM1) and hedgehog (Hh) signaling pathway are implicated in the formation and development of human tumors, including cervical cancer. Previous studies have indicated that FOXM1 may be a downstream target gene of the Hh signaling pathway, but their association in cervical cancer is largely unknown. In...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4932
更新日期:2016-10-01 00:00:00
abstract::The aim of the current study was to investigate the effects of resveratrol (Res) on vascular endothelial growth factor (VEGF) expression and cell proliferation in the human osteosarcoma cell line U20S. U20S cells were treated with Res at various concentrations (0, 10, 20 and 40 μmol/l) for various times (24, 48 and 72...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.824
更新日期:2012-10-01 00:00:00
abstract::The present study aimed to investigate the imaging characteristics of primary malignant bone tumors in children. The imaging results of 34 children with primary malignant bone tumors confirmed by histopathological diagnosis between March 2008 and January 2014 were retrospectively analyzed. In total, 25 patients had os...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6939
更新日期:2017-11-01 00:00:00
abstract::Metastasis-associated in colon cancer-1 (MACC1) is a gene that has been newly identified by a genome-wide search for differentially expressed genes in human colon cancer tissues, metastases and normal tissues. MACC1 exerts an important role in colon cancer metastasis through upregulation of the c-Met proto-oncogene. T...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2984
更新日期:2015-05-01 00:00:00
abstract::MicroRNA (MiR)-506 serves a vital role in several types of cancer. However, the role of miR-506 in bladder cancer (BCa) progression remains to be investigated. The present study demonstrated that miR-506 expression was downregulated in BCa tissues and cell lines. Meanwhile, overexpression of miR-506 inhibited cell pro...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9594
更新日期:2019-01-01 00:00:00
abstract::The risk factors for the development of hepatocellular carcinoma (HCC) in patients who have achieved a long-term sustained viral response (SVR) to interferon (IFN) are not fully understood. This study aimed to investigate the characteristics of patients who developed HCC after 10 years of achieving SVR. We retrospecti...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000075
更新日期:2010-05-01 00:00:00
abstract::The epidermal growth factor receptor (EGF-R) signaling pathway is thought to have an important role in the development and progression of several carcinomas, as it is associated with cell proliferation, differentiation and migration. Activation of EGF-R signaling regulates epithelial-mesenchymal transition (EMT)-assoc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5406
更新日期:2017-01-01 00:00:00
abstract::Previous studies have demonstrated that C-X-C chemokine receptor type 7 (CXCR7) regulates papillary thyroid carcinoma (PTC) growth and metastasis; however, the molecular mechanisms underlying this regulation remain unclear. In the present study, the protein expression profiles of the PTC cell line GLAG-66 and GLAG-66 ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6574
更新日期:2017-09-01 00:00:00
abstract::Approximately 34-86% of neoplasms of the salivary glands are located in the parotid gland and parotidectomy is the first-line treatment for parotid gland tumors. Frey syndrome and cosmetic deformity are common complications experienced by patients following parotidectomy and the sternocleidomastoid muscle flap (SCMF) ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1179
更新日期:2013-04-01 00:00:00
abstract::For osteoblastoma, with its predilection for the spinal column and appendicular skeleton, the skull is an unusual site, and paranasal sinus involvement is very rare. Herein, we report on a case in which the disease was located within the sphenoid bone. To the best of our knowledge, this is the 4th reported case of ost...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000065
更新日期:2010-03-01 00:00:00
abstract::Cardiac toxicity has been the major concern when using doxorubicin (DOX) in cancer therapy. Thrombopoietin (TPO) protects cardiac cells from DOX-induced cell damage; however, its molecular mechanism remains exclusive. The anti-autophagic and anti-apoptotic effects of TPO upon DOX treatment were studied in the cardiac ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7410
更新日期:2018-01-01 00:00:00
abstract::Based on our previous analysis of neutral glycolipids in the human endometrium, the present authors already reported that the concentrations of glucosylceramide, lactosylceramide and globotriaosylceramide (Gb3Cer), in which both fatty acids and sphingosines in the ceramides are hydroxylated, exhibit a marked increase ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5410
更新日期:2017-01-01 00:00:00