Abstract:
:Pythiosis is a life-threatening disease caused by the fungus-like microorganism Pythium insidiosum that can lead to death if not treated. Since P. insidiosum has particular cell wall characteristics, pythiosis is difficult to treat, as it does not respond well to traditional antifungal drugs. In our study, we investigated a new immunotherapeutic approach with potential use in treatment and in the acquisition of immunity against pythiosis. Dendritic cells from both human and mouse, pulsed with P. insidiosum heat-inactivated zoospore, (1,3)(1,6)-β-glucan and the immunotherapeutic PitiumVac® efficiently induced naïve T cell differentiation in a Th1 phenotype by the activation of specific Th1 cytokine production in vitro. Heat-inactivated zoospores showed the greatest Th1 response among the tested groups, with a significant increase in IL-6 and IFN-γ production in human cells. In mice cells, we also observed a Th17 pathway induction, with an increase on the IL-17A levels in lymphocytes cultured with β-glucan pulsed DCs. These results suggest a potential use of DCs pulsed with P. insidiosum antigens as a new therapeutic strategy in the treatment and acquisition of immunity against pythiosis.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Ledur PC,Tondolo JSM,Jesus FPK,Verdi CM,Loreto ÉS,Alves SH,Santurio JMdoi
10.1016/j.imbio.2017.10.033subject
Has Abstractpub_date
2018-03-01 00:00:00pages
294-299issue
3eissn
0171-2985issn
1878-3279pii
S0171-2985(17)30174-2journal_volume
223pub_type
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