Abstract:
AIM:To study the antiinflammatory effect of lidocaine in intestinal epithelial cells. METHODS:HT-29 and T-84 cells were grown in culture with and without TNF-alpha, lidocaine, aconitine and veratridine. The secretion of IL-8 and IP-10 was measured by ELISA. A cDNA microarray was used to assess gene expression. Real-time PCR was used to confirm the results. Western blots and a modified electromobility shift assay (EMSA) were used to assess NFkappaB activation. RESULTS:Lidocaine inhibited spontaneous and TNF-alpha induced secretion of IL-8 and IP-10. The combination of veratridine or aconitine, voltage-gated sodium channels (VGSC) agonists that open VGSCs, with lidocaine did not alter the effect of lidocaine on cytokine secretion. Gene array analysis revealed that IkappaB transcription was induced by TNF-alpha and inhibited by lidocaine. IkappaB real-time PCR confirmed this observation. A Western blot analysis demonstrated that the degradation of IkappaB following TNF-alpha treatment was markedly inhibited by lidocaine. Lidocaine treatment resulted in decreased generation of phosphorylated IkappaB. A modified EMSA was complementary and demonstrated marked inhibition of NFkappaB nuclear binding. CONCLUSION:Lidocaine inhibits IL-8 and IP-10 secretion from intestinal cells. This effect is mediated by inhibition of NFkappaB activation via decreased IkappaB phosphorylation and is not mediated by lidocaine's effect on VGSC.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Lang A,Ben Horin S,Picard O,Fudim E,Amariglio N,Chowers Ydoi
10.1016/j.imbio.2009.05.006subject
Has Abstractpub_date
2010-04-01 00:00:00pages
304-13issue
4eissn
0171-2985issn
1878-3279pii
S0171-2985(09)00087-4journal_volume
215pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::Airway remodeling is a characteristic of bronchial asthma. The process involves the expression of many genes, such as transforming growth factor-beta (TGF-β), tissue inhibitors of metalloproteinases (TIMP-1), MMP and arginase. Human rhinovirus (HRV) is known to cause asthma exacerbations, and viral infections might be...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.05.010
更新日期:2017-08-01 00:00:00
abstract::A long-lasting skin tolerance to non-H-2 alloantigens has been reported to be readily induced, when the recipient mice were primed intravenously (i.v.) with 5 x 10(7) spleen cells (SC) plus 1.5 x 10(7) bone marrow cells (BMC) from the H-2 identical strains of mice, and treated intraperitoneally (i.p.) with 200 mg/kg C...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80511-5
更新日期:1990-11-01 00:00:00
abstract::CD147 is a broadly expressed cell surface molecule of the immunoglobulin superfamily whose expression is up-regulated upon T cell activation. Engagement of CD147 by CD147 monoclonal antibodies (mAbs) has been shown to induce homotypic aggregation of U937 cells. To study intracellular signal transduction induced by the...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(01)80015-2
更新日期:2001-05-01 00:00:00
abstract::Defects in macrophage function have been implicated in the establishment of Crohn's disease (CD). However, the response of macrophages from CD patients to live bacteria, particularly Mycobacterium avium subsp. paratuberculosis (MAP), has not been addressed. Considering MAP has long been associated to CD, our objective...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.01.002
更新日期:2011-08-01 00:00:00
abstract::Cholesterol crystals are known to be a hallmark of atherosclerosis with recent studies demonstrating deposition of these crystals in early fatty streak formation as well as penetrating the intima following plaque rupture. Inflammation has also become a central focus in atheroma development and endothelial cell activat...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.06.006
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:T helper cells can differentiate into several subsets of T lymphocytes, including Th1, Th2, and regulatory T (Treg) cells. As a result of this ability to differentiate, the corresponding T cell receptor (TCR) spectra display considerable cellular plasticity and interchangeability. In contrast, T lymphocyte d...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.02.007
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubate...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2016.11.004
更新日期:2017-03-01 00:00:00
abstract::Leukocytes found at the human maternal-fetal interface participate in the inflammatory process associated with both preterm and term labor; therein, effective methods for their isolation that allow further phenotypic and functional analyses are necessary. Leukocyte isolation is usually carried out through scraping or ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2018.09.001
更新日期:2019-01-01 00:00:00
abstract::Macrophage functions in the immune response depend on their ability to infiltrate tissues and organs. The penetration between and within the tissues requires degradation of extracellular matrix (ECM), a function performed by the specialized, endopeptidase- and actin filament- rich organelles located at the ventral sur...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2018.07.009
更新日期:2018-11-01 00:00:00
abstract::Considering that imbalance of central tolerance in the thymus contributes to aggressive autoimmunity, we compared the expression of peripheral tissue autoantigens (PTA) genes, which are involved in self-representation in the thymic stroma, of two mouse strains; DBA-1/J (MHC-H2(q)) susceptible and DBA-2/J (MHC-H2(d)) r...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.09.007
更新日期:2011-05-01 00:00:00
abstract::The activating C-type lectin-like receptor NKG2D, which is expressed by mouse NK cells and activated CD8 T cells, was previously demonstrated to be involved in tumor rejection and as a defense mechanism against viral and bacterial infections. Because CD8 T cells are important for protective immune responses during chr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.09.010
更新日期:2011-05-01 00:00:00
abstract::Human T-lymphocyte clones showing proliferative and cytotoxic responses specific for HLA antigens were isolated. To obtain enriched numbers of functionally active colonies, the cells were first sensitized in vitro in a tertiary mixed lymphocyte culture (MLC), using 10 stimulating cells for each responding cell. To ass...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(81)80062-9
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVE:To investigate the close association between different antiphospholipid antibodies (aPL) caused by infection and their appearance together with a prolonged activated partial thromboplastin time (aPTT). METHODS:Sera from 122 children were evaluated in this study. Thirty-seven children with mild to medium prol...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2005.10.012
更新日期:2005-01-01 00:00:00
abstract::The phagocyte pattern recognition receptor Toll-like receptor 2 (TLR2) and the multi-receptor adaptor MyD88 contribute to the reduction of bacterial load in infections with intra- and extra-cellular Gram-positive bacteria. Their mechanism of antibacterial action is mostly unresolved but evident in vivo by an increased...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2005.05.017
更新日期:2005-01-01 00:00:00
abstract::The mechanisms of tolerance induction occurring in the course of allergen-specific immunotherapy have not been elucidated in full detail. Our study aimed to characterize high zone tolerance in mouse models of type I allergy and of allergic airway inflammation induced by subcutaneous sensitization of mice with high dos...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.10.022
更新日期:2015-04-01 00:00:00
abstract::Mycoplasma arthritidis produces a so-far only partially characterized soluble material (MAS) that has a potent mitogenic effect on T lymphocytes of several species. Similar to staphylococcal enterotoxins and a number of related toxins secreted by other species of bacteria, nanogram quantities of these so-called supera...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80254-8
更新日期:1992-11-01 00:00:00
abstract::Chemokine CCL21 can effectively attract CCR7(+) dendritic cells (DCs), however its role in this event is poorly understood. In this report, we investigated the effect of exogenous CCL21 expressed in breast cancer MCF-7 on human monocyte-derived DCs. CCL21-transfected MCF-7 stimulation prompted DC functions: migration,...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2007.10.003
更新日期:2008-01-01 00:00:00
abstract::Ribosomal 30S and 50S subunits as well as total proteins from the large subunit of E. coli ribosomes were found to be potent mitogens for both human adult and cord blood lymphocytes. On the other hand, the RNA fraction of the 50S subunit had only a weak stimulatory capacity suggesting that the protein moiety contains ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(80)80079-9
更新日期:1980-01-01 00:00:00
abstract::Respiratory viral infections have been implicated in the origin of, protection from and exacerbation of allergy-related symptoms in a variety of ways. Viral infections are closely linked to infantile wheezing. Severe bronchiolitis in early infancy may predispose to chronic childhood asthma as well as allergic sensitiz...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2007.03.008
更新日期:2007-01-01 00:00:00
abstract::BALB/c mice injected with 0.2 mg TNP-ovalbumin (OA) within 24 hours after birth showed reduced levels of functionally active TNP-specific B cells and, accordingly, of plaque-forming cells (PFC) after challenge with carrier-bound TNP until the age of 8 weeks. Yet, when B cells of tolerized mice were cultured in the abs...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80105-5
更新日期:1987-12-01 00:00:00
abstract::Kinetic methods can provide significant information concerning the mechanism of cellular cytotoxicity reactions. Previous kinetic studies of cytotoxic T lymphocytes (Tc) have been hampered by the heterogeneity of the effector cell population tested. We therefore examined the kinetics of lysis mediated by cloned, IL 2 ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(88)80065-2
更新日期:1988-12-01 00:00:00
abstract::Gut microbiota and probiotic strains play an important role in oral tolerance by modulating regulatory and effector cell components of the immune system. We have previously described the ability of Lactobacilli to influence both the innate and adaptive immunity to wheat gluten, a food antigen, in mouse. In this study,...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.01.004
更新日期:2015-06-01 00:00:00
abstract::Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the pro...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.04.009
更新日期:2011-10-01 00:00:00
abstract::The enhancing effects of the complement system for humoral immunity have primarily focused upon the recognition of complement-bound foreign antigens by a co-receptor complex of the antigen-specific B cell receptor (BCR) and complement receptor 2 (Cr2). In vivo experiments using Cr2 gene deficient mice (which lack the ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2013.08.003
更新日期:2014-01-01 00:00:00
abstract::OK-432, a streptococcal preparation, has been shown as an effective activator to induce human monocyte-derived dendritic cells maturation. During this process, the activation of Toll-like receptor 4 plays an important role. However, the signaling pathway involved in has not been fully understood. In the present study,...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2008.10.008
更新日期:2009-01-01 00:00:00
abstract::IgE-mediated food allergies are an important cause of life-threatening hypersensitivity reactions. Orally administered peanut antigens mixed with the mucosal adjuvant cholera toxin (CT) induce a strong peanut extract (PE)-specific serum IgE response that is correlated with T-helper type 1 (Th1) and type 2 (Th2)-like T...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.02.004
更新日期:2010-12-01 00:00:00
abstract::When monocytes are stimulated with LPS (lipopolysaccharide) repeatedly then the initially high expression of the TNF (tumor necrosis factor) gene is only very low, i.e. the cells are tolerant to LPS. Tolerant cells still express the CD14 receptor and they can still be activated to mobilize NF-kappa B into nucleus. Ana...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(97)80029-0
更新日期:1997-12-01 00:00:00
abstract::Individuals infected with Strongyloides stercoralis have been reported to produce different immunoglobulins isotypes, yet few studies have evaluated their use in strongyloidiasis diagnosis. The aim of this work was to evaluate the immunoreactivity of different classes and subclasses of anti-S. stercoralis circulating ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.151898
更新日期:2020-03-01 00:00:00
abstract::Peritoneal exudate T lymphocytes (PETLs) that mediate delayed-type hypersensitivity (DTH) to sheep red blood cells in mice were modified by in vitro treatment methods which modify surface carbohydrate constituents. Neuraminidase treatment resulted in the release of both N-acetylneuraminic acid and N-glycolyl-neuramini...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(81)80046-0
更新日期:1981-01-01 00:00:00
abstract::The prevailing paradigm defines sarcoidosis as a disease of unknown etiology characterized by a systemic noncaseating epithelioid granulomatous response (SGR). This formulation fails to account for the elusiveness of the etiological agent, the nature of the Kveim response, the paradox of cutaneous delayed type hyperse...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2016.12.005
更新日期:2017-04-01 00:00:00