Abstract:
:Leukocytes found at the human maternal-fetal interface participate in the inflammatory process associated with both preterm and term labor; therein, effective methods for their isolation that allow further phenotypic and functional analyses are necessary. Leukocyte isolation is usually carried out through scraping or enzyme digestion of the choriodecidua, however both methods usually limit the use of downstream immunophenotyping or transcriptomic techniques. Here we describe an isolation method based on gentle trypsin digestion that yields a leukocyte-enriched cell mixture with high lymphocyte viability, although less viable myeloid cells. We show that the method does not compromise cell surface markers since isolated leukocytes are suitable for flow cytometry; and that high quality RNA can be obtained from these cells for qRT-PCR and microarray analyses.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
MacDonald-Ramos K,Arenas-Hernandez M,Mancilla-Herrera I,Rangel-Escareño C,Vega-Sanchez Rdoi
10.1016/j.imbio.2018.09.001subject
Has Abstractpub_date
2019-01-01 00:00:00pages
177-181issue
1eissn
0171-2985issn
1878-3279pii
S0171-2985(18)30128-1journal_volume
224pub_type
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