Abstract：
BACKGROUND/AIM:The oxazaphosphorines, ifosfamide and cyclophosphamide, represent a class of alkylating agents. The aim of the present in vitro study was to compare antileukemic activity of 4-hydroperoxyifosfamide (4-OOH-IF) and 4-hydroperoxycyclophosphamide (4-OOH-CP). MATERIALS AND METHODS:The experiments were performed on MOLT-4 and ML-1 cells. The research was conducted using flow cytometry fluorescein diacetate/propidium iodide (PI), fluorescein-conjugated annexin V/PI, CaspGLOW Red Active Caspase-8 and -9, CellEvent™ Caspase-3/7 Green assays, and tetramethylrhodamine ethyl ester test. RESULTS:4-OOH-IF and 4-OOH-CP distinctly reduced cell viability and triggered apoptosis and necrosis, causing changes in intracellular esterase activity, plasma membrane structure and integrity, caspase activation, and mitochondrial membrane potential. The oxazaphosphorines were responsible for the different antileukemic activities. 4-Hydroperoxyifosfamide appeared to be less cytotoxic against the leukemia cells than 4-hydroperoxycyclophosphamide. MOLT-4 cells were more sensitive to the action of the oxazaphosphorines than ML-1 cells. CONCLUSION:The findings provide a new insight on the mechanisms of cytotoxic action of 4-OOH-IF and 4-OOH-CP on the human acute lymphoblastic and myeloblastic leukemia cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Opydo-Chanek M,Śladowska K,Blicharski K,Mikeš J,Fedoročko P,Niemeyer U,Mazur Ldoi
10.21873/anticanres.12088subject
Has Abstractpub_date
2017-11-01 00:00:00pages
6355-6361issue
11eissn
0250-7005issn
1791-7530pii
37/11/6355journal_volume
37pub_type
杂志文章abstract：:The LAI reactivity of a colon organ specific neoantigen (OSN) was recovered from the urine of advanced colon cancer patients. In this study three physico-chemical steps were employed; precipitation by 80% ammonium sulfate, ion exchange, and molecular sieve chromatography. Each isolate was tested for activity and speci...
journal_title：Anticancer research
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更新日期：1986-07-01 00:00:00
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journal_title：Anticancer research
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更新日期：2010-05-01 00:00:00
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更新日期：2005-11-01 00:00:00
abstract：:S-1 is a new oral formulation of 5-fluorouracil (5-FU) containing 1 M tegafur and 0.4 M 5-chloro-2,4-dihydroxypyridine (CDHP) and 1 M potassium oxonate (Oxo). It has been reported to have a high antitumor activity and low gastrointestinal toxicity in rats bearing murine and human tumors. We further studied the possibl...
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pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2015-10-01 00:00:00
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journal_title：Anticancer research
pub_type： 临床试验,杂志文章,随机对照试验
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更新日期：1996-11-01 00:00:00
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doi：
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journal_title：Anticancer research
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更新日期：1999-11-01 00:00:00
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更新日期：2009-07-01 00:00:00
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更新日期：1993-11-01 00:00:00
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更新日期：2009-12-01 00:00:00
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更新日期：1997-07-01 00:00:00
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journal_title：Anticancer research
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