Abstract：
BACKGROUND/AIM:Multidrug resistance (MDR) is often associated with overexpression of P-glycoprotein (ABCB1) in cancer cells. Apatinib is a novel Vascular endothelial growth factor receptor-TKI (VEGFR-TKI) which inhibits the function of ABCB1 in certain cancers. This study aimed to investigate the effect of apatinib on the reversal of paclitaxel (PTX) resistance in A549 lung cancer cells (A549/PTX) and related mechanisms. MATERIALS AND METHODS:A549/PTX cells were treated with apatinib alone, PTX alone, or PTX and apatinib. Cell viability was measured by the CCK8 assay. Apoptosis rate, cell-cycle arrest, Rhodamine efflux and reactive oxygen species (ROS) generation were determined by flow cytometry. The intracellular paclitaxel concentration was measured by ultra performance liquid chromatography (UPLC). Protein levels were analyzed by western blotting. RESULTS:A549/PTX cells had significant resistance to PTX and higher expression of ABCB1 compared to A549 cells. Apatinib increased the cytotoxicity of PTX, enhanced PTX-induced apoptosis and cycle arrest, and triggered intracellular ROS generation in A549/PTX cells. In addition, apatinib treatment increased the concentration of intracellular PTX in A549/PTX cells. Apatinib-PTX combination inhibited AKT and ERK pathways. CONCLUSION:Apatinib reverses the drug resistance to PTX in A549 PTX-resistant cells through inhibiting the function of ABCB1 and resumes anti-cancer effects.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Zhang Q,Song Y,Cheng X,Xu Z,Matthew OA,Wang J,Sun Z,Zhang Xdoi
10.21873/anticanres.13739subject
Has Abstractpub_date
2019-10-01 00:00:00pages
5461-5471issue
10eissn
0250-7005issn
1791-7530pii
39/10/5461journal_volume
39pub_type
杂志文章abstract：BACKGROUND:Several types of human papilloma virus (HPV) have been associated with increased risk for epithelial malignancies. In light of a recently available vaccine that protects against persistent infection by certain HPV types (6, 11, 16, 18) and resulting neoplasias, the prevalence of HPV types was investigated in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
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journal_title：Anticancer research
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:Telomerase activity is associated with most malignant tumors. To evaluate the role of telomerase reactivation as a diagnostic and prognostic marker in oral carcinogenesis activity was investigated in mortal and immortal cell lines in eight oral leukoplakias (OL) and 46 oral squamous cell carcinomas (OSCC). Activity wa...
journal_title：Anticancer research
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更新日期：2001-03-01 00:00:00
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pub_type： 杂志文章
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journal_title：Anticancer research
pub_type： 杂志文章,评审
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更新日期：1998-11-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2003-09-01 00:00:00
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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更新日期：2019-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
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pub_type： 临床试验,杂志文章
doi：
更新日期：2003-11-01 00:00:00
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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doi：
更新日期：1999-01-01 00:00:00
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journal_title：Anticancer research
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journal_title：Anticancer research
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doi：
更新日期：2007-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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doi：
更新日期：2010-09-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2010-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2000-11-01 00:00:00
