Abstract:
:The LAI reactivity of a colon organ specific neoantigen (OSN) was recovered from the urine of advanced colon cancer patients. In this study three physico-chemical steps were employed; precipitation by 80% ammonium sulfate, ion exchange, and molecular sieve chromatography. Each isolate was tested for activity and specificity by the direct tube leukocyte adherence inhibition (LAI) assay employing leukocytes from colon and breast cancer patients. The OSN enriched isolate was then used to generate monoclonal antibodies (Mab). Hybridomas were screened by ELISA. One hybridoma designated Bac 18.1 reacted preferentially with colon OSN and not with the urine from normals or patients with breast cancer. Affinity purification of colon OSN was achieved and it was shown to consist of a single band in SDS-PAGE with an apparent molecular weight of 30,000. The eluted polypeptide was specifically reactive in ELISA as well as in the LAI assay.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Fink A,Harpaz N,Weisman Z,Shani A,Bentwich Zsubject
Has Abstractpub_date
1986-07-01 00:00:00pages
813-7issue
4eissn
0250-7005issn
1791-7530journal_volume
6pub_type
杂志文章abstract:BACKGROUND/AIM:In order to develop an efficient drug-delivery system (DDS), a lipopeptide-loaded liposome that functions as a platform for the transpeptidase reaction mediated by sortase A (SrtA) was constructed and its stability, as well as cell-specific targeting were evaluated in the present study. MATERIALS AND ME...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:The antitumor effect exerted by tumor necrosis factor (TNF) is characteristic in that it causes central necrosis of the tumor mass. Viable tumor cells surrounding the tumor mass remain, however, even after most of the mass is necrotized, and these cells gradually regrow and form tumors. To overcome this, we ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme of 5-fluorouracil (5-FU) catabolism. Several studies have demonstrated the clinical importance of DPD in cancer patients, suggesting that the efficacy of 5-FU may be related to DPD activity in tumor tissue. In the present study, DPD activity a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Eldecalcitol [1α,25-dihydroxy-2β-(3-hydroxypropoxy)vitamin D(3)], an analog of calcitriol (1α,25-dihydroxyvitamin D(3)), possesses a hydroxypropoxy substituent at the 2β-position of calcitriol. Eldecalcitol has potent biological effects on bone disease such as osteoporosis. The marketing of eldecalcitol has very recen...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::To activate the cancerous genetic information in the cell one has either to activate oncogenes or to inactivate antioncogenes (cancerous growth suppressing genes). These processes usually happen in the cell with the aid of external factors, especially chemical carcinogens' and radiations. It was shown with the aid of ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1994-01-01 00:00:00
abstract::Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A phosphate-buffered saline (PBS) survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-06-01 00:00:00
abstract::Calcitriol (1,25-dihydroxyvitamin D3), the active form of vitamin D, promotes growth inhibition and differentiation in prostate cancer (PCa) cells. To unravel the molecular pathways of calcitriol actions, cDNA microarray analysis was used to identify novel calcitriol target genes including two that play key roles in t...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2006-07-01 00:00:00
abstract::Induction of apoptosis is an approach to suppress carcinogenesis. The effects of a 12-week treatment of female Sprague-Dawley rats with indole-3-carbinol (I3C), beta-naphthoflavone or vehicle (40% ethanol in corn oil), by oral gavages starting 3 weeks after initiation of mammary tumorigenesis with 7,12-dimethylbenz[al...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:High thymidine kinase (TK) activity in cancer cells could counteract adjuvant chemotherapy directed at the inhibition of de novo DNA synthesis. TK is an independent prognostic factor in breast cancer patients receiving adjuvant chemotherapy. MATERIAL AND METHODS:In this paper, we describe the effects of ext...
journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2000-03-01 00:00:00
abstract:AIM:To evaluate the efficacy of prophylactic radiotherapy at intervention sites in patients with malignant pleural mesothelioma (MPM). PATIENTS AND METHODS:From 05/2010 to 12/2014, 53 patients with histologically confirmed MPM were treated in order to prevent interventional site metastases. Irradiation was carried out...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND/AIM:The lipophilic, orally bioavailable camptothecin analogue gimatecan is characterized by improved efficacy over conventional camptothecins on human tumor xenografts. Gimatecan produced excellent outcomes orally with prolonged, low-dose, daily treatments. The aim of this study was to assess the antitumor e...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12917
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Bone defects due to malignant tumor often lead to repeated surgery or amputation. Thus, a major objective in orthopedic surgery is the extracorporeal devitalization of tumor-bearing bone segments following reimplantation. Extracorporeal irradiation or autoclaving are possible methods even though they may cau...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::A universal intracellular factor, the "M phase-promoting factor" (MPF), triggers the G2/M transition of the cell cycle in all organisms. In late G2, it is present as an inactive complex of tyrosine-phosphorylated p34cdc2 and unphosphorylated cyclin Bcdc13. In M phase, its activation as an active MPF displaying histone...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:Chemotherapeutic anticancer properties are thought to derive from apoptosis pathway activation and/or cell division arrest, but animal models have also evidenced anti-angiogenic activity in some agents. PATIENTS AND METHODS:The impact of gemcitabine, irinotecan and oxaliplatin + 5-FU upon the serum markers ...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND/AIM:Non-pegylated liposomal doxorubicin (NPLD) has demonstrated antitumour activity equivalent to conventional doxorubicin and a significantly lower risk of cardiotoxicity. This phase II trial was performed to evaluate the activity and the safety of NPLD and ifosfamide combination in patients with metastatic...
journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2015-01-01 00:00:00
abstract::Combination therapies are used in the clinic to achieve cure, better efficacy and to circumvent resistant disease in patients. Initial assessment of the effect of such combinations, usually of two agents, is frequently performed using in vitro assays. In this review, we give a short summary of the types of analyses th...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.13472
更新日期:2019-07-01 00:00:00
abstract::Iceland has a total population of 300,000 inhabitants. All patients consulting for symptoms of the lower digestive tract during a four-year period (2003-2006) were subjected to a colonoscopic examination; all polyps were endoscopically removed. Out of the total 3,037 colorectal adenomas (CRAs), 308 (10.2%) were tradit...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to identify the DNA methylation status of the mitogen-activated protein kinase (MAPK) signal-inhibiting genes dual-specificity phosphatase 4 (DUSP4) and 6 (DUSP6); and serpin peptidase inhibitor A member 5 (SERPINA5) in thyroid cancer. MATERIALS AND METHODS:Using 76 papillary t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND/AIM:DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS:miRNAs which were up-regulated after de-methylatio...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract::The value of metabolic proton NMR spectroscopic imaging to detect and classify tumours increases with the spatial resolution and the information content of the spectra. Several factors influencing these quantities are discussed. ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Amrubicin is an active agent for the treatment of small-cell lung cancer (SCLC). However, there have been no reports of long-term amrubicin use. PATIENTS AND METHODS:Twelve patients with SCLC who were treated with eight or more cycles of amrubicin chemotherapy were retrospectively reviewed. RESULTS:The med...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::Tumour growth curve experiments play an important role in experimental oncology and established methods exist for their unbiased interpretation. However, many authors till introduce systemic biases to their data by using the so-called "relative tumour size" transformation, inspite of wide awarance about the limitation...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract:BACKGROUND/AIM:The aim of this study is to find synergistic effect using FH535 and sorafenib by targeting the RAS/RAF/MAPK and WNT/β-catenin pathways. MATERIALS AND METHODS:3H-Thymidine incorporation assays were performed to address Huh7 and liver cancer stem cell (LCSC) inhibition using FH535 and sorafenib, alone and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND/AIM:Gemcitabine is a drug commonly used to treat pancreatic cancer but chemoresistance to it is a common clinical issue. KML001 (sodium meta-arsenite) has demonstrated certain antitumor activity. The objective of the study was to evaluate the influence of KML001 on the anticancer activity of gemcitabine agai...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Angiogenesis is critical for tumor growth and inflammation. Shiunko is a Chinese herbal ointment used for the treatment of burns in Japan. Its main ingredient is the root of Lithospermum erythrorhizon, which had been used for treating tumors and inflammation in China since the 5th century. We report here that shikonin...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND:The standard treatment of epithelial ovarian cancer is tumor debulking by surgery, followed by six cycles of chemotherapy consisting of cisplatinum and paclitaxel. However, this therapy protocol is not satisfactory, since about 50% of the treated patients eventually experience recurrence within a few years o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND/AIM:Insulin-like growth factor II mRNA-binding protein 3 (IMP3) plays an important role in the adhesion, invasion, and metastasis of tumor cells. Although emerging evidence suggests that IMP3 promotes tumor progression in several malignancies, the expression of IMP3 and its prognostic implication in adenocar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13683
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Chkl is a checkpoint gene that is activated after DNA damage. It phosphorylates and inactivates Cdc25C at the late G2 phase. The inactivation of Cdc25C and consequently, the inactivation of Cdc2, are required for the G2 arrest induced by DNA damage. METHODS:We treated 184B5 cell line and its E6 transformed ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Nuclear morphometry can be expected to improve the distinction between benign and malignant lesions. PATIENTS AND METHODS:Forty fine-needle aspiration biopsy (FNAB) samples fixed in 50% ethanol were collected at the African Oncology Institute, Sabratha, Libya, during the period 2004-2007. All diagnoses repo...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::The stems of Bougainvillea spectabillis Wild (Nyctaginaceae) have been used in folk medicine for hepatis, and spinasterol and quercetin were isolated and characterized from the plant leaves in this study. These constituents have not been previously isolated from Bougainvillea spectabillis W. Quercetin, the flavonoid, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00