Abstract:
:STX1 is a major neuronal syntaxin protein located at the plasma membrane of the neuronal tissues. Rodent STX1 has two highly similar paralogs, STX1A and STX1B, that are thought to be functionally redundant. Interestingly, some studies have shown that the distribution patterns of STX1A and STX1B at the central and peripheral nervous systems only partially overlapped, implying that there might be differential functions between these paralogs. In the current study, we generated an STX1B knockout (KO) mouse line and studied the impact of STX1B removal in neurons of several brain regions and the neuromuscular junction (NMJ). We found that either complete removal of STX1B or selective removal of it from forebrain excitatory neurons in mice caused premature death. Autaptic hippocampal and striatal cultures derived from STX1B KO mice still maintained efficient neurotransmission compared with neurons from STX1B wild-type and heterozygous mice. Interestingly, examining high-density cerebellar cultures revealed a decrease in the spontaneous GABAergic transmission frequency, which was most likely due to a lower number of neurons in the STX1B KO cultures, suggesting that STX1B is essential for neuronal survival in vitro. Moreover, our study also demonstrated that although STX1B is dispensable for the formation of the mouse NMJ, it is required to maintain the efficiency of neurotransmission at the nerve-muscle synapse.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Wu YJ,Tejero R,Arancillo M,Vardar G,Korotkova T,Kintscher M,Schmitz D,Ponomarenko A,Tabares L,Rosenmund Cdoi
10.1152/jn.00577.2015subject
Has Abstractpub_date
2015-10-01 00:00:00pages
2404-17issue
4eissn
0022-3077issn
1522-1598pii
jn.00577.2015journal_volume
114pub_type
杂志文章abstract::1. We have stimulated responses of stellate cells in the anteroventral cochlear nucleus (AVCN) to single-formant stimuli (SFSs) with the use of recorded auditory-nerve fiber (ANF) responses as inputs. In particular, two important features of temporal discharge patterns, the phase locking to best frequency (BF) tones a...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.73.4.1600
更新日期:1995-04-01 00:00:00
abstract::The concept of hierarchical motor control has been viewed as a means of progressively decreasing the number of variables manipulated by each higher control level. We tested the hypothesis that turning an individual bimanual force-production task into a joint (two-participant) force-production task would lead to positi...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00313.2015
更新日期:2015-06-01 00:00:00
abstract::Monoamines are strong modulators and/or activators of spinal locomotor networks. Thus monoaminergic fibers likely contact neurons involved in generating locomotion. The aim of the present study was to investigate the serotonergic innervation of locomotor-activated neurons within the thoraco-lumbar spinal cord followin...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.91179.2008
更新日期:2009-09-01 00:00:00
abstract::1. In a search for correlated fast rhythms in recurrent laryngeal (RL) expiratory (E) activities, we performed spectral and coherence analysis on bilateral RL nerve discharges in 54 midcollicular decerebrate, paralyzed cats. 2. Inspiratory (I) RL activities showed in almost all cases high-frequency oscillations (HFO, ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.70.5.2192
更新日期:1993-11-01 00:00:00
abstract::Whole cell patch-clamp recordings were used to investigate the contribution of transient, low-threshold calcium currents (I(T)) to firing properties of hamster spinal dorsal horn neurons. I(T) was widely, though not uniformly, expressed by cells in Rexed's laminae I-IV and correlated with the pattern of action potenti...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01083.2010
更新日期:2011-11-01 00:00:00
abstract::The same set of stimuli and analytic methods that was used to study the dynamics of horizontal cells () was applied to a study of the response dynamics and signal processing in amacrine cells in the retina of the kissing gourami, Helostoma rudolfi. The retina contains two major classes of amacrine cells that could be ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.78.4.2018
更新日期:1997-10-01 00:00:00
abstract::1. The sensorimotor synapse of Aplysia expresses various shortlasting changes in synaptic efficacy including homosynaptic depression (HSD) and heterosynaptic facilitation by serotonin (5-HT) either at nondepressed sensory neuron (SN) synaptic connections or at SN synaptic connections first depressed by HSD. We examine...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.4.2250
更新日期:1996-10-01 00:00:00
abstract::1. The effects of inhibitory amino acid transmitters on horizontal cells in the superfused amphibian retina were studied by the use of conventional intracellular recording techniques. 2. Gamma-aminobutyric acid (GABA) caused a calcium-independent depolarization of horizontal cells in mud puppy and tiger salamander. Th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.65.3.680
更新日期:1991-03-01 00:00:00
abstract::Odor identification (OI) tests are increasingly used clinically as biomarkers for Alzheimer's disease and schizophrenia. The aim of this study was to directly compare the neuronal correlates to identified odors vs. nonidentified odors. Seventeen females with normal olfactory function underwent a functional magnetic re...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01036.2010
更新日期:2012-07-01 00:00:00
abstract::It is controversial whether simple-spike activity of cerebellar Purkinje cells during arm movements encodes movement kinematics like velocity or dynamics like muscle activities. To examine this issue, we trained monkeys to flex or extend the elbow by 45 degrees in 400 ms under resistive and assistive force fields but ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00206.2006
更新日期:2007-02-01 00:00:00
abstract::Trimming all but two whiskers in adult rats produces a predictable change in cortical cell-evoked responses characterized by increased responsiveness to the two intact whiskers and decreased responsiveness to the trimmed whiskers. This type of synaptic plasticity in rat somatic sensory cortex, called "whisker pairing ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.6.3216
更新日期:1998-06-01 00:00:00
abstract::The holoplanktonic pteropod mollusk Clione limacina exhibits an active escape behavior that is characterized by fast swimming away from the source of potentially harmful stimuli. The initial phase of escape behavior is a startle response that is controlled by pedal motoneurons whose activity is independent of the norm...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.1.281
更新日期:1997-01-01 00:00:00
abstract::The centre médian-parafascicular (CM-Pf) complex is located at the posterior intralaminar nuclei of the thalamus and forms part of the nonspecific thalamocortical projection system and the internal circuit of the basal ganglia. However, the functional roles of this complex remain to be fully elucidated. Here we have e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.87.6.3090
更新日期:2002-06-01 00:00:00
abstract::Substance P (SP) hyperpolarizes approximately 80% of ferret vagal sensory neurons (nodose ganglion neurons) via NK-1 receptor-mediated activation of a potassium current (IK). A depolarizing current activated by membrane hyperpolarization could minimize the SP-induced hyperpolarization. Such a current exists in 65% of ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.2.769
更新日期:1998-02-01 00:00:00
abstract::Few studies have analyzed activity-induced changes in EMG activity in individual human motor units. We studied the changes in human thenar motor unit EMG that accompany the potentiation of twitch force and fatigue of tetanic force. Single motor unit EMG and force were recorded in healthy subjects in response to select...
journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章
doi:10.1152/jn.00924.2005
更新日期:2006-03-01 00:00:00
abstract::Electrical synapses play significant roles in neural processing in invertebrate and vertebrate nervous systems. The view of electrical synapses as plain bidirectional intercellular channels represents a partial picture because rectifying electrical synapses expand the complexity in the communication capabilities of ne...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00973.2006
更新日期:2007-02-01 00:00:00
abstract::Serotonin (5-hydroxytryptamine, 5-HT) inhibits the induction of long-term synaptic plasticity in layer 2/3 of the visual cortex at the end of its critical period in rats. However, the cellular and molecular mechanisms remain unclear. Since inhibitory influence is crucial in the induction of synaptic plasticity, the ef...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00535.2011
更新日期:2012-01-01 00:00:00
abstract::The responses of cortical neurons to repeated presentation of a stimulus are highly variable, yet correlated. These "noise correlations" reflect a low-dimensional structure of population dynamics. Here, we examine noise correlations in 22,705 pairs of neurons in primary visual cortex (V1) of anesthetized cats, during ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00094.2015
更新日期:2015-08-01 00:00:00
abstract::The strength-duration relationship for extracellular stimulation is often assumed to be similar to the classical intracellular stimulation model, with a slope asymptotically approaching 1/τ at pulse durations shorter than chronaxy. We modeled extracellular neural stimulation numerically and analytically for several ce...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00343.2010
更新日期:2010-10-01 00:00:00
abstract::People perceive better in cardinal directions compared with oblique ones. This directional effect, called oblique effect, has been documented in perception studies for a long time. However, typical motor studies do not differentiate learning in different directions. In this study we identify a significant directional ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00787.2017
更新日期:2018-07-01 00:00:00
abstract::N-arachidonoyldopamine (NADA) is an endogenous molecule found in the nervous system that is capable of acting as a vanilloid agonist via the TRPV1 receptor and as a cannabinoid agonist via the CB1 receptor. Using anesthetized rats, we investigated the neural correlates of behavioral thermal hyperalgesia produced by NA...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00395.2005
更新日期:2006-02-01 00:00:00
abstract::Neurons in the visual cortex respond selectively to stimulus orientation and spatial frequency. Changes in response amplitudes of these neurons could be the neurophysiological basis of orientation and spatial frequency discrimination. We have estimated the minimum differences in stimulus orientation and spatial freque...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.3.755
更新日期:1987-03-01 00:00:00
abstract::The possibility of using randomly generated stimulus intervals (with a Poisson distribution) to identify the type(s) of intrafusal fiber activated by the stimulation of single static gamma axons was tested in Peroneus tertius muscle spindles of anesthetized cats. Three patterns of random stimulation with different val...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.81.6.2823
更新日期:1999-06-01 00:00:00
abstract::1. Bicuculline-induced epileptiform bursts in slices of neocortical tissue resected from children (3 mo to 14 yr) undergoing neurosurgical treatment for intractable epilepsy were studied with conventional intracellular recording techniques. The purposes of this study were to characterize the bursts evoked in immature ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.70.5.1759
更新日期:1993-11-01 00:00:00
abstract::1. Discharges of neurons in the peripheral auditory system contain information about the temporal features of acoustic stimuli. Phase-locking of neurons in the anteroventral cochlear nucleus (AVCN) is usually reported to be less robust than in auditory nerve (AN) fibers, which provide their major input. In a companion...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.3.1037
更新日期:1994-03-01 00:00:00
abstract::PSD-95-like, disc-large (DLG) family membrane-associated guanylate kinase proteins (PSD/DLG-MAGUKs) are essential for regulating synaptic AMPA receptor (AMPAR) function and activity-dependent trafficking of AMPARs. Using a molecular replacement strategy to replace endogenous PSD-95 with SAP97β, we show that the protot...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00262.2013
更新日期:2014-02-01 00:00:00
abstract::This paper compares the activity of hindlimb motor units from muscles mainly composed of fast-twitch muscle fibers (medial and lateral gastrocnemius: MG/LG, tibialis anterior: TA) to motor units from a muscle mainly composed of slow-twitch muscle fibers (soleus: SOL) during unrestrained walking in the conscious rat. S...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.4.2002
更新日期:2000-04-01 00:00:00
abstract::When the head is free to move, electrical stimulation in the frontal eye field (FEF) evokes eye and head movements. However, it is unclear whether FEF stimulation-evoked head movements contribute to shifting the line of sight, like visually guided coordinated eye-head gaze shifts. Here we investigated this issue by sy...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01320.2005
更新日期:2006-06-01 00:00:00
abstract::Combined transcranial magnetic stimulation and electroencephalography (TMS-EEG) enables noninvasive neurophysiological investigation of the human cortex. A TMS paradigm of short-latency afferent inhibition (SAI) is characterized by attenuation of the motor-evoked potential (MEP) and modulation of N100 of the TMS-evoke...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00260.2016
更新日期:2016-09-01 00:00:00
abstract::1. The response properties of 39 periodontal ligament mechanoreceptors (PDLMs) and 12 intradental mechanoreceptors (IMs) related to the intact mandibular canine tooth were isolated by extracellular recording methods from the ipsilateral trigeminal semilunar ganglion. 2. The stimulus threshold and response magnitude of...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.69.5.1567
更新日期:1993-05-01 00:00:00