Abstract:
:All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transporters from the ABC superfamily, found in most species - from bacteria to humans. An important example of such a transporter is the breast cancer resistance protein (BCRP/ABCG2), a typical integral membrane protein. It plays a key role in the absorption, distribution and elimination of a wide variety of xenobiotics, including drugs used in chemotherapy, and is involved in multidrug resistance. It also protects against phototoxic chlorophyll derivatives of dietary origin. BCRP is a hemitransporter which consists of one transmembrane domain, made of six alpha-helices forming a characteristic pore structure, and one ATP-binding domain, which provides the energy from ATP hydrolysis, required for active transport of the substrates. The isolation of BCRP is still not an easy task, because its insolubility in water and the presence of membrane rafts pose serious methodological and technical challenges during the purification. The aim of this study was to optimize the methods for detection and isolation of BCRP-enriched fractions obtained from animal tissue samples. In this report we describe an optimization of isolation of a BCRP-enriched membrane fraction, which is suitable for further protein quantitative and qualitative analysis using the molecular biology tools.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Szczygieł M,Markiewicz M,Szafraniec M,Zuziak R,Urbańska K,Fiedor Ldoi
10.18388/abp.2017_2299subject
Has Abstractpub_date
2017-01-01 00:00:00pages
437-443issue
3eissn
0001-527Xissn
1734-154Xpii
2299journal_volume
64pub_type
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
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doi:
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pub_type: 杂志文章
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
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更新日期:2006-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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