Plasma levels of VEGF-A, VEGF B, and VEGFR-1 and applicability of these parameters as tumor markers in diagnosis of breast cancer.

Abstract:

:The VEGF family members are important factors in promoting angiogenesis and lymphangiogenesis in malignant processes. The aim of this study was to investigate plasma concentrations of VEGF-A, VEGF-B and their soluble VEGFR-1 receptor and their diagnostic utility and potency as compared to CA 15-3 in breast cancer patients and in relation to the control group. The study included 120 breast cancer patients and 60 control patients. Plasma levels of tested parameters were determined with ELISA and CA 15-3 levels were determined with CMIA. Concentrations of all tested parameters in breast cancer patients showed statistically significant difference when compared to the control groups (benign breast tumor patients and/or healthy women). VEGF-B showed the highest values of sensitivity (Sn) and predictive value of a negative test result (NPV) in total BC group (90% and 66.7%, respectively) and, more importantly, in stages I-II of BC (SE: 86.8%; 92.7%, NPV: 82.8%; 88.9%, respectively). Among all parameters tested, VEGF-A showed the highest specificity (Sf) (76.7%) and predictive value of a positive test result (PPV) (84.8%), yet they were lower than for CA 15-3. VEGF-A was also the best parameter that had statistically significant Area Under Curve (AUC) in stages I (0.678) and II (0.768). In the whole group of BC patients all parameters tested showed statistically significant AUC, but the maximum range was obtained for the combination of VEGF-A and CA 15-3 (0.817). The combined analysis of the studied parameters and CA 15-3 resulted in an increase in sensitivity and AUC values, which provides hope for developing a new panel of biomarkers that may be used in BC diagnosis in the future.

journal_name

Acta Biochim Pol

journal_title

Acta biochimica Polonica

authors

Zajkowska M,Lubowicka E,Malinowski P,Szmitkowski M,Ławicki S

doi

10.18388/abp.2018_2713

subject

Has Abstract

pub_date

2018-12-13 00:00:00

pages

621-628

issue

4

eissn

0001-527X

issn

1734-154X

pii

2713

journal_volume

65

pub_type

杂志文章
  • Comparative studies on fish and bird insulin receptors.

    abstract::The comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). The Scatchard plots indicated the presence of two pools of binding sites both in fish and duck. These pools show inter-species differences in binding ability and the number o...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Nowak KW,Maćkowiak P,Nogowski L

    更新日期:1993-01-01 00:00:00

  • Halogen bonds involved in binding of halogenated ligands by protein kinases.

    abstract::Analysis of 664 known structures of protein kinase complexes with halogenated ligands revealed 424 short contacts between a halogen atom and a potential protein X-bond acceptor, the topology and geometry of which were analyzed according to the type of a halogen atom (X = Cl, Br, I) and a putative protein X-bond accept...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章,评审

    doi:10.18388/abp.2015_1106

    authors: Poznański J,Winiewska M,Czapinska H,Poznańska A,Shugar D

    更新日期:2016-01-01 00:00:00

  • Plasma membrane homing of tissue nonspecific alkaline phosphatase under the influence of 3-hydrogenkwadaphnin, an antiproliferative agent from Dendrostellera lessertii.

    abstract::Several mammalian enzymes are anchored to the outer surface of the plasma membrane by a covalently attached glycosylphosphatidylinositol (GPI) structure. These include acetylcholinesterase, alkaline phosphatase (AP) and 5'-nucleotidase among other enzymes. Recently, it has been reported that these membrane enzymes can...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Sadeghirizi A,Yazdanparast R

    更新日期:2007-01-01 00:00:00

  • Recent progress in oligonucleotide synthesis.

    abstract::New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fulfilled in ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章,评审

    doi:

    authors: Pfleiderer W,Matysiak S,Bergmann F,Schnell R

    更新日期:1996-01-01 00:00:00

  • Cytostatic and cytotoxic effects of (E)-2'-deoxy-2'-(fluoromethylene)-cytidine on a solid tumor and a leukemia cell line.

    abstract::(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Grie P,Koronkiewicz M,Skierski JS

    更新日期:2000-01-01 00:00:00

  • Searching for anti-glioma activity. Ribonucleoside analogues with modifications in nucleobase and sugar moieties.

    abstract::Several ribonucleoside analogues with modifications in the nucleobase and sugar moiety have been screened for anti-glioma activity in the T98G glioma cell line using cervical (HeLa) cell line as reference human malignant cells, and lung fibroblast (MCR-5) cell line as non-cancerous reference cells. Among the investiga...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:10.18388/abp.2016_1338

    authors: Framski G,Wawrzyniak D,Jahnz-Wechmann Z,Szymanska-Michalak A,Kraszewski A,Barciszewski J,Boryski J,Stawinski J

    更新日期:2016-01-01 00:00:00

  • The mechanism of azide activation of polyphenol oxidase II from tobacco.

    abstract::So far, azide has been consistently reported to act as an inhibitor of metal enzymes, especially copper proteins. The present work shows that azide can also act as an activator of polyphenol oxidase II (PPO II) from tobacco leaves. From the square-wave voltammetry of native PPO II, peroxide-PPO II complex and azide-PP...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:0249041029

    authors: Shi C,Liu Q,Dai Y,Xie Y,Xu X

    更新日期:2002-01-01 00:00:00

  • Photosynthesis in detached rye leaves at normal and low oxygen concentration. II. Incorporation of 14CO2 into amino acids.

    abstract::The pools of Arg, Gly, His, Ile, Ser, Glu, Leu, Val and Asp were lower during photosynthesis at 1% O2 concentration. At the same time specific radioactivity of a number of amino acids--following 14CO2 incorporation--was different than that at atmospheric O2 concentration. This is probably due to the inhibition of the ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Niziołek S,Bielawski W,Nalborczyk E

    更新日期:1982-01-01 00:00:00

  • Interaction of glycophorin A with lectins as measured by surface plasmon resonance (SPR).

    abstract::Glycophorin A (GPA), the major sialoglycoprotein of the human erythrocyte membrane, was isolated from erythrocytes of healthy individuals of blood groups A, B and O using phenol-water extraction of erythrocyte membranes. Interaction of individual GPA samples with three lectins (Psathyrella velutina lectin, PVL; Tritic...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Krotkiewska B,Pasek M,Krotkiewski H

    更新日期:2002-01-01 00:00:00

  • Evaluation of antioxidant, antimutagenic, and lipid peroxidation inhibitory activities of selected fractions of Holarrhena floribunda (G. Don) leaves.

    abstract::Exposure to environmental pollutants often leads to an upsurge in the production of reactive oxygen species (ROS). ROS oxidize cellular fatty acids to produce lipid peroxyl radicals, subsequently transformed into lipid peroxides, which decrease membrane fluidity and increase the activity of various enzymes implicated ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Badmus JA,Odunola OA,Yekeen TA,Gbadegesin AM,Fatoki JO,Godo MO,Oyebanjo KS,Hiss DC

    更新日期:2013-01-01 00:00:00

  • Purification of geranylgeranyl diphosphate synthase from bovine brain.

    abstract::Geranylgeranyl diphosphate (GGPP) synthase was purified to homogeneity from bovine brain in a one-step affinity column procedure. For the construction of the affinity column, a farnesyl diphosphate (FPP) analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the ma...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Sagami H,Morita Y,Korenaga T,Ogura K

    更新日期:1994-01-01 00:00:00

  • Lymphocyte labile iron pool, plasma iron, transferrin saturation and ferritin levels in colon cancer patients.

    abstract::Patients with colorectal carcinoma showed statistically significant lower values of transferrin saturation, total iron binding capacity and serum iron level as compared with control group, while the level of ferritin and the size of labile iron pool in carcinoma patients were higher, although this difference was not s...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Gackowski D,Kruszewsk M,Banaszkiewicz Z,Jawien A,Olinski R

    更新日期:2002-01-01 00:00:00

  • Beta-carotene enhances the recovery of lymphocytes from oxidative DNA damage.

    abstract::Epidemiological studies have revealed a strong correlation between high intake of fruit and vegetables and low incidence of certain cancers. Micronutrients present in these foods are thought to decrease free radical attack on DNA and hence protect against mutations that cause cancer, but the fine details of the causal...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Fillion L,Collins A,Southon S

    更新日期:1998-01-01 00:00:00

  • Effect of thyroid hormone on the myosin heavy chain isoforms in slow and fast muscles of the rat.

    abstract::The myosin heavy chain (MHC) was studied by biochemical methods in the slow-twitch (soleus) and two fast-twitch leg muscles of the triiodothyronine treated (hyperthyroid), thyroidectomized (hypothyroid) and euthyroid (control) rats. The changes in the contents of individual MHC isoforms(MHC-1, MHC-2A, MHC-2B and MHC-2...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Jakubiec-Puka A,Ciechomska I,Mackiewicz U,Langford J,Chomontowska H

    更新日期:1999-01-01 00:00:00

  • A levels of endogenous gonadal hormones and their relationship with selected coronary artery disease risk factors among young women post myocardial infarction.

    abstract::In recent decades a significant raise in the incidence of myocardial infarction among young women has been recorded. It is presumed that, apart from the classical risk factors, other reasons exist for premature atherosclerosis in young women, related to the homeostasis of gonadal hormones. The aim of the study was to ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Ablewska U,Jankowski K,Rzewuska E,Liszewska-Pfejfer D,Hryniewiecki T

    更新日期:2011-01-01 00:00:00

  • Elimination of goat haemoglobin and its complexes with goat haptoglobin from goat and rat circulation.

    abstract::The rate of elimination from rat and goat circulation of 125I-labelled goat haemoglobin and its complexes with human and goat haptoglobin in goat circulation, was studied. The half-life time t1/2 of haemoglobin in rat circulation was 1.5h, whereas in goat circulation -- 4 h, and was 10 times shorter than t1/2 for its ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Osada J,Nowacki W

    更新日期:1989-01-01 00:00:00

  • Signalling: basics and evolution.

    abstract::Signalling concerns the transfer of information from one body, a source, to another, a receiver in order to stimulate activity. The problem arises with the word information. It is defined as what is transferred in a sequence of things, say between people, e.g. words or signs. The idea of signalling between people is t...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章,评审

    doi:035001281

    authors: Williams RJ

    更新日期:2004-01-01 00:00:00

  • Reactions of Pseudomonas aeruginosa pyocyanin with reduced glutathione.

    abstract::Pseudomonas aeruginosa is the most common cause of chronic and recurrent lung infections in patients with cystic fibrosis (CF) whose sputa contain copious quantities of P. aeruginosa toxin, pyocyanin. Pyocyanin triggers tissue damage mainly by its redox cycling and induction of reactive oxygen species (ROS). The react...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Cheluvappa R,Shimmon R,Dawson M,Hilmer SN,Le Couteur DG

    更新日期:2008-01-01 00:00:00

  • Why a "benign" mutation kills enzyme activity. Structure-based analysis of the A176V mutant of Saccharomyces cerevisiae L-asparaginase I.

    abstract::A conservative and apparently harmless A176V mutation in intracellular S. cerevisiae L-asparaginase (ScerAI) completely abolishes the enzyme activity. Sequence and structural comparisons with type II bacterial L-asparaginases show that the mutated residue is in a very conservative region and plays a vital role in the ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章,评审

    doi:

    authors: Bonthron DT,Jaskólski M

    更新日期:1997-01-01 00:00:00

  • The levels of glycosphingolipids, ceramides, sialic acid and glycogen are changed in plasma membranes of rat platelets harvested during recovery from immune-mediated thrombocytopenia.

    abstract::Plasma membranes of rat platelets produced at normal platelet counts and during early recovery from immune-mediated thrombocytopenia were examined for the contents of carbohydrates, lipids and glycosphingolipids. Glucosylceramide, two monosialo-gangliosides and one disialo-ganglioside were found to be the major glycos...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Zdebska E,Soszyńska B,Dobrowolski Z,Kościelak J

    更新日期:1996-01-01 00:00:00

  • Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods.

    abstract::The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:10.18388/abp.2017_1571

    authors: Nawrot U,Sulik-Tyszka B,Kurzyk E,Mroczyńska M,Włodarczyk K,Wróblewska M,Basak GW,Brillowska-Dąbrowska A

    更新日期:2017-01-01 00:00:00

  • Metabolism of exogenous polyisoprenoids by animal cells cultured in vitro.

    abstract::The content and type of dolichols in chicken embryo fibroblasts was estimated and the presence of enzymic activities of various dolichyl phosphate sugar transferases was documented. Chicken embryo fibroblasts effectively take up various polyprenoids from the culture medium and catalyse both formation of polyprenyl fat...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Krajewska-Rychlik I

    更新日期:1985-01-01 00:00:00

  • A freeze-thaw method for disintegration of Escherichia coli cells producing T7 lysozyme used in pBAD expression systems.

    abstract::The pLysN plasmid containing the T7 lysozyme gene under control of the lac promoter was constructed to facilitate cell disintegration after expression of recombinant proteins in arabinose-induced expression systems. The usefulness of this plasmid was tested in Escherichia coli TOP10 and E. coli LMG194 cells carrying p...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Wanarska M,Hildebrandt P,Kur J

    更新日期:2007-01-01 00:00:00

  • In vitro inhibition of topoisomerase IIα by reduced glutathione.

    abstract::In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive sub...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Delwar ZM,Vita MF,Siden Å,Cruz M,Yakisich JS

    更新日期:2011-01-01 00:00:00

  • Interaction of metal ions with nucleic acids. Interaction of copper(II) with inosine and its derivatives.

    abstract::1. The interaction of copper(II) with inosine, 2'-deoxyinosine, 1-methylinosine, 7-deazainosine, 6-methoxypurine riboside and IMP was examined. 2. Copper binds with the purine base of the nucleosides, and in IMP also with the phosphate group. Non interaction with ribose hydroxyl groups was observed. 3. In non-aqueous ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Maskos K

    更新日期:1978-01-01 00:00:00

  • The access of metabolites into yeast mitochondria in the presence and absence of the voltage dependent anion selective channel (YVDAC1).

    abstract::Since yeast Saccharomyces cerevisiae mutants depleted of the voltage dependent anion selective channel (YVDAC1) are still able to grow on a non-fermentable carbon source, a functional transport system in the outer mitochondrial membrane must exist to support the access of metabolites into mitochondria. It was assumed ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Kmita H,Stobienia O,Michejda J

    更新日期:1999-01-01 00:00:00

  • Eriochrome black T as a dye for agarose gel electrophoresis.

    abstract::We found that it is possible to use eriochrome black T as a dye for agarose gel electrophoresis of DNA. The presence of the dye does not change the migration rate of DNA and does not influence the electrophoretical picture. ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Obuchowski M,Wegrzyn G

    更新日期:1991-01-01 00:00:00

  • Two Gln187 mutants of human soluble APRIL inhibit proliferation of lung carcinoma A549 cells.

    abstract::Soluble APRIL (sAPRIL), the active form of a proliferation-inducing ligand (APRIL), is implicated in the proliferation of tumor cells. Suppressing APRIL function has been considered as a potential strategy for the therapy of APRIL-associated tumors. In the present study, we generated human sAPRIL and its two mutants, ...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Dai S,Zheng Y,Chen B,Gao M,Zhang Y,Zhang L,Gong W,He F

    更新日期:2009-01-01 00:00:00

  • Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.

    abstract::Human mitochondrial polynucleotide phosphorylase (hPNPase) is an exoribonuclease localized in mitochondria. The exact physiological function of this enzyme is unknown. Recent studies have revealed the existence of a relationship between induction of hPNPase mRNA and both cellular senescence and growth arrest of melano...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Gewartowski K,Tomecki R,Muchowski L,Dmochow Ska A,Dzwonek A,Malecki M,Skurzak H,Ostrowski J,Stepien PP

    更新日期:2006-01-01 00:00:00

  • On tautomerism of the cytosine molecule.

    abstract::Tautomerism of the cytosine molecule is discussed in connection with recent experimental matrix-isolation infrared spectroscopic measurements and recent ab initio calculations of relative stabilities of tautomers and of IR spectra for different tautomeric forms of the compound. Experimental IR spectra in the N-H and O...

    journal_title:Acta biochimica Polonica

    pub_type: 杂志文章

    doi:

    authors: Kwiatkowski JS,Person WB,Szczepaniak K,Szczesniak M

    更新日期:1987-01-01 00:00:00