Hematological and antifungal properties of temporin A and a cecropin A-temporin A hybrid.

abstract：:The use of nucleotides and their analogs in the pharmacological studies of nucleotide receptors (P2 class) should be preceded by detailed studies on their degradation connected with ecto-enzymes of a given cell type. In the present studies we have analyzed stability of some phosphorothioate and phosphonate analogs of ...

journal_title：Acta biochimica Polonica

authors： Gendaszewska-Darmach E,Maszewska M,Zakłos M,Koziołkiewicz M

更新日期：2003-01-01 00:00:00

abstract：:We examined the molecular basis of factor IX deficiency in 53 unrelated Polish patients with hemophilia B. Heteroduplex analysis and direct sequencing of polymerase chain reaction (PCR) products were applied to identify the gene defect. Forty-three different point mutations were detected in the factor IX gene of 47 pa...

journal_title：Acta biochimica Polonica

authors： Wulff K,Bykowska K,Lopaciuk S,Herrmann FH

更新日期：1999-01-01 00:00:00

abstract：:An increasing body of evidence suggests that glycolipid domains are present on the plasma membrane surface of mammalian cells and play a key role in signal transduction. We have investigated the modulation of glycolipid-protein interaction consequent to a specific event occurring at the plasma membrane. For this purpo...

journal_title：Acta biochimica Polonica

authors： Masserini M,Pitto M,Ferraretto A,Brunne J,Palestini P

更新日期：1998-01-01 00:00:00

abstract：:Bacteriophage lambda is not able to lysogenise the Escherichia coli rpoA341 mutant. This mutation causes a single amino acid substitution Lys271Glu in the C-terminal domain of the RNA polymerase alpha subunit (alphaCTD). Our previous studies indicated that the impaired lysogenisation of the rpoA341 host is due to a de...

journal_title：Acta biochimica Polonica

authors： Gabig M,Obuchowski M,Ciesielska A,Latała B,Wegrzyn A,Thomas MS,Wegrzyn G

更新日期：1998-01-01 00:00:00

abstract：:Aberrant expression of sialic acids or altered linkage types is closely associated with malignant phenotype and metastatic potential, and can have prognostic significance in human cancer. The present study was undertaken to evaluate whether expression of sialylated derivatives on melanoma cell surface is associated wi...

journal_title：Acta biochimica Polonica

authors： Kolasińska E,Przybyło M,Janik M,Lityńska A

更新日期：2016-01-01 00:00:00

abstract：:We investigated the effect of the cyclic AMP-protein kinase A (PKA) signalling pathway on renal Na(+),K(+)-ATPase and ouabain-sensitive H(+),K(+)-ATPase. Male Wistar rats were anaesthetized and catheter was inserted through the femoral artery into the abdominal aorta proximally to the renal arteries for infusion of th...

journal_title：Acta biochimica Polonica

authors： Bełtowski J,Marciniak A,Wójcicka G,Górny D

更新日期：2003-01-01 00:00:00

abstract：:The photochemical characteristics of mesophyll and bundle sheath chloroplasts isolated from the leaves of C4 species were investigated in Zea mays (NADP-ME type), Panicum miliaceum (NAD-ME type) and Panicum maximum (PEP-CK type) plants. The aim of this work was to gain information about selected photochemical properti...

journal_title：Acta biochimica Polonica

authors： Romanowska E,Drozak A

更新日期：2006-01-01 00:00:00

abstract：:Secondary structure of alpha (light) and beta (heavy) chains of human haptoglobin was proposed. The Chou-Fasman predictive method was used in conjunction with the hydrophobic character and hydropathy scale value assigned to each amino acid residue in the primary sequence of the protein. Moreover, by means of hydrophil...

journal_title：Acta biochimica Polonica

authors： Dobryszycka W,Kubisa W,Kubicz Z

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND:In this work we studied the relationship between the enhanced expression of DR5 receptor and the effect of combination of TRAIL and ionizing radiation on cell cycle arrest and apoptosis induction in human leukemia cell line HL-60. MATERIAL AND METHODS:DR5, APO2.7 and cell cycle were analyzed by flow cytomet...

journal_title：Acta biochimica Polonica

authors： Rezácová M,Vávrová J,Vokurková D,Tichý A,Knízek J,Psutka J

更新日期：2005-01-01 00:00:00

abstract：:The content and type of dolichols in chicken embryo fibroblasts was estimated and the presence of enzymic activities of various dolichyl phosphate sugar transferases was documented. Chicken embryo fibroblasts effectively take up various polyprenoids from the culture medium and catalyse both formation of polyprenyl fat...

journal_title：Acta biochimica Polonica

authors： Krajewska-Rychlik I

更新日期：1985-01-01 00:00:00

abstract：:A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity ...

journal_title：Acta biochimica Polonica

authors： Nałecz KA,Zambrowicz BE

更新日期：1990-01-01 00:00:00

abstract：:Cultivated carrot and its wild ancestor co-occur in most temperate regions of the world and can easily hybridize. The genetic basis of the process of domestication in carrot is not well understood. Recent results of an investigation on genetic diversity structure of cultivated and wild carrot and signatures for domest...

journal_title：Acta biochimica Polonica

authors： Macko-Podgórni A,Iorizzo M,Smółka K,Simon PW,Grzebelus D

更新日期：2014-01-01 00:00:00

abstract：:Initiation of replication of lambda DNA requires assembly of the proper nucleoprotein complex consisting of the lambda origin of replication-lambda O-lambda P-dnaB proteins. The dnaJ, dnaK and grpE heat shock proteins destabilize the lambda P-dnaB interaction in this complex permitting dnaB helicase to unwind lambda D...

journal_title：Acta biochimica Polonica

authors： Osipiuk J,Zylicz M

更新日期：1991-01-01 00:00:00

abstract：:Several studies employed the genome-wide association (GWA) analysis of single-nucleotide polymorphisms (SNPs) to identify susceptibility regions in colorectal cancer (CRC). However, the functional studies exploring the role of associating SNPs with cancer biology are limited. Herein, using chromatin immunoprecipitatio...

journal_title：Acta biochimica Polonica

authors： Statkiewicz M,Maryan N,Kulecka M,Kuklinska U,Ostrowski J,Mikula M

更新日期：2019-09-17 00:00:00

abstract：:A protease with a molecular mass of 30 kDa and the N-terminal sequence of GLQTNAPWGLARSS, was isolated from fresh fruiting bodies of the wild edible mushroom Termitomyces albuminosus. The purification protocol included ion exchange chromatography on DEAE-cellulose, Q-Sepharose, SP-Sepharose and FPLC-gel filtration on ...

journal_title：Acta biochimica Polonica

authors： Zheng S,Wang H,Zhang G

更新日期：2011-01-01 00:00:00

abstract：:Soluble APRIL (sAPRIL), the active form of a proliferation-inducing ligand (APRIL), is implicated in the proliferation of tumor cells. Suppressing APRIL function has been considered as a potential strategy for the therapy of APRIL-associated tumors. In the present study, we generated human sAPRIL and its two mutants, ...

journal_title：Acta biochimica Polonica

authors： Dai S,Zheng Y,Chen B,Gao M,Zhang Y,Zhang L,Gong W,He F

更新日期：2009-01-01 00:00:00

abstract：:The dairy propionibacteria, which are traditionally used for the production of Swiss cheeses, are able to synthesize valuable biomolecules, e.g. B group vitamins, propionic acid, and trehalose with unique chemical and physical properties. Both, dairy propionibacteria cells and trehalose, have found many applications a...

journal_title：Acta biochimica Polonica

authors： Pawlicka-Kaczorowska J,Czaczyk K

更新日期：2017-01-01 00:00:00

abstract：:Eight polymorphic restriction enzyme sites at phenylalanine hydroxylase locus from the parental chromosomes in Polish families with phenylketonuria were analyzed. Among 28 chromosomes studied, we identified haplotypes found within the Danish population. Haplotype 2 has been found in 25% of affected alleles. One of the...

journal_title：Acta biochimica Polonica

authors： Jaruzelska J,Borski K,Riess O,Blin N,Słomski R

更新日期：1989-01-01 00:00:00

abstract：:Leukemia is a particular type of cancer characterized by the failure of cell death or disability in differentiation of hematopoietic cells. Chronic myelogenus leukemia (CML) is the most studied kind of this cancer. In this study, anti-cancer effect of dihydro-pyranochromenes derivatives were investigated in the human ...

journal_title：Acta biochimica Polonica

authors： Rahimi R,Mahdavi M,Pejman S,Zare P,Balalaei S

更新日期：2015-01-01 00:00:00

abstract：:Dansylated analogues of the potent and selective micro opioid peptide agonist [Dmt(1)]DALDA (H-Dmt-D-Arg-Phe-Lys-NH(2); Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of N(beta)-dansyl-alpha,beta-diaminopropionic acid or N(epsilon)-dansyllysine for Lys(4), or by attachment of a dansyl group to the ...

journal_title：Acta biochimica Polonica

authors： Berezowska I,Lemieux C,Chung NN,Zelent B,Schiller PW

更新日期：2004-01-01 00:00:00

abstract：:The lifetime distribution calculations were applied to study the influence of configuration of amino-acid residues in positions 2 and 3 on changes in conformation of the peptide chain of cyclic analogues of enkephalins containing a fluorescence energy donor and acceptor in different solvents. In all the solvents studi...

journal_title：Acta biochimica Polonica

authors： Malicka J,Groth M,Czaplewski C,Liwo A,Wiczk W

更新日期：1999-01-01 00:00:00

abstract：:The immunodiffusion cross-reactivity and competitive inhibition ELISA assays were used for immunological differentiation of latent form, cleaved form and guanidinium hydrochloride (GuHCl) induced polymer of human alpha 1-proteinase inhibitor (alpha 1-PI). Under the conditions studied, the differences between latent fo...

journal_title：Acta biochimica Polonica

authors： Wawrzos I,Kitagawa Y,Kołoczek H

更新日期：1996-01-01 00:00:00

abstract：:1. The everted-sac technique has been used to study the time-dependent effect of low-calcium diet on calcium active transport along rat small intestine. 2. In animals maintained on standard diet active translocation of calcium was limited to proximal 10 cm of the intestine. 3. In response to calcium restriction, calci...

journal_title：Acta biochimica Polonica

authors： Michalska L,Wróbel J,Szczepańska-Konkel M

更新日期：1976-01-01 00:00:00

abstract：BACKGROUND:Recent studies showed that sushi repeat containing protein X linked 2 (SRPX2) could participate in the development of various malignant tumors. However, its role in non-small cell lung cancer (NSCLC) was unknown. The aim of the study was to prospectively investigate the role of SRPX2 in NSCLC cell proliferat...

journal_title：Acta biochimica Polonica

authors： Li X,Liu J,Sun H,Zou Y,Chen J,Chen Y,Chen C,Wu X

更新日期：2020-06-18 00:00:00

abstract：:Glycosylation is the most common chemical process of protein modification and occurs in every living cell. Disturbances of this process may be either congenital or acquired. Congenital disorders of glycosylation (CDG) are a rapidly growing disease family, with about 50 disorders reported since its first clinical descr...

journal_title：Acta biochimica Polonica

authors： Cylwik B,Naklicki M,Chrostek L,Gruszewska E

更新日期：2013-01-01 00:00:00

abstract：:In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive sub...

journal_title：Acta biochimica Polonica

authors： Delwar ZM,Vita MF,Siden Å,Cruz M,Yakisich JS

更新日期：2011-01-01 00:00:00

abstract：:Nano- and microemulsions containing as the oil phase caprylic/capric propylene glycol diesters (Crodamol PC) were investigated as potential vehicle for controlled release of geranic acid. The influence of emulsifiers and co-surfactants on stability of the emulsions was investigated. Different kind of polysorbates (eth...

journal_title：Acta biochimica Polonica

authors： Jaworska M,Sikora E,Ogonowski J,Konieczna M

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Tunneled catheters are becoming increasingly used as a permanent dialysis access. Easy way of insertion and good long-term patency make them competitive to fistulas in some groups of patients. METHODS:Late complications and survival of 180 tunneled catheters inserted from June 2010 to December 2013 in 171 u...

journal_title：Acta biochimica Polonica

authors： Weber E,Liberek T,Wołyniec W,Gruszecki M,Rutkowski B

更新日期：2016-01-01 00:00:00

abstract：:The present study was undertaken to evaluate the protective effects of Linum usitatissimum oil (LuO) against sub-chronic Roundup (RDP)-induced toxicity and oxidative stress in rats. Rats were divided into four groups: control group (no treatment), RDP group (Roundup at 269.9 mg/kg b.w.), LuO group (0.5 g/kg b.w. of Lu...

journal_title：Acta biochimica Polonica

authors： Djaber N,Ounaceur LS,Moubine BN,Khaldi T,Rouag M,Berrouague S,Amara H,Taibi F,Boumendjel M,Boumendjel A,Messarah M

更新日期：2020-03-18 00:00:00

abstract：:Fungal infections are a growing problem in contemporary medicine, yet only a few antifungal agents are used in clinical practice. In our laboratory we proposed the enzyme L-glutamine: D-fructose-6-phosphate amidotransferase (EC 2.6.1.16) as a new target for antifungals. The structure of this enzyme consists of two dom...

journal_title：Acta biochimica Polonica

authors： Wojciechowski M,Milewski S,Mazerski J,Borowski E

更新日期：2005-01-01 00:00:00