Abstract：
:Cultivated carrot and its wild ancestor co-occur in most temperate regions of the world and can easily hybridize. The genetic basis of the process of domestication in carrot is not well understood. Recent results of an investigation on genetic diversity structure of cultivated and wild carrot and signatures for domestication using Diversity Arrays Technology (DArT) allowed identification of polymorphisms differentiating wild and cultivated accessions. We selected one of these polymorphisms, showing the strongest evidence for directional selection in the course of domestication, and converted it into a co-dominant cleaved amplified polymorphic site (CAPS) marker named cult. To achieve that, we designed site-specific primers anchored in sequences flanking the original DArT clone, amplified and sequenced the PCR products derived from cultivated and wild carrot. A PstI restriction site present in the 'cultivated' variant and absent in the 'wild' was subsequently used for routine differentiation the two variants. We validated the cult marker on 88 accessions of cultivated and wild carrot, each represented by five individuals. The allelic variant associated with the wild phenotype was only rarely observed in cultivated carrot, mostly in purple-rooted accessions originating Turkey and Iran, possibly indicating that the physical association between the diagnostic polymorphism and the putative 'domestication gene' has been broken in a group of Eastern carrots.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Macko-Podgórni A,Iorizzo M,Smółka K,Simon PW,Grzebelus Dsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
19-22issue
1eissn
0001-527Xissn
1734-154Xpii
2013_602journal_volume
61pub_type
杂志文章abstract：:The pools of Arg, Gly, His, Ile, Ser, Glu, Leu, Val and Asp were lower during photosynthesis at 1% O2 concentration. At the same time specific radioactivity of a number of amino acids--following 14CO2 incorporation--was different than that at atmospheric O2 concentration. This is probably due to the inhibition of the ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:Aberrant expression of sialic acids or altered linkage types is closely associated with malignant phenotype and metastatic potential, and can have prognostic significance in human cancer. The present study was undertaken to evaluate whether expression of sialylated derivatives on melanoma cell surface is associated wi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2015_1221
更新日期：2016-01-01 00:00:00
abstract：:The purpose of this study was to examine the effects of oxidative stress caused by hydroperoxide (H(2)O(2)) in the presence of iron ions (Fe(2+)) on mitochondria of the amoeba Acanthamoeba castellanii. We used isolated mitochondria of A. castellanii and exposed them to four levels of H(2)O(2) concentration: 0.5, 5, 15...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Glutathione-S-transferase (GST) a Phase-II drug detoxification enzyme, was detected in Setaria cervi, a bovine filarial parasite. In vitro effect of diethylcarbamazine, butylated hydroxyanisole and phenobarbitone on the GST of adult female S. cervi was assayed by the addition of these compounds in the maintenance medi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Regulation of proteolytic enzyme activity is an essential requirement for cells and tissues because proteolysis at a wrong time and location may be lethal. Proteases are synthesized as inactive or less active precursor molecules in order to prevent such inappropriate proteolysis. They are activated by limited intra- o...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：035003691
更新日期：2003-01-01 00:00:00
abstract：:Triterpene saponosides are widely distributed plant secondary metabolites characterized by relatively low systemic cytotoxicity and a range of biological activities. These include anti-inflammatory, antimicrobial, vasoprotective and antitumor properties. In particular, the ability of saponins to enhance the cytotoxici...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Public and private EST (Expressed Sequence Tag) programs provide access to a large number of ESTs from a number of plant species, including Arabidopsis, corn, soybean, rice, wheat. In addition to the homology of each EST to genes in GenBank, information about homology to all other ESTs in the data base can be obtained...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
abstract：:This review describes the recently developed GeneChip technology that provides efficient access to genetic information using miniaturised, high-density arrays of DNA or oligonucleotide probes. Such microarrays are powerful tools to study the molecular basis of interactions on a scale that would be impossible using con...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:AMP deaminase (EC 3.5.4.6) from anterior lobe of bovine pituitary has been purified for the first time. Six molecular forms of the enzyme were eluted from phosphocellulose P11 with a KC1 concentration gradient. By two stage gel chromatography individual molecular forms were purified to electrophoretic homogeneity. Com...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:A trehalose synthase gene from Deinococcus radiodurans (DSMZ 20539) containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The reco...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is the most common cause of chronic and recurrent lung infections in patients with cystic fibrosis (CF) whose sputa contain copious quantities of P. aeruginosa toxin, pyocyanin. Pyocyanin triggers tissue damage mainly by its redox cycling and induction of reactive oxygen species (ROS). The react...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). The Scatchard plots indicated the presence of two pools of binding sites both in fish and duck. These pools show inter-species differences in binding ability and the number o...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Influenza virus infection is a major source of morbidity and mortality worldwide. Due to the variable effectiveness of existing vaccines, especially in the early stages of an epidemic, antiviral drugs represent the first line of defense against the virus. Currently, there are two major classes of anti-influenza drugs ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：:Among possible causes of chronic hepatitis in adolescents most common are infections, autoimmune disorders and metabolic diseases. Thus, diagnostic procedures should be multidirectional. This study reports diagnosis and treatment difficulties in an 18-year-old male patient with hereditary hemochromatosis (HH), ulcerat...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:The use of fluorescent markers has proven to be an attractive tool in biological imaging. However, its usefulness may be confined by the cytotoxicity of the fluorescent proteins. In this article, for the first time, we have examined an influence of the antibiotics present in culture medium on cytotoxicity of the EGFP ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Polymorphisms in mitochondrial DNA (mtDNA) were analyzed in 152 samples from the Polish population using restriction enzymes AvaI, BamHI, HaeII, HpaI and PstI. Additionally, each sample was classified into the appropriate haplogroup. When required, appropriate fragments were sequenced to establish the exact polymorphi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：045104883
更新日期：2004-01-01 00:00:00
abstract：:A cytosolic acetyl-CoA hydrolase (CACH) cDNA has been isolated from mouse liver cDNA library and sequenced. Recombinant expression of the cDNA in insect cells resulted in overproduction of active acetyl-CoA hydrolyzing enzyme protein. The mouse CACH cDNA encoded a 556-amino-acid sequence that was 93.5% identical to ra...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Cytochrome c is an important electron transfer protein in the respiratory chain, shuttling electrons from cytochrome c reductase to cytochrome c oxidase. Extensive chemical modification studies indicate significant electrostatic interactions between these proteins and show that all structural and conformational change...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：045101199
更新日期：2004-01-01 00:00:00
abstract：:Previous reports indicated that long noncoding RNA 662 (LINC00662) plays a crucial role in several human cancers. Here, we studied the expression pattern of LINC00662 and explored its function in human breast cancer. The expression level of LINC00662 was determined in human breast cancer cell lines and tissues by real...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2020_5203
更新日期：2020-06-19 00:00:00
abstract：:In vitro addition of 16,16'-dimethyl prostaglandin E2 to Golgi-rich membrane fraction in final concentration of 0.1 microgram/1 mg of protein increased generally the activity of galactosyltransferase in comparison with control. The percentage of phospholipids in the whole fraction was similar in both investigated grou...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditi...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Filamentous fungi belonging to the Fusarium genus are responsible for large economic losses due to their high pathogenicity and toxigenicity. Fusarium sp. may produce variety of mycotoxins, one of them is zearalenone (ZEA). The presence of the PKS4 gene shows the possibility of zearalenone biosynthesis by Fusarium sp....
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2015_1022
更新日期：2016-01-01 00:00:00
abstract：:The arginase structural gene (agaA) from Aspergillus nidulans has been cloned and characterised. Depending on the growth conditions of the mycelium, transcripts of this gene have different 5'ends. These differences could result either from the presence of multiple transcription initiation sites or from differential pr...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Selectivity of amidination using ornithine as model amino acid was investigated in detail. The results obtained were taken advantage of for studying the reactions with other polyfunctional amino acids: beta-tyrosine, isoserine, 2,3-diaminopropionic acid, 2,6-diamino-7-hydroxyazelaic acid and with the pentapeptide amid...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:1. Susceptibility to inhibitors of neutral protease from calf thymus chromatin has been compared with that of trypsin. The chromatin protease reacts stoichiometrically with the inhibitors specific for trypsin (diisopropylfluorophosphate, tosyl-lysyl chloromethane, soybean trypsin inhibitor and Kunitz basic inhibitor f...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:The aim of this study was to evaluate the therapeutic potential of oxidative stress (OS) reduction by using pyridoindole (PI) antioxidants in adjuvant arthritis (AA). The substances tested were stobadine dipalmitate (STB) and SMe1. AA was used as animal model. The experiments included healthy animals, control arthriti...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：:The nature of genotoxic and cytotoxic effects induced by hydralazine was analyzed taking into account possible protection of cells by catalase, superoxide dismutase and dimethyl sulfoxide. For the experiments designed to evaluate the influence of scavengers on the genotoxicity expressed as the SOS induction factor the...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:To establish the identity of testis-specific hsp70 gene-related transcript, the expression pattern of the hsp70 gene family in testis and liver of rats subjected to whole body hyperthermia was investigated. In control liver two hsp70 gene-related transcripts of about 2.2 and 2.5 kb were detected. Increased body temper...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Recombination of subunits of rat liver arginase A1 and rat kidney arginase A4 yielded a product which in polyacrylamide gel electrophoresis and DEAE-cellulose chromatography separated into five proteins with arginase activity. Proteins I and V corresponded in polyacrylamide gel-electrophoresis, DEAE-cellulose chromato...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. Th...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2015_1143
更新日期：2015-01-01 00:00:00
