Abstract：
:Filamentous fungi belonging to the Fusarium genus are responsible for large economic losses due to their high pathogenicity and toxigenicity. Fusarium sp. may produce variety of mycotoxins, one of them is zearalenone (ZEA). The presence of the PKS4 gene shows the possibility of zearalenone biosynthesis by Fusarium sp. In this study, in four Fusarium graminearum and one Fusarium poae strains the presence of PKS4 genes and ZEA concentrations were determined. The presence of the PKS4 gene was confirmed by classical polymerase chain reaction (PCR) in three of four strains of F. graminearum. One strain with no PKS4 gene detected was found while still producing ZEA. In the present study, a real-time PCR assay has been successfully performed for the relative expression of Fusarium strains based on new designed primers targeting the PKS4 gene involved in ZEA biosynthesis. Result shows that P56/4 strain of F. graminearum has the highest mRNA level, in the range of 12, what correlates to the high production of this mycotoxin. In this study, a real-time PCR assay has been successfully developed for the prediction of the production of ZEA by F. graminearum strains by PCR real-time techniques based on primers targeting the gene, PKS4, involved in ZEA biosynthesis. The special significance was pointed to occurring genes polymorphism.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Misiewicz A,Goncerzewicz A,Jędrzejczak R,Zdziennicki Fdoi
10.18388/abp.2015_1022subject
Has Abstractpub_date
2016-01-01 00:00:00pages
97-102issue
1eissn
0001-527Xissn
1734-154Xpii
1682journal_volume
63pub_type
杂志文章abstract：:Temporin A (TA) and a cecropin A-temporin A hybrid peptide (CATA) were synthesized and assayed for their hemolytic, anticoagulant, and antifungal properties. CATA retains significant antifungal activity, is less hemolytic than TA, and inhibits blood coagulation. These results recommend further studies of the biologica...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The VEGF family members are important factors in promoting angiogenesis and lymphangiogenesis in malignant processes. The aim of this study was to investigate plasma concentrations of VEGF-A, VEGF-B and their soluble VEGFR-1 receptor and their diagnostic utility and potency as compared to CA 15-3 in breast cancer pati...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2018_2713
更新日期：2018-12-13 00:00:00
abstract：:Plasma membranes of rat platelets produced at normal platelet counts and during early recovery from immune-mediated thrombocytopenia were examined for the contents of carbohydrates, lipids and glycosphingolipids. Glucosylceramide, two monosialo-gangliosides and one disialo-ganglioside were found to be the major glycos...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:We report the synthesis, photochemical and photophysical properties and preliminary studies on biological effect of a new tritolylporphyrin dimer (T-D). Absorption and emission properties of T-D suggest its possible use in photodynamic therapy. T-D is capable of singlet oxygen production with 0.8 quantum yield. It als...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:1. A lytic enzyme was isolated from Vi phage III-induced lysate of Salmonella typhi, and purified about 200-fold by chromatography on IRC-50, CM-cellulose, and Sephadex G-75 columns. 2. Both E. coli B murein and muropeptide C6 were digested on incubation with the lytic enzyme. The main product of murein and muropeptid...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:This is a short survey of the adaptive mutation processes that arise in non- or slowly-dividing bacterial cells and includes: (i) bacterial models in which adaptive mutations are studied; (ii) the mutagenic lesions from which these mutations derive; (iii) the influence of DNA repair processes on the spectrum of adapti...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fu...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Annexins belong to a family of multi-functional membrane- and Ca(2+)-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca(2+)-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
abstract：:Bovine vitreous humour contains 0.6 +/- 0.04 mg of proteins per cm3. Three main protein fractions were obtained by CM-cellulose chromatography and their homogeneity was confirmed by isoelectric focusing and SDS-gel electrophoresis. The Mr and pl values of the separated fractions were: fraction I, 67,000 and 4.02; frac...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Standard ocular tumor treatment includes brachytherapy, as well as proton therapy, particularly for large melanoma tumors. However, the effects of different radiation types on the metastatic spread is not clear. We aimed at comparing ruthenium ((106)Ru, emitting β electrons) and iodine ((125)I, γ-radiation) brachyther...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:1. The amino acid composition of wheat germ EF2 differs to some extent from that of elongation factors from mammals and bacteria. 2. The purified wheat germ EF2, similarly as the factors from other sources, is active in the: EF1-dependent polymerization of phenylalanine; ribosome-dependent GTP hydrolysis; binding of g...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Nowadays, scientists may learn a lot about the organisms studied just by analyzing their genetic material. This requires the development of methods of reading genomes with high accuracy. It has become clear that the knowledge of the changes occurring within a viral genome is indispensable for effective fighting of the...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：045104983
更新日期：2004-01-01 00:00:00
abstract：:Currently, there is a search for substances that would be very well tolerated by an organism and which could contribute to the activation of the growth of Bacteroidetes and Actinobacteria strains, with simultaneous inhibition of the growth of Firmicutes. High expectations in this regard are raised with the use of fibe...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2015_1068
更新日期：2016-01-01 00:00:00
abstract：:Many nuclear genes encoding mitochondrial proteins require specific localization of their mRNAs to the vicinity of mitochondria for proper expression. Studies in Saccharomyces cerevisiae have shown that the cis-acting signal responsible for subcellular localization of mRNAs is localized in the 3' UTR of the transcript...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transp...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2017_2299
更新日期：2017-01-01 00:00:00
abstract：:The increase in the lipid peroxide level in the serum and liver of female mice after bilateral ovariectomy evidenced antioxidant activities of female hormones. This increase was abolished upon administration of female hormones. Similar increase in the level of lipid peroxide was observed in the serum of women who had ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:Geranylgeranyl diphosphate (GGPP) synthase was purified to homogeneity from bovine brain in a one-step affinity column procedure. For the construction of the affinity column, a farnesyl diphosphate (FPP) analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the ma...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Secondary structures of the N-terminal fragments of 18 preproteins were analysed taking advantage of the method of Chou & Fasman (Biochemistry, 1974, 13, 222-245; J. Mol. Biol., 1977, 115, 135-175) and the known sequences of amino acid residues. Positions of alpha-helices, beta-sheet structures and beta-turns in the l...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:The immunodiffusion cross-reactivity and competitive inhibition ELISA assays were used for immunological differentiation of latent form, cleaved form and guanidinium hydrochloride (GuHCl) induced polymer of human alpha 1-proteinase inhibitor (alpha 1-PI). Under the conditions studied, the differences between latent fo...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:A number of antiviral lectins, small proteins that bind carbohydrates found on viral envelopes, are currently in pre-clinical trials as potential drugs for prevention of transmission of human immunodeficiency virus (HIV) and other enveloped viruses, such as the Ebola virus and the coronavirus responsible for severe ac...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
abstract：:The SOS-Chromotest was used to detect DNA damage induced by two flavones: apigenin and luteolin and/or their metabolites. It was found that the compounds tested weakly induced the SOS repair system in the strain studied. ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Inhibition of jack bean activity by 2,5-dichloro-1,4-benzoquinone (DCBQ) was studied in phosphate buffer, pH 7.0. It was found that DCBQ acted as a strong, time and concentration dependent inactivator of urease. Under the experimental conditions obeyed the terms of pseudo-first-order reaction, urease was totally inact...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:Ovine β‑lactoglobulin was characterized by spectroscopic (CD), calorimetric (ITC) and X-ray structural studies. The structure of ovine β‑lactoglobulin complex with decanol showed that tight packing of molecules in the crystalline phase enforces a distortion of protein flexible loops resulting in the formation of an as...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2019_2883
更新日期：2019-12-27 00:00:00
abstract：:The myosin heavy chain (MHC) was studied by biochemical methods in the slow-twitch (soleus) and two fast-twitch leg muscles of the triiodothyronine treated (hyperthyroid), thyroidectomized (hypothyroid) and euthyroid (control) rats. The changes in the contents of individual MHC isoforms(MHC-1, MHC-2A, MHC-2B and MHC-2...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:AMP deaminase (EC 3.5.4.6) from anterior lobe of bovine pituitary has been purified for the first time. Six molecular forms of the enzyme were eluted from phosphocellulose P11 with a KC1 concentration gradient. By two stage gel chromatography individual molecular forms were purified to electrophoretic homogeneity. Com...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:We examined the molecular basis of factor IX deficiency in 53 unrelated Polish patients with hemophilia B. Heteroduplex analysis and direct sequencing of polymerase chain reaction (PCR) products were applied to identify the gene defect. Forty-three different point mutations were detected in the factor IX gene of 47 pa...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:One of the most unusual features of RNA viruses is their enormous genetic variability. Among the different processes contributing to the continuous generation of new viral variants RNA recombination is of special importance. This process has been observed for human, animal, plant and bacterial viruses. The collected d...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:A trehalose synthase gene from Deinococcus radiodurans (DSMZ 20539) containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The reco...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Monoclonal antibodies (MAb 1-7-1) directed against the isoenzymes of rat liver cytochrome P-450 induced by methylcholanthrene, inhibited benzo(a)pyrene hydroxylase less strongly at low (0.04 mM) than at high (2 mM) NADH concentration. Inhibition of NADPH dependent hydroxylation was the same irrespective of NADPH conce...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
