Abstract:
:1. The amino acid composition of wheat germ EF2 differs to some extent from that of elongation factors from mammals and bacteria. 2. The purified wheat germ EF2, similarly as the factors from other sources, is active in the: EF1-dependent polymerization of phenylalanine; ribosome-dependent GTP hydrolysis; binding of guanosine nucleotides; and ADP-ribosylation in the presence of diphtheria toxin. Fusidic acid at a concentration of 1 mM inhibits all these EF2-dependent reactions. 3. Diphtheria toxin in the presence of NAD+ inhibits polymerization of phenylalanine but does not effect GTP binding to EF2. 4. Binding of GDP to wheat germ EF2 is inhibited by ribosomes. During interaction with ribosomes, GTP in EF2-GTP complex is rapidly hydrolysed to GDP. Both GTP and 5'-guanylmethylenediphosphonate competitively inhibit formation of the ribosome-EF2-GDP complex due to the replacement of GDP from the complex. The latter is stabilized by fusidic acid.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Twardowski T,Legocki Asubject
Has Abstractpub_date
1977-01-01 00:00:00pages
21-33issue
1eissn
0001-527Xissn
1734-154Xjournal_volume
24pub_type
杂志文章abstract::Two different molecular forms of bacteriolytic endo-N-acetylmuramidase (EC 3.2.1.17) were found in the Acanthamoeba castellanii culture. The enzymes were separated by column chromatography on DEAE-Sephadex or by polyacrylamide-gel electrophoresis. In young cultures only one molecular form of the enzyme was selectively...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Regulation of proteolytic enzyme activity is an essential requirement for cells and tissues because proteolysis at a wrong time and location may be lethal. Proteases are synthesized as inactive or less active precursor molecules in order to prevent such inappropriate proteolysis. They are activated by limited intra- o...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:035003691
更新日期:2003-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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doi:
更新日期:1999-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:045101107
更新日期:2004-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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更新日期:2001-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:035001431
更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
doi:10.18388/abp.2016_1282
更新日期:2016-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1019
更新日期:2015-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:2013-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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doi:
更新日期:2007-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_1571
更新日期:2017-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
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doi:
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journal_title:Acta biochimica Polonica
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doi:
更新日期:2013-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-11-06 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00