Abstract:
:Fluorimetric method based on oxidation of the fluorogenic 6-methoxy-2-naphthaldehyde was applied to evaluate temporal and population variability of the specific activity of salivary aldehyde dehydrogenase (ALDH) and the degree of its inactivation in healthy human population. Analyzed was also its dependence on drinking and smoking habits, coffee consumption, and its sensitivity to N-acetylcysteine. Both the specific activity of salivary ALDH and the degree of its inactivation were highly variable during the day, with the highest activities recorded in the morning hours. The activities were also highly variable both intra- and interpersonally, and negatively correlated with age, and this correlation was stronger for the subgroup of volunteers declaring abstinence from alcohol and tobacco. Moderately positive correlations of salivary ALDH specific activity with alcohol consumption and tobacco smoking were also recorded (r(s) ~0.27; p=0.004 and r(s) =0.30; p=0.001, respectively). Moderate coffee consumption correlated positively with the inactivation of salivary ALDH, particularly in the subgroup of non-drinking and non-smoking volunteers. It was found that mechanical stimulation of the saliva flow increases the specific activity of salivary ALDH. The specific activity of the salivary ALDH was strongly and positively correlated with that of superoxide dismutase, and somewhat less with salivary peroxidase. The antioxidant-containing drug N-acetylcysteine increased activity of salivary ALDH presumably by preventing its inactivation in the oral cavity. Some food-related aldehydes, mainly cinnamic aldehyde and anisaldehyde, were excellent substrates of the salivary ALDH3A1 enzyme, while alkenals, particularly those with short chain, were characterized by lower affinity towards this enzyme but high catalytic constants. The protective role of salivary ALDH against aldehydes in food and those found in the cigarette smoke is discussed, as well as its participation in diminishing the effects of alcohol- and smoking-related oxidative stress.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Giebułtowicz J,Dziadek M,Wroczyński P,Woźnicka K,Wojno B,Pietrzak M,Wierzchowski Jsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
361-8issue
3eissn
0001-527Xissn
1734-154Xpii
ABPv57i3p361journal_volume
57pub_type
杂志文章abstract::Tautomerism of the cytosine molecule is discussed in connection with recent experimental matrix-isolation infrared spectroscopic measurements and recent ab initio calculations of relative stabilities of tautomers and of IR spectra for different tautomeric forms of the compound. Experimental IR spectra in the N-H and O...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
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abstract::The systemic inflammatory reaction (acute phase response) is induced by many noxious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1beta) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:10.18388/abp.2015_1106
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
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journal_title:Acta biochimica Polonica
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doi:
更新日期:1999-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:0350041129
更新日期:2003-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:2012-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
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doi:
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doi:10.18388/abp.2019_2775
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pub_type: 杂志文章
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doi:
更新日期:2007-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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更新日期:1997-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:1998-01-01 00:00:00
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doi:
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