Abstract：
:Two non-conventional analogues of ATP, 3'-deoxyadenosine-2'-triphosphate (3'-d-2'-ATP) and 2'-deoxyadenosine-3'-triphosphate (2'-d-3'-ATP), the syntheses of which are described, were examined as potential phosphate donors for the nucleoside kinases: 2'-deoxycytidine kinase (dCK), cytosolic thymidine kinase (TK1) and mitochondrial thymidine kinase (TK2). The reactions were monitored by means of a mixture of [gamma-32P]ATP and cold analogue, and/or with the use of 3H-labelled acceptors and cold donor. With dCK, using equimolar mixtures of ATP with each analogue, and dC as acceptor, phosphate transfer from 3'-d-2'-ATP and 2'-d-3'-ATP amounted to 34% and 14%, respectively. With each analogue used alone (each at concentration of 100 microM), phosphate transfer from 3'-d-2'-ATP was 55% that from ATP, and from 2'-d-3'-ATP 16%. With human TK2, and equimolar mixtures of [gamma-32P]ATP with each of the analogues, and 1 microM dT as acceptor, there was no detectable transfer from either analogue. But, when each analogue was used alone, phosphate transfer attained 11% and 5%, respectively, that for ATP alone. With the low affinity form of human TK1, and dT as acceptor, only low phosphate transfer occurred with either analogue used alone. Both compounds exhibited Michaelis-Menten kinetics (with significantly lower Vmax than ATP), while ATP exhibited cooperative kinetics with all three kinases.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Krawiec K,Kierdaszuk B,Kalinichenko EN,Mikhailopulo IA,Shugar Dsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
87-94issue
1eissn
0001-527Xissn
1734-154Xjournal_volume
45pub_type
杂志文章abstract：:Inhibition of jack bean activity by 2,5-dichloro-1,4-benzoquinone (DCBQ) was studied in phosphate buffer, pH 7.0. It was found that DCBQ acted as a strong, time and concentration dependent inactivator of urease. Under the experimental conditions obeyed the terms of pseudo-first-order reaction, urease was totally inact...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
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更新日期：2003-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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更新日期：2009-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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更新日期：2002-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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doi：045104883
更新日期：2004-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2017_2331
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pub_type： 杂志文章
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：035003573
更新日期：2003-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2015_1013
更新日期：2016-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2005-11-06 00:00:00
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journal_title：Acta biochimica Polonica
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doi：
更新日期：2008-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2016_1265
更新日期：2017-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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doi：
更新日期：1987-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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更新日期：2006-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
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更新日期：2012-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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doi：
更新日期：2013-01-01 00:00:00
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journal_title：Acta biochimica Polonica
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doi：
更新日期：2012-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
