Abstract:
:The pathogenicity of RHDV (rabbit haemorrhagic disease virus) is mainly associated with its affinity to blood vessels, with causing disseminated intravascular coagulations (DIC), and with the stimulation of the host immune system. Moreover, there are implications suggesting that apoptosis may be a pivotal process in understanding the basis of viral haemorrhagic disease in rabbits - a serious infectious disease causing mortality to wild and domestic rabbits. The aim of this study is to evaluate, by means of flow cytometry, the dynamics of apoptosis in peripheral blood granulocytes and lymphocytes in rabbits experimentally infected with seven different strains of RHDV and so-called antigenic variants of RHDV denominated as RHDVa, i.e.: Hungarian 24V/89, 1447V/96, 72V/2003; Austrian 01-04, 237/04, V-412 and French 05-01. The results showed that all of the RHDV and RHDVa strains cause an increase in the number of apoptotic cells throughout the infection, which might indicate the need for further analysis of the importance of this process.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Niedźwiedzka-Rystwej P,Hukowska-Szematowicz B,Tokarz-Deptuła B,Trzeciak-Ryczek A,Działo J,Deptuła Wsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
65-9issue
1eissn
0001-527Xissn
1734-154Xpii
2012_347journal_volume
60pub_type
杂志文章abstract::Rat duodenum and jejunum were found to contain three forms of alkaline phosphatase, F1, F2 and F3, and ileum two forms of this enzyme, F1 and F2. The procedure for separation of phosphatase F1, F2 and F3 from jejunum is presented. ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::The present paper investigates the enhancement of the therapeutic effect of Paclitaxel (a potent anticancer drug) by increasing its cellular uptake in the cancerous cells with subsequent reduction in its cytotoxic effects. To fulfill these goals the Paclitaxel (PTX)-Biotinylated PAMAM dendrimer complexes were prepared...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_2331
更新日期:2018-01-01 00:00:00
abstract::Two structurally related compounds, phenyl dithiocarbamate sodium salt (I) and p-phenylene-bis (dithiocarbamate) sodium salt (II) were prepared by reaction of the parent aniline and p-phenylenediamine with CS₂ in the presence of sodium hydroxide. These water soluble compounds were characterized by spectroscopic techni...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The amniotic fluid (AF) was fractionated by dialysis, gel filtration and SDS/PAGE, and submitted to the assay of collagenous constituents. The collagenous character of peptides and proteins of amniotic fluid was confirmed by hydroxyproline (Hyp) assay and treatment with bacterial collagenase followed by electrophoresi...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::In situ PCR and in situ reverse transcription PCR (RT-PCR) were applied to discriminate between latent and productive infection of human cytomegalovirus (HCMV) in leukocytes. We investigated 28 samples, in which viral pp65 antigen was detected only in the cytoplasm of leukocytes. Additionally we assayed 12 specimens l...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Angioarrestin is a recently described anti-angiogenic protein whose expression is down-regulated in solid tumours of various origins. It has a sequence identical to angiopoietin related protein-1. In this study we investigated anti-tumour properties of angioarrestin in B16 (F10) melanoma tumour model. We constructed a...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::This review describes the recently developed GeneChip technology that provides efficient access to genetic information using miniaturised, high-density arrays of DNA or oligonucleotide probes. Such microarrays are powerful tools to study the molecular basis of interactions on a scale that would be impossible using con...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2001-01-01 00:00:00
abstract::Murine leukemia L1210 cells contain active glucosamine 6-phosphate synthase inhibited by N3-4-methoxyfumaroyl-L-2,3-diaminopropanoic acid (FMDP). However, FMDP-peptides do not exhibit any cytotoxicity against these cells, HeLa S3 human cervical carcinoma cells, and LL2 murine Lewis lung carcinoma cells. It is suggeste...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The aim of this study was to analyse the correlation between HPV16 E6 variants and the physical status of viral genome (integrated, mixed, episomal) among patients with cervical cancer (n=40) and low-grade squamous intraepithelial lesions - LSIL (n=40). The study was performed on 80 HPV16 positive samples. HPV16 E6 va...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1364
更新日期:2017-01-01 00:00:00
abstract::1. Gluconeogenesis from glutamine, fumarate, pyruvate, glutamine plus fumarate, and glutamine plus pyruvate, was generally higher at pH 7.1 than at pH 7.4 and 7.7, whereas ammoniagenesis did not depend on the pH of the medium. 2. The intermediates of the Krebs cycle decreased ammonia formation from glutamine, raising ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_1571
更新日期:2017-01-01 00:00:00
abstract::The relationships between bone turnover, the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis and vitamin D are complex, but still not fully explained. The GH/IGF-1 axis and vitamin D can mutually modulate each other's metabolism and influence the activation of cell proliferation, maturation, and mineraliza...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_2541
更新日期:2018-01-01 00:00:00
abstract::Triterpene saponins (saponosides) are found in a variety of higher plants and display a wide range of pharmacological activities, including expectorant, anti-inflamatory, vasoprotective, gastroprotective and antimicrobial properties. Recently, a potential anticancer activity of saponins has been suggested by their cyt...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:10.18388/abp.2015_1089
更新日期:2015-01-01 00:00:00
abstract::The paradigm of annexins as phospholipid-binding proteins interacting with membranes in a calcium-dependent manner has been recently questioned in light of observations that some annexin isoforms may behave like membrane integral proteins or remain associated with their target membranes at low, resting, concentrations...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1998-01-01 00:00:00
abstract::Bovine vitreous humour contains 0.6 +/- 0.04 mg of proteins per cm3. Three main protein fractions were obtained by CM-cellulose chromatography and their homogeneity was confirmed by isoelectric focusing and SDS-gel electrophoresis. The Mr and pl values of the separated fractions were: fraction I, 67,000 and 4.02; frac...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Eight polymorphic restriction enzyme sites at phenylalanine hydroxylase locus from the parental chromosomes in Polish families with phenylketonuria were analyzed. Among 28 chromosomes studied, we identified haplotypes found within the Danish population. Haplotype 2 has been found in 25% of affected alleles. One of the...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The MAP family includes large proteins like MAP-1A, MAP-1B, MAP-1C, MAP-2, and MAP-4 and smaller components like tau and MAP-2C. This article focuses on the relevant aspects of RAP55/LSM14 position with emphasis on its role in mitotic spindle formation and stability. In this context, the localization of RNA associated...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1107
更新日期:2015-01-01 00:00:00
abstract::New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fulfilled in ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::The aim of the study presented here was an attempt to answer the question posed in the title: Is the mechanism of nitroglycerin tolerance associated with aldehyde dehydrogenase (ALDH) activity? Here, we investigated the effect of administration (separately or jointly) of lipoic acid (LA), nitroglycerin (GTN), and disu...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2019_2908
更新日期:2019-12-28 00:00:00
abstract::Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology. We have...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1261
更新日期:2016-01-01 00:00:00
abstract::Gene promoters contain several classes of functional sequence elements (cis elements) recognized by protein agents, e.g. transcription factors and essential components of the transcription machinery. Here we describe a common DNA regulatory element (tandem TCTCGCGAGA motif) of human TATA-less promoters. A combination ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Hepatitis C virus (HCV) infection is a global health problem which can lead to liver cirrhosis or hepatocellular carcinoma in one-fifth of chronically infected patients. MATERIALS AND METHODS:The study group consisted of 123 patients: 90 with HCV mono- and 33 with HIV/HCV co-infection, who were treated wi...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::Potassium permanganate oxidation of pyrimidine bases is often used to probe single-stranded regions in functional DNA-protein complexes. However, so far reactivity of these bases in double-stranded DNA has not been studied quantitatively. We have investigated the kinetics of oxidation of pyrimidines in supercoiled pDS...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-in...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_1622
更新日期:2018-01-01 00:00:00
abstract::Kainate is a glutamate analog that produces neuronal excitation resulting in seizures within hours following its intraperitoneal injection into adult rats. Then, at 2-3 days after the treatment, neurodegeneration of apoptotic character can be observed in limbic system. As a consequence, plastic reorganization and glia...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
abstract::Liposomes made of sphingomyelin were found to inhibit both ATP-activated and non-activated AMP deaminase from pig brain, in contrast to liposomes made of egg yolk phosphatidylcholine which exhibited an activating effect on the ATP-activated enzyme, being without effect on AMP deaminase in the absence of ATP. Dioleoylp...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Microbiological purity control of food products is of great importance in the food industry. Contaminated food is often characterized by a deteriorated taste, smell, and appearance, and when consumed, it can pose a threat to human health and life. Also, contamination incurs huge financial losses to the food industry. ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2020_5380
更新日期:2020-09-14 00:00:00
abstract::Influenza virus infection is a major source of morbidity and mortality worldwide. Due to the variable effectiveness of existing vaccines, especially in the early stages of an epidemic, antiviral drugs represent the first line of defense against the virus. Currently, there are two major classes of anti-influenza drugs ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract::Several studies employed the genome-wide association (GWA) analysis of single-nucleotide polymorphisms (SNPs) to identify susceptibility regions in colorectal cancer (CRC). However, the functional studies exploring the role of associating SNPs with cancer biology are limited. Herein, using chromatin immunoprecipitatio...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2019_2775
更新日期:2019-09-17 00:00:00